Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia

Hartatik, Tety; Volkandari, Slamet Diah; Rachman, Mifta Pratiwi; Sumadi, .

doi:10.1016/j.proenv.2013.02.017

Cited by 9 publications

(9 citation statements)

References 7 publications

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“…The GH L allele was predominantly found in both of cattle populations This is same with previous studies in the Growth Hormone gene in cattle, Reis et al 2001;Silveira et al 2008;Mu'in et al 2007;Hartatik et al 2013;Volkandari et al 2013;Moravcikova et al 2012;Akcay et al 2015).…”

Section: Genotype and Allele Frequenciessupporting

confidence: 87%

Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia

et al. 2016

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. Blood samples were collected from 66 individuals consist of 15 Pesisir cattle, 15 SimPes cattle, 15 SimPO cattle, 15 Bali cattle and 6 PO cattle. DNA was extracted from each blood samples after SDS-proteinase K digestion, and used for PCR-amplification for a region of growth hormone gene (211 bp), and then the PCR products were analyzed for restriction fragment length polymorphism (RFLP) using AluI restriction enzyme. The results showed that GH gene of Pesisir, PO and Bali cattle are monomorphic, which frequencies of L allele was 1.00 and V allele was 0.000 while these LL genotype was 1.000. Frequency of L and V alleles in SimPO and SimPes cattle were 0.634, 0.366 and 0.700, 0.300, respectively. SimPO and SimPes cattle were polymorphic, LL and LV of SimPO cattle was 0.733 and 0.267 as well as SimPes cattle which LL and LV was 0.600 and 0.400, respectively. The correlation between genotype and the performance (body weight and body size) was not significant. The present study indicates that polymorphism of growth hormone gene in AluI site could not yet be used as a molecular marker for body weight and body size of beef cattle.

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Section: Genotype and Allele Frequenciessupporting

confidence: 87%

Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia

et al. 2016

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“…Three patterns (genotypes) were produced as the result of AluI restriction site (AGCT) such as VV (211 bp), LV (211, 159 and 52 bp) and LL (159 and 52 bp) band pattern could be distinguished on the gel (Hartatik et al, 2013;Mu'in, 2008).…”

Section: Genotype Allel Frequency and Data Analysismentioning

confidence: 99%

Associations Between Polymorphism of Growth Hormone Gene With Milk Production, Fat and Protein Content in Friesian Holstein Cattle

Hartatik

Kurniawati

Adiarto

2015

J. Indonesian Trop. Anim. Agric.

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ABSTRAKPenelitian ini bertujuan untuk menganalisis hubungan polimorfisme gen hormon pertumbuhan (leu/val) dan produksi susu pada sapi Friesian Holstein. Sampel penelitian berasal dari 62 ekor sapi yang diambil dari dua kelompok sapi Friesian Holstein (dari New Zealand=19 ekor and Australia=43 ekor). Polymerase Chain Reaction (PCR) yang dilanjutkan dengan Restriction Fragment Length Polymorphism (RFLP) menggunakan enzim restriksi AluI untuk menentukan genotip sapi. Genotip ditentukan berdasarkan target gen GH 211 bp yang terletak pada intron 4 dan exon 5 dari gen hormon pertumbuhan. Genotypes Leu/Leu (LL) pada kedua grup menunjukkan frekuensi yang lebih tinggi dibandingkan dengan genotip Leu/Val (LV). Sapi Friesian Holstein dari New Zealand memperlihatkan genotip LL dan LV sebesar 84% dan 16%. Sapi Friesian Holstein dari Australia memiliki genotip LL dan LV sebesar 79% and 21%. Hubungan antara polimorfisme Leu/Val pada gen hormon pertumbuhan terhadap produksi susu, kadar lemak dan protein tidak menunjukkan pengaruh yang nyata pada kedua grup. Berdasarkan pengelompokan asal sapi, hasil penelitian menunjukkan perbedaan yang nyata pada kadar lemak dan protein susu. Kadar lemak dan protein susu pada sapi FH yang diimpor dari Australia lebih tinggi dibandingkan dengan kadar lemak dan protein susu pada sapi FH yang diimpor dari New Zealand. Kata kunci: Friesian Holstein import, gen hormon pertumbuhan, produksi susu, kadar lemak dan protein ABSTRACTThe aim of the research was to determine the associations between polymorphism of the bovine growth hormone (GH) gene (Leu/Val) and milk production of Friesian Holstein Cattle. A total of 62 cows which consist of two Friesian Holstein cattle groups (from New Zealand=19 heads and Australia=43 heads) were used for the research. We performed the Polymerase Chain Reaction and followed by Restriction Fragment Length Polymorphism using AluI restriction enzyme. The genotype was observed base on the target gene GH 211 bp in the part of intron 4 and exon 5 of growth hormone gene. The frequencies of genotypes LL were found higher than genotype LV in both groups. Friesian Holstein cattle from New Zealand showed the genotype LL and LV as 84% and 16%, respectively. Friesian Holstein cattle from Australia show the genotype LL and LV as 79% and 21%, respectively. The association between Leu/Val polymorphism on milk production, fat and protein content in both groups did not show the significant effect. Base on two groups of the origin of cattle, the result showed the significant different on fat and protein content of milk. Fat and protein contents of milk were higher in breed of FH imported from Australia compared to those in breed of FH imported from New Zailand.

