Associations Between Polymorphism of Growth Hormone Gene With Milk Production, Fat and Protein Content in Friesian Holstein Cattle

Abstract

ABSTRAKPenelitian ini bertujuan untuk menganalisis hubungan polimorfisme gen hormon pertumbuhan (leu/val) dan produksi susu pada sapi Friesian Holstein. Sampel penelitian berasal dari 62 ekor sapi yang diambil dari dua kelompok sapi Friesian Holstein (dari New Zealand=19 ekor and Australia=43 ekor). Polymerase Chain Reaction (PCR) yang dilanjutkan dengan Restriction Fragment Length Polymorphism (RFLP) menggunakan enzim restriksi AluI untuk menentukan genotip sapi. Genotip ditentukan berdasarkan target gen GH 211 bp yang terletak pada intron 4 dan exon 5 dari gen hormon pertumbuhan. Genotypes Leu/Leu (LL) pada kedua grup menunjukkan frekuensi yang lebih tinggi dibandingkan dengan genotip Leu/Val (LV). Sapi Friesian Holstein dari New Zealand memperlihatkan genotip LL dan LV sebesar 84% dan 16%. Sapi Friesian Holstein dari Australia memiliki genotip LL dan LV sebesar 79% and 21%. Hubungan antara polimorfisme Leu/Val pada gen hormon pertumbuhan terhadap produksi susu, kadar lemak dan protein tidak menunjukkan pengaruh yang nyata pada kedua grup. Berdasarkan pengelompokan asal sapi, hasil penelitian menunjukkan perbedaan yang nyata pada kadar lemak dan protein susu. Kadar lemak dan protein susu pada sapi FH yang diimpor dari Australia lebih tinggi dibandingkan dengan kadar lemak dan protein susu pada sapi FH yang diimpor dari New Zealand. Kata kunci: Friesian Holstein import, gen hormon pertumbuhan, produksi susu, kadar lemak dan protein ABSTRACTThe aim of the research was to determine the associations between polymorphism of the bovine growth hormone (GH) gene (Leu/Val) and milk production of Friesian Holstein Cattle. A total of 62 cows which consist of two Friesian Holstein cattle groups (from New Zealand=19 heads and Australia=43 heads) were used for the research. We performed the Polymerase Chain Reaction and followed by Restriction Fragment Length Polymorphism using AluI restriction enzyme. The genotype was observed base on the target gene GH 211 bp in the part of intron 4 and exon 5 of growth hormone gene. The frequencies of genotypes LL were found higher than genotype LV in both groups. Friesian Holstein cattle from New Zealand showed the genotype LL and LV as 84% and 16%, respectively. Friesian Holstein cattle from Australia show the genotype LL and LV as 79% and 21%, respectively. The association between Leu/Val polymorphism on milk production, fat and protein content in both groups did not show the significant effect. Base on two groups of the origin of cattle, the result showed the significant different on fat and protein content of milk. Fat and protein contents of milk were higher in breed of FH imported from Australia compared to those in breed of FH imported from New Zailand.

Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle

“…We used 100 samples (50 pairs of G 0 -G 1 ; G 1 was the daughter of G 0 ) that were still available on the farm and met the criteria of having individual first lactation milk yield and milk composition records. In fact, other studies on gene association and polymorphism used smaller sample sizes [16,19-21]. DNA from the samples was isolated from cow tail vein blood using a DNA Extraction Kit (gSYNC ™ , Geneaid, Taiwan) following the manufacturer’s protocol.…”

Association between GH (g.1456_1457insT), GHRH (g.4474 C>A), and Pit-1 (g.244G>A) Polymorphisms and Lactation Traits in Holstein Friesian Cattle

“…These results brought in a very high V allele (87.0%) than L allele (13.0%). By genotyping the GH|AluI locus of the two NZ and Australian imported HF kept at a Dairy Breeding Center in Central Java also reported a very common LL genotype compared to the LV genotype, i.e., 79-8% vs. 16-2%, respectively (Hartatik et al, 2015). For the Indonesian local Ongole Grade beef cattle was identified by Hartati et al (2019) that the genotype frequencies of the CC, CT, and TT genotypes were 1.1%, 18.8%, and 80.1%, respectively and the allele frequencies of C and T alleles were 10.5% and 89.5%, respectively.…”

Identification of Locus GH/Alui Polymorphisms of Kuantan and Pesisir Cattle

“…Bos indicus was group of cattle that have a dominant LL genotype compared to LV and VV genotypes (Biswas et al, 2003;Jakaria et al, 2007;Mu'in, 2008;Volkandari et al, 2013), as well as Bos sundaicus (Bali cattle) (Mu'in, 2008) and buffaloes (Biswas et al, 2003). Bos taurus such as Friesien Holstein (Biswas et al, 2003;Muin and Zurahmah, 2009;Hartatik et al, 2015), Karan Fries (Aruna et al, 2004), Brazilian Canchim (Silveira et al, 2008), Podolian cattle (Dario et al, 2005), and Limousin (Jakaria et al, 2009) were polymorphic, the frequency of L allele was not more than 0.99. Crosses Bos taurus X Bos indicus have produced polymorphic cattles at GH/AluI gene locus such as in Limousin X Madura (Volkandari et al, 2013), Simmental x PO (Mu'in, 2008) and Limousin x PO (Mu'in, 2008).…”