BAZF, a member of the Bcl6 gene family, acts as a sequence-specific transcriptional repressor in association with Bcl6. However, the tissue expression pattern of BAZF differs from that of Bcl6, suggesting a Bcl6-independent function of BAZF. In order to examine the physiological function of BAZF, we generated BAZF-deficient mice and transgenic mice with BAZF-cDNA under the control of the lck proximal promoter (lck-BAZF). These mice were viable and no gross anatomical abnormalities were observed after birth. Since Bcl6 is a key molecule for the generation of memory T cells, we examined the function of T cells of these mice. We show here that cell proliferation of naive CD4 T cells, but not memory ones, of BAZF-deficient mice to anti-CD3 antibody stimulation was impaired. Conversely, cell proliferation of naive CD4 T cells, but not memory ones, of lck-BAZF mice was augmented. Since cell proliferation of naive CD4 T cells of lck-Bcl6 mice to anti-CD3 antibody stimulation was severely impaired, BAZF may attenuate the regulatory effect of Bcl6 on antigenic activation of naive CD4 T cells by Bcl6/BAZF heterodimer formation. These results suggest that BAZF is necessary for activation of naive T cells to antigenic stimulation.
Penelitian ini bertujuan untuk mengidentifikasi produktivitas sapi potong di Kabupaten Pesisir Selatan Provinsi Sumatera Barat dalam menghasilkan bibit sapi potong dan kelayakan daerah tersebut sebagai sumber sapi potong di Provinsi Sumatera Barat. Penelitian ini dilakukan dengan cara sensus dengan alat bantu kuesioner dengan metode Quota sampling digunakan untuk memilih tiga kecamatan yang memiliki populasi tinggi, sedang, dan rendah. Variabel yang diamati dalam penelitian ini adalah identitas peternak dan ternak. Data sekunder dari instansi terkait juga digunakan sebagai pendukung dalam penelitian ini. Perkembangan populasi ternak dianalisis dengan pendekatan teori pemuliabiakan ternak. Rata-rata kenaikan populasi ternak dianalisis menggunakan analisis time series (analisis runtut waktu). Hasil penelitian ini menunjukkan nilai efisiensi reproduksi (ER) sebesar 89,95% dan nilai natural increase (NI) sebesar 29,46% yang menunjukkan bahwa pertambahan populasi sapi tergolong sedang. Hasil penelitian ini juga menunjukkan bahwa secara umum populasi sapi potong di Kabupaten Pesisir Selatan masih cukup memenuhi kebutuhan daerah tersebut namun kekurangan ternak pejantan sebesar 12,32% dan populasi ternak betina melebihi kebutuhan sebesar 21,03% dengan nilai net replacement rate (NRR) jantan sebesar 87,68% dan NRR betina sebesar 121,03%. Secara rata-rata output sapi potong pada semua bangsa untuk sapi afkir jantan adalah sebesar 5,93%, betina sebesar 11,12% , sapi muda jantan sebesar 2,19%, dan sapi muda betina sebesar 1,01% dari total populasi sapi.
The aim of this study was to evaluate of genetic diversity of local cattle in Indonesia. We analyzed the polymorphism of mtDNA cytochrome b (cytb) gene using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Seventy-three Indonesian local cattle were used in the study that consisted of Madura cattle from Madura island (n = 15), Madura cattle from Sapudi island (n = 23), Limousin x Madura from Madura island (n = 5), Bali cattle from Kupang (n = 12), Aceh cattle from Aceh (n = 5), Pesisir cattle (n = 4), Bali cattle from West Sumatera (n = 1), and Java cattle from Pacitan (n = 8). The 464-bp fragment of cytochrome b (cyt b) gene was amplified using primers: forward primer of L14735 and reverse primer of H15149. The PCR product was digested using HinfI and TaqI restriction enzyme to identify the polymorphism of mtDNA cytochrome b (cytb) gene. The result showed that there was two haplotypes (A and B). Aceh and Pesisir cattle have 100% haplotype B meanwhile Bali cattle (from Kupang and West Sumatera) and Limura cattle have 100% haplotype A. Whereas, Madura cattle from Madura and Sapudi island and Java cattle have two different haplotypes (A and B). There was mt-DNA cytochrome b polymorphism of local cattle in Indonesia based on PCR-RFLP analysis method. Since the genetic variation in local cattle showed the differences in the specific area, it is necessary to control the cattle populations based on the species.
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