In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract

Abstract

Jaboticaba is a fruit from a native tree to Brazil, Plinia peruviana. Jaboticaba peels are an important source of antioxidant molecules such as phenolic compounds. This study aimed to evaluate in vitro the activity of a hydroalcoholic extract of jaboticaba fruit peels (HEJFP) in wound healing processes and antioxidant activity in murine fibroblasts (L929 cell line). HEJFP concentrations (0.5, 1, 5, 10, 25, 50, 100, and 200 µg/mL) were tested in MTT assay and cell proliferation was verified at 100 µg/mL after 24 h and at 25, 50, and 100 µg/mL after 48 h of extract exposure. Evaluation of antioxidant activity was performed at 0.5, 5, 25, 50, and 100 µg/mL HEJFP concentrations. Cell treatment with HEJFP at 25, 50, and 100 µg/mL for 24 h followed by H2O2 exposure for 3 h showed a strong cytoprotective effect. In vitro scratch wound healing assay indicated that none of tested HEJFP concentrations (0.5, 5, 25, 50, and 100 µg/mL) were capable of increasing migration rate after 12 h of incubation. These results demonstrate a positive effect of HEJFP on the wound healing process on L929 fibroblasts cell line, probably due to the antioxidant activity exhibited by phytochemicals in the extract.

Hydrolysed collagen from Lates calcarifer skin: its acute toxicity and impact on cell proliferation and collagen production of fibroblasts

“…Nevertheless, there was no obvious effect on cell proliferation of HC/VitC mixtures at 1:1 and 1:2. Plinia peruviana extract having antioxidative activities was reported to demonstrate the potential for stimulation of L929 cell proliferation and wound healing properties (Pitz et al ., ). Liu et al .…”

“…Nevertheless, there was no obvious effect on cell proliferation of HC/VitC mixtures at 1:1 and 1:2. Plinia peruviana extract having antioxidative activities was reported to demonstrate the potential for stimulation of L929 cell proliferation and wound healing properties (Pitz et al, 2016). Liu et al (2014) found that collagen peptides could promote osteoblast proliferation by inducing cell cycle arrest in G2/S phase (DNA synthesis) performed by flow cytometry scanning.…”

Evaluation of the Antioxidant and Wound-Healing Properties of Extracts from Different Parts of Hylocereus polyrhizus

“…The scratch assay is an imitative form that stimulates a wound in-vitro and evaluates the cell migration rate. When the cell monolayer is disrupted cell-cell interactions disappear and the secretion of growth factors and cytokines is increased at the wound edge, initiating migration and proliferation of cells [16]. Interestingly, none of the extracts were able to promote cell proliferation at concentrations of 250 µg/mL to 2000 µg/mL over a 24 h period.…”

“…This process may get impaired when cells secret reactive oxygen species (ROS) [15]. ROS can degrade extracellular matrix proteases and damage fibroblasts and keratinocytes [16].…”

In vivo wound healing potential and HPLC-PDA-ESI-MS profiling ofZizyphus lotusL. (Desf.) leaves methanol extract

“…However, excess neutrophils and ROS degrades extracellular matrix proteins and damages dermal fibroblasts and keratinocytes function by affecting cellular membranes, DNA, proteins, and lipids (Pitz et al, 2016;S€ untar et al, 2012).…”