The pupose of this study was to identify genetic polymorphisms of bovine growth hormone gene exon 4, and intron 4 in local cattle breeds in West Sumatera Province of Indonesia. DNA was isolated from 60 blood samples and polymerase chain reaction (PCR) product of GH5 fragment (366 bp) were directly sequenced. Multiple alignments, including 60 bGH DNA sequences obtained by direct sequencing and bGH sequences from a public database (National Center for Biotechnology Information, acces number M57764), revealed 15 polymorphisms (five SNP, eight deletion, and two insertion). Eight deletions were
The Pesisir cattle are indigenous West Sumatera cattle. Their quality needs to be improved since they can live in local environment with sfficiently availablefeed resources. A research has been done to study the effects offeeding level andfattening period to the growth and the carcass characteristics of the Pesisir cattle. Thisresearchwasdesignedbyusingblockdesignwiththreeblocks. Thefirstfactorwastwolevelsoffeeding, i.e: Al : 50 % concentrate + 50 % ammoniated rice straw, A2 : 75 o% concentrate + 25 25 ammoniated rice straw. The second was time period offattening, i.e: B I : 3 months and 82 : 4 months. The results showed that there was no interaction between the level offeeding andfattening period to the growth, percentage of carcass, the backfat thickness and the rib eye area of the Pesisir cattle (P> 0.05). The higher of concentrate percentage, the higher the growth of the cattle, the thicker the backfat and the wider the rib eye area will be (P<0.05). Similarly, the longer thefattening, the higher the percentage of carcass, the thicker the backfat and tke wider rib eye area will b e. Ke1,w ords : P es is ir C at t I e, fe edin g I ev e I s, fatt enin g p er i o d, c arc es s Konsentrat Berbasis Bahan Lokal (Y'hastad, et al.)
ABSTRAKTujuan penelitian ini adalah untuk mengetahui potensi antibacterial bakteri asam laktat proteolitik dari bekasam sebagai biopreservatif daging sapi. Penelitian ini menggunakan 3 isolat bakteri asam laktat proteolitik dengan cara merendam daging dalam substrat antibakterial dari isolat BAL tersebut kemudian disimpan pada suhu dingin. Pengamatan utama pada penelitian ini adalah : (1) kualitas fisik daging, (2) kualitas mikrobiologi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan 3 kali ulangan. Faktor A adalah jenis BAL proteolitik yaitu a1 = Lactobacillus pentosus BS15, a2 = Lactobacillus plantarum 1 BS22 dan a3 = Lactobacillus plantarum 1 BL12 dan faktor B adalah penyimpanan daging pada suhu dingin selama 2, 4 dan 6 hari. Kualitas fisik daging menunjukkan bahwa nilai pH daging yang diberi substrat antibakterial Lactobacillus pentosus BS15 lebih rendah dibandingkan dengan Lactobacillus plantarum 1 BS22 dan Lactobacillus plantarum 1 BL12 sedangkan lama penyimpanan tidak berbeda. Daya ikat air daging yang diberi substrat antibakterial ketiga jenis bakteri tidak berbeda sedangkan lama penyimpanan 2 hari dan 4 hari lebih tinggi dibandingkan dengan 6 hari. Susut masak daging yang diberi substrat antibakterial Lactobacillus pentosus BS15 lebih kecil dari pada Lactobacillus plantarum 1 BS22 dan Lactobacillus plantarum 1 BL12, penyimpanan 2 hari dan 4 hari lebih besar susut masaknya dibandingkan 6 hari. Kualitas mikrobiologi menunjukkan bahwa total mikroba daging yang diberi substrat antibakterial Lactobacillus pentosus BS15 lebih tinggi dibandingkan dengan Lactobacillus plantarum 1 BS22 dan Lactobacillus plantarum 1 BL12 dan penyimpanan 2 hari lebih tinggi dibandingkan dengan 4 hari dan 6 hari. Total E. coli dan total S. aureus menunjukkan tidak ada perbedaan sedangkan lama penyimpanan 2 hari lebih tinggi dibandingkan dengan 4 hari dan 6 hari. Kesimpulan dari hasil penelitian ini penggunaan substrat antibakterial dari BAL proteolitik dan penyimpanan pada suhu dingin dapat mempengaruhi kualitas fisik dan mikrobiologis daging yang lebih baik.
This study was aimed to analyse the genetic polymorphism of Growth Hormone (GH) polymorphism of Simmental cattle using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and its relation to average daily gain. The research was conducted in the Padang Mangatas Breeding Centre, Limapuluh Kota district, West Sumatera Province and Biotechnology Laboratory of Faculty of Animal Husbandry, Andalas University. The research used 100 Simmental calves. DNA were isolation from blood sample using DNA purification Kit from Pomega. The PCR procedure was used to amplify 591-bp of bGH exon 1 (GH1) and 694-bp exon 2 (GH2). The PCR product were digested by restriction enzymes MspI and AluI. Digestion of 591-bp GH gen PCR product with enzyme restriction MspI reveal allele A(+) and B(-) with frequency 0.875 and 0.125 respectively and digestion with restriction enzyme AluI revealed allele C(+) and D(-) with frequency 0.95 and 0.05 respectively and. Digestion of 694-bp PCR product by MspI represent allele P(+) and Q(-) with frequency 0.88 and 0.12 respectively and digestion with AluI enzyme represent allele R(+) and S(-) with frequency 0.94 and 0.06 respectivly. The observed heterozygosity, effective allele numbers and polymorphism information content of GH1/MspI, GH2/MspI, GH1/AluI, and GH2-AluI were 0.11/0.1948, 0.04/0.1889, 0.00/0.0927, and 0.00/0,1096 respectively. Using GLM, there was no relation between these polymorphic and avarage daily gain of calve.
Dari 210 sampel sapi pesisisir umur 1,5 tahun, dipilih 60 sample berdasarkan berat badan yaitu 30 sampel dengan berat tinggi (125±9,02 kg) dan 30 sampel dengan berat badan rendah (65±6,09 kg). Sampel darah 60 ekor sapi diisolasi dan produk PCR dari fragmen gen GH (455 bp) disekuensing forward dan reserve.
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