Point-of-care fluorescence immunoassay for prostate specific antigen

Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device

“…However, it is important to emphasise that the detection equipment used to quantify PSA in the immuno-pillar platform was an inverted fluorescence microscope, which is not portable, cost-effective or power-free, therefore not suitable for POC applications (Ikami et al, 2010). An immunochromatographic test integrated with a Laser Fluorescence Scanner was able to detect PSA in biological samples with a lower limit of detection of 0.02 ng/ml PSA in 15 min total assay time (Oh et al, 2009). This assay was able to obtain better sensitivity than the MCFphone in less time.…”

Fluorescence ELISA for sensitive detection of ochratoxin A based on glucose oxidase-mediated fluorescence quenching of CdTe QDs

“…Error bars were based on triplicate measurements. The LOD was calculated at 2.2 pg mL À1 according to the mean plus 3 times standard deviation (SD) [36], which is 15-fold lower than that of conventional HRP-based ELISA (34 pg mL À1 , Fig. S3).…”

Graphene oxide quantum dots@silver core–shell nanocrystals as turn-on fluorescent nanoprobe for ultrasensitive detection of prostate specific antigen

“…In recent years, fluorescent measurements have been extensively applied to environmental and biological analysis owing to its advantages, such as low-cost, operational simplicity, high sensitivity and real-time detection (Chen et al, , 2013Li et al 2014b). Due to these promising aspects, fluorescent immunosensors, as a kind of effective analytical technique, have been applied extensively in the quantitative detection of PSA (Oh et al, 2009). The "turn-off" switch system is commonly used for variety of fluorescence immunosensors.…”