Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia

Abstract

. Blood samples were collected from 66 individuals consist of 15 Pesisir cattle, 15 SimPes cattle, 15 SimPO cattle, 15 Bali cattle and 6 PO cattle. DNA was extracted from each blood samples after SDS-proteinase K digestion, and used for PCR-amplification for a region of growth hormone gene (211 bp), and then the PCR products were analyzed for restriction fragment length polymorphism (RFLP) using AluI restriction enzyme. The results showed that GH gene of Pesisir, PO and Bali cattle are monomorphic, which frequencies of L allele was 1.00 and V allele was 0.000 while these LL genotype was 1.000. Frequency of L and V alleles in SimPO and SimPes cattle were 0.634, 0.366 and 0.700, 0.300, respectively. SimPO and SimPes cattle were polymorphic, LL and LV of SimPO cattle was 0.733 and 0.267 as well as SimPes cattle which LL and LV was 0.600 and 0.400, respectively. The correlation between genotype and the performance (body weight and body size) was not significant. The present study indicates that polymorphism of growth hormone gene in AluI site could not yet be used as a molecular marker for body weight and body size of beef cattle.

Genotype analysis of partial growth hormone gene (GH891│MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle

“…DNA was isolated according to the procedure conducted by Putra et al (2016). Two hundreds microliters of blood sample in 1.5 mL microtubes were added with 800 µL of buffer A ((0.32 M Sucrose (Himedia, India); 10 mM TrisHCL pH 7.5; 5 mM MgCl 2 (Merck, Germany), 1% Triton X-100 (applichem); DDW)), and it was mixed carefully.…”

“…Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RLFP) method was employed for genotype analysis of GH891 using MspI restriction enzyme, according to the procedure of Putra et al (2013) and Putra et al (2016). Briefly, 10 µL of PCR products were digested using 3U of MspI enzyme (Fermentas, Life Science) and incubated for 5 hours at 37 o C. PCR-RFLP products were run on 2% agarose gel…”

“…Nucleotide transversion of Guanine to Adenine changed (substitution) of amino acid (Leucine to Valine) that is recognized by AluI Restriction enzyme (Gordon et al, 1983). This single nucleotide polymorphism (SNP) resulted in two variant alleles: L and V. Allele L is more dominant than allele V in Holstein cattle (Mohammadabadi et al, 2010;Hadi et al, 2015), Zavot cattle/native Turkish cattle breeds (Akçay et al, 2015), Turkish cattle (Özkan ünal et al, 2015), Madura cattle and Pesisir cattle (Putra et al, 2016). Jakaria and Noor (2011) reported that monomorphic L of GH gene has been found in Bali cattle from Bali Islands.…”

Keragaman Baru pada Daerah Ujung Gen Hormon Pertumbuhan Sapi Pesisir Ternak Lokal Sumatera Barat

“…Polimorfisme di daerah exon dan intron memiliki potensi digunakan sebagai penanda genetik untuk perbaikan genetik populasi (Falaki et al, 1996). Telah banyak penelitian mencari hubungan antara varian gen ini dan sifat-sifat produksi (Tatsuda et al, 2008;Yardibi et al, 2009;Matsuhashi et al, 2010;Mohammadabadi et al, 2010;Maylinda, 2011;Sami, 2010;Sami et al, 2011;Moravčíková et al, 2012;Putra et al, 2015). Yurnalis et al (2013) telah meneliti keragam gen GH sapi Pesisir pada exon 4 dan sebagian intron 4 sedangkan penelitian tentang keragaman gen GH pada daerah ujung belum pernah dilakukan.…”

Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene

“…The GH gene polymorphism was intensively studied in many cattle breeds including local Indonesian cattle breeds (Paputungan et al 2012;Hartatik et al 2013;Putra et al 2014;Putra et al 2016) but limited reports for the Indonesian SO cattle GH gene. Up to present, information about the SO cattle GH gene polymorphism is limited only in the intron region (Agung et al 2017).…”