Polyphenols rich fraction of Dicranopteris linearis promotes fibroblast cell migration and proliferation in vitro

Ponnusamy, Yasodha; Chear, Nelson Jeng Yeou; Ramanathan, Surash; Lai, Choon-Sheen

doi:10.1016/j.jep.2015.03.062

Cited by 29 publications

(20 citation statements)

References 22 publications

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“…To establish the pharmacological effects of PAE and validate the optimal extraction process, the HSF were used in the in vitro wound healing model for evaluation of the cellular and molecular effects of PAE. The fibroblasts enhanced proliferation and migration after treatment with PAE (0.3125 mg/mL) for 48 h. In addition, PAE significantly increased the healing rates and reduced the healing time by enhancing epithelial repair, follicular regeneration, and fibrous tissue proliferation following cutaneous thermal injury in vivo [24–26]. …”

Section: Discussionmentioning

confidence: 99%

Periplaneta americana Extracts Promote Skin Wound Healing via Nuclear Factor Kappa B Canonical Pathway and Extracellular Signal-Regulated Kinase Signaling

Qin

Xie

Zhang

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Periplaneta americana extracts (PAEs) exhibit wound healing properties. However, the underlying molecular mechanisms are not well understood. Here, we treated human skin fibroblasts (HSF) with PAE and the proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The wound healing and transwell migration assays were used to detect cell migration. Nuclear factor kappa B (NF-κB) and extracellular signal-regulated kinase (ERK) pathways were analyzed by Western blot (WB). Immunofluorescence staining was used to detect the key molecular localization in the cells. The results showed that PAE enhanced the proliferation and migration of HSF cells. The expression and activation of key proteins such as RelA and p-ERK were increased in NF-κB and ERK pathways followed by nuclear translocation. In vivo, both WB and immunohistochemical (IHC) staining showed that PAE enhanced p-IκBα and p-ERK activation and the nuclear translocation of RelA. Our study suggests that the protective function of PAE is mediated via enhanced NF-κB and ERK signaling.

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Section: Discussionmentioning

confidence: 99%

Periplaneta americana Extracts Promote Skin Wound Healing via Nuclear Factor Kappa B Canonical Pathway and Extracellular Signal-Regulated Kinase Signaling

Qin

Xie

Zhang

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…Hydrogen peroxide was used for induction of oxidative stress as described by Balekar et al [18] and Ponnusamy et al [19]. The L929 fibroblast cells were seeded at a density of 5 × 10 3 cells/well into a 96-well plate in DMEM supplemented with 10% FBS and incubated at 37°C, in a humidified 5% CO 2 atmosphere overnight.…”

Section: Methodsmentioning

confidence: 99%

In VitroEvaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract

Pitz

Pereira

Blasius

et al. 2016

Oxidative Medicine and Cellular Longevity

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Jaboticaba is a fruit from a native tree to Brazil, Plinia peruviana. Jaboticaba peels are an important source of antioxidant molecules such as phenolic compounds. This study aimed to evaluate in vitro the activity of a hydroalcoholic extract of jaboticaba fruit peels (HEJFP) in wound healing processes and antioxidant activity in murine fibroblasts (L929 cell line). HEJFP concentrations (0.5, 1, 5, 10, 25, 50, 100, and 200 µg/mL) were tested in MTT assay and cell proliferation was verified at 100 µg/mL after 24 h and at 25, 50, and 100 µg/mL after 48 h of extract exposure. Evaluation of antioxidant activity was performed at 0.5, 5, 25, 50, and 100 µg/mL HEJFP concentrations. Cell treatment with HEJFP at 25, 50, and 100 µg/mL for 24 h followed by H2O2 exposure for 3 h showed a strong cytoprotective effect. In vitro scratch wound healing assay indicated that none of tested HEJFP concentrations (0.5, 5, 25, 50, and 100 µg/mL) were capable of increasing migration rate after 12 h of incubation. These results demonstrate a positive effect of HEJFP on the wound healing process on L929 fibroblasts cell line, probably due to the antioxidant activity exhibited by phytochemicals in the extract.

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“…These facts might explain why flavonoid was not detected during the qualitative phytochemical screening stage. It is believed that Peak 1 might possibly belong to the phenolic glycosides group particularly of the flavonol 3-O-glycosides group as reported by Raja et al [65], Chen et al [66], and Ponnusamy et al [67]. Further phytoconstituents analyses need to be carried out and the possible bioactive compounds in AQDL need to identified.…”

Section: Discussionmentioning

confidence: 93%

Aqueous Partition of Methanolic Extract of Dicranopteris linearis Leaves Protects against Liver Damage Induced by Paracetamol

et al. 2019

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This study aimed to determine the antioxidant and hepatoprotective activities of semi-purified aqueous partition obtained from the methanol extract of Dicranopteris linearis (AQDL) leaves against paracetamol (PCM)-induced liver intoxication in rats. The test solutions, AQDL (50, 250, and 500 mg/kg), were administered orally to rats (n = 6) once daily for seven consecutive days followed by the hepatotoxicity induction using 3 g/kg PCM (p.o.). Blood was collected for serum biochemical parameters analysis while the liver was collected for histopathological examination and endogenous antioxidant enzymes analysis. AQDL was also subjected to antioxidant determination and phytochemical analysis. Results obtained show that AQDL possessed high total phenolic content (TPC) value and remarkable radical scavenging activities. AQDL also significantly (p < 0.05) reduced the liver weight/body weight (LW/BW) ratio or serum level of ALT, AST, and total bilirubin while significantly (p < 0.05) increase the level of superoxide dismutase (SOD) and catalase (CAT) without affecting the malondialdehyde (MDA) in the liver indicating its hepatoprotective effect. Phytoconstituents analyses showed only the presence of saponins and triterpenes, but lack of flavonoids. In conclusion, AQDL exerts hepatoprotective activity via its high antioxidant potential and ability to modulate the endogenous enzymatic antioxidant defense system possibly via the synergistic action of saponins and triterpenes.

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Polyphenols rich fraction of Dicranopteris linearis promotes fibroblast cell migration and proliferation in vitro

Cited by 29 publications

References 22 publications

Periplaneta americana Extracts Promote Skin Wound Healing via Nuclear Factor Kappa B Canonical Pathway and Extracellular Signal-Regulated Kinase Signaling

Periplaneta americana Extracts Promote Skin Wound Healing via Nuclear Factor Kappa B Canonical Pathway and Extracellular Signal-Regulated Kinase Signaling

In VitroEvaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract

Aqueous Partition of Methanolic Extract of Dicranopteris linearis Leaves Protects against Liver Damage Induced by Paracetamol

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