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The world coffee consumption has been growing for its appreciated taste and its beneficial effects on health. The residual biomass of coffee, originated in the food industry after oil extraction from coffee beans, called coffee beans residual press cake, has attracted interest as a source of compounds with antioxidant activity. This study investigated the chemical composition of aqueous extracts of coffee beans residual press cake (AE), their antioxidant activity, and the effect of topical application on the skin wound healing, in animal model, of hydrogels containing the AE, chlorogenic acid (CGA), allantoin (positive control), and carbopol (negative control). The treatments' performance was compared by measuring the reduction of the wound area, with superior result (p < 0.05) for the green coffee AE (78.20%) with respect to roasted coffee AE (53.71%), allantoin (70.83%), and carbopol (23.56%). CGA hydrogels reduced significantly the wound area size on the inflammatory phase, which may be associated with the well known antioxidant and anti-inflammatory actions of that compound. The topic use of the coffee AE studied improved the skin wound healing and points to an interesting biotechnological application of the coffee bean residual press cake.

OBJETIVO: Avaliar a relação entre o uso de vestimenta de proteção radiológica e a diminuição da dose absorvida de radiação ionizante, reforçando a eficácia do seu uso tanto para pacientes quanto para indivíduos ocupacionalmente expostos. MATERIAIS E MÉTODOS: O estudo foi desenvolvido utilizando-se o método de revisão integrativa de literatura, e teve como materiais: 21 artigos, 2 livros, 1 tese, 1 trabalho de conclusão de curso, 1 programa de computador, 4 pesquisas em base de dados (Instituto Brasileiro de Geografia e Estatística e Departamento de Informática do Sistema Único de Saúde) e 2 diretrizes de proteção radiológica. RESULTADOS: A utilização da vestimenta de proteção radiológica, teoricamente, reduz 86% a 99% a dose absorvida. Na prática, a redução nos pacientes pode ser de 88% na radiologia convencional e chegar a 95% no exame tomográfico. Nos indivíduos ocupacionalmente expostos, a redução durante um cateterismo cardíaco é em torno de 90% e durante uma cirurgia ortopédica é de 75%. CONCLUSÃO: Conforme demonstrado em várias pesquisas, o uso de vestimenta de proteção radiológica é eficaz e de baixo custo e reduz a dose desnecessária nos pacientes e nos indivíduos ocupacionalmente expostos. Logo, sua utilização é necessária para a implementação de um efetivo programa de proteção radiológica em um serviço de radiodiagnóstico.

Jaboticaba is a fruit from a native tree to Brazil, Plinia peruviana. Jaboticaba peels are an important source of antioxidant molecules such as phenolic compounds. This study aimed to evaluate in vitro the activity of a hydroalcoholic extract of jaboticaba fruit peels (HEJFP) in wound healing processes and antioxidant activity in murine fibroblasts (L929 cell line). HEJFP concentrations (0.5, 1, 5, 10, 25, 50, 100, and 200 µg/mL) were tested in MTT assay and cell proliferation was verified at 100 µg/mL after 24 h and at 25, 50, and 100 µg/mL after 48 h of extract exposure. Evaluation of antioxidant activity was performed at 0.5, 5, 25, 50, and 100 µg/mL HEJFP concentrations. Cell treatment with HEJFP at 25, 50, and 100 µg/mL for 24 h followed by H2O2 exposure for 3 h showed a strong cytoprotective effect. In vitro scratch wound healing assay indicated that none of tested HEJFP concentrations (0.5, 5, 25, 50, and 100 µg/mL) were capable of increasing migration rate after 12 h of incubation. These results demonstrate a positive effect of HEJFP on the wound healing process on L929 fibroblasts cell line, probably due to the antioxidant activity exhibited by phytochemicals in the extract.

Glyphosate (N-phosphonomethyl-glycine) (GLY) is the active ingredient of the most used herbicides in the world. GLY is applied in formulated products known as glyphosate-based herbicides (GBH), which could induce effects that are not predicted by toxicity assays with pure GLY. This herbicide is classified as organophosphorus compound, which is known to induce neurotoxic effects. Although this compound is classified as non-neurotoxic by regulatory agencies, acute exposure to GBH causes neurological symptoms in humans. However, there is no consensus in relation to neurotoxic effects of GBH. Thus, the aim of this study was to investigate the neurotoxic effects of the GBH in the zebrafish Danio rerio, focusing on acute toxicity, the activity and transcript levels of mitochondrial respiratory chain complexes, mitochondrial membrane potential, reactive species (RS) formation, and behavioral repertoire. Adult zebrafish were exposed in vivo to three concentrations of GBH Scout, which contained GLY in formulation (fGLY) (0.065, 1.0 and 10.0 mg L fGLY) for 7 d, and an in vitro assay was performed using also pure GLY. Our results show that GBH induced in zebrafish brain a decrease in cell viability, inhibited mitochondrial complex enzymatic activity, modulated gene expression related to mitochondrial complexes, induced an increase in RS production, promoted hyperpolarization of mitochondrial membrane, and induced behavioral impairments. Together, our data contributes to the knowledge of the neurotoxic effects of GBH. Mitochondrial dysfunction has been recognized as a relevant cellular response that should not be disregarded. Moreover, this study pointed to the mitochondria as an important target of GBH.

A educação a distância (EaD) historicamente tem se apropriado de novas mídias etecnologias. Com a utilização de mídias sociais e games por estudantes de diversas faixas etárias essas tecnologias demonstram potencialidades para os processos de ensino e  aprendizagem,  especialmente  no  caso  da  EaD,  que  atualmente  acontece principalmente no meio online. É abordada nesse artigo a relação entre a educação a distância, a mídia e a tecnologia, refletindo sobre as oportunidades que as mídias sociais e a gamificação podem oferecer para potencializar os processos de ensino aprendizagem e engajar os alunos através do uso desses novos recursos tecnológicos. Baseando-se nas teorias de aprendizagem sócio-construtivismo e cognição situada, são apresentadas possibilidades de utilização das mídias sociais e de processos gamificados em ambientes virtuais de aprendizagem para a EaD.

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