Characterization and analysis of the antidiabetic activities of sulphated polysaccharide extract from Caulerpa lentillifera

Abstract

Caulerpa lentillifera is a type of green seaweed that is cultivated in tropical and subtropical areas. The objectives of this study were to determine the characteristics of the sulfated polysaccharides from C. lentillifera and evaluate its antidiabetic activity. In the initial process of this study, samples were macerated with ethanol (1:10). Then, the maceration residue was extracted with an accumulator at 75 °C for three hours. The crude extract yield was 4.16% based on weight seaweed. Ion chromatography purification with DEAE-Sepharose resin provided a yield of 14.8% of crude extract. The monomer analysis of C. lentillifera from the crude extract and purified extract revealed that galactose monomers were dominant and glucose was a minor component. The total carbohydrate and sulfate contents of purified C. lentillifera were higher than those of crude C. lentillifera. Bioactivity tests revealed that purified polysaccharides had higher antidiabetic activity against α-glucosidase enzyme than crude ones with IC50 values of 134.81± 2.0 µg/mL. Purified sulfated polysaccharides of C. lentillifera could potentially be used as an antidiabetic medication.

Amelioration of obesity-related metabolic disorders via supplementation of Caulerpa lentillifera in rats fed with a high-fat and high-cholesterol diet

“…C. lentillifera contains various bioactive molecules that can contribute to its anti-hyperglycemic activity, including sulfated polysaccharides and monosaccharides. A previous study by Fajriah et al showed that purified polysaccharides of C. lentillifera had significant inhibition capability against α-glucosidase ( 41 ). A -glucosidase is an enzyme located in the brush borders of the small intestine, which operates by cleaving disaccharides into glucose to be further absorbed ( 42 ).…”