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“…In the SO cattle, the V allele (recognized with C||G at position 1642) occurred at a low frequency (0.06). The low V allele frequency of SNP g.1642C>G was also reported in Brahman cattle (Thomas et al 2007) and Indonesian Ongole-grade cattle (Hartatik et al 2013). Nevertheless, the V allele did not exist Madura cattle (Volkandari et al 2013) and Pesisir cattle (Putra et al 2016).…”

Section: Resultsmentioning

confidence: 75%

“…The mutation existed in exon 5 at position 1642 was also reported to occur both in the Bos indicus and Bos taurus cattle (Thomas et al 2007;Hartatik et al 2013). The SNP g.1642C>G causes an amino acid change from leucine (Leu) to valine (Val).…”

Section: Resultsmentioning

confidence: 99%

“…The GH gene polymorphism was intensively studied in many cattle breeds including local Indonesian cattle breeds (Paputungan et al 2012;Hartatik et al 2013;Putra et al 2014;Putra et al 2016) but limited reports for the Indonesian SO cattle GH gene. Up to present, information about the SO cattle GH gene polymorphism is limited only in the intron region (Agung et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene

et al. 2018

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Agung PP, Putra WPB, Anwar S, Wulandari AS, Zein MSA, Said S, Sudiro A. 2018. Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene. Biodiversitas 19: 676-681. The Sumba Ongole cattle (Bos indicus) is one of the local Indonesian cattle breeds and has excellent potential to gain higher carcass yield compared to other local cattle breeds in Indonesia. The Growth Hormone (GH) gene was a potential target for molecular study due to its importance as growth regulating hormone. This study was aimed to determine Single Nucleotide Polymorphism (SNP) profile of the GH gene in the Sumba Ongole (SO) cattle using PCR and DNA sequencing methods from 34 individual DNA samples. The results from this study showed that there are 28 SNPs identified within the GH gene; Twenty one SNPs were found to occur in the intron region, 4 SNPs in the exon region, and 3 SNPs in the 3’ end region. There were 17 SNPs in the SO cattle GH gene were caused by novel mutation. The novel SNP g.1395insC was found only in the SO cattle. Moreover, the frequency of the SNP g.1395insC was the highest among other novel SNPs in the SO cattle GH gene. The moderate PIC value (0.25<PIC<0.50) were found in two novel SNPs i.e. SNP g.1415C>G (intron 4), and SNP g.1526A>G (intron 4). The GH gene in the SO cattle was polymorphic and the 17 novel SNPs need to be further validated.

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Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia

Cited by 9 publications

References 7 publications

Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia

Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia

Associations Between Polymorphism of Growth Hormone Gene With Milk Production, Fat and Protein Content in Friesian Holstein Cattle

Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene

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