Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device

Barbosa, Ana I.; Gehlot, Poonam; Sidapra, Kalpita; Edwards, Alexander D.; Reis, Nuno M.

doi:10.1016/j.bios.2015.03.006

Cited by 203 publications

(131 citation statements)

References 33 publications

(46 reference statements)

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“…The smartphones have been widely used to the development of POC analytical tools for different colorimetric quantitative analyses, including immunoassays, biochemical and healthcare experiments. [18][19][20][21][22] These applications are possible because the smartphones present the two functional components necessary for accomplishing optical detection: source (light-emitting diode, LED, white light from flash) and radiation detector (digital camera). 23 This camera exhibits resolution and sensitivity greater than those of conventional phone cameras.…”

mentioning

confidence: 99%

Smartphone for Point-of-Care Quantification of Protein by Bradford Assay

Camargo¹,

Vicentini²,

Gobbi³

et al. 2016

J. Braz. Chem. Soc.

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We address in this paper a powerful point-of-care platform to conduct the Bradford assay. Our method was based on smartphone for colorimetric quantification of total protein in human blood plasma, presenting low cost, simplicity, portability, autonomy and ability for remote transmission of the data. Other advantage concerns the high number of smartphone's users worldwide. This feature contributes for the application of the method by non-specialist people. The interferences from external light were successfully solved by illuminating the samples with a straightforward negatoscope. Our method generated a satisfactory exactness, with accuracy percentages ranging from 87.2 to 99.1%. Keywords: rapid test, albumin, human plasma, colorimetry, negatoscope IntroductionBradford assay is one of the most usual methods applied for determining protein in solution. [1][2][3][4] This method was proposed by Marion M. Bradford in 1976 5 and relies on the interaction of Comassie Brilliant Blue G-250 dye with the protein. After a short-time incubation, the anionic product of such reaction is optically monitored at 595 nm. 6 The advantages of the Bradford assay are that its routine is user-friendly, fast, reproducible, sensitive and selective to the target molecule. 7,8 Conversely, some pharmaceutical excipients interfere on the response of this approach even at low contents. In this case, the precipitation of the protein with sucrose showed to be an efficient alternative.9 Other bottleneck concerns the poor portability and relatively high cost (ca. US$ 10,000.00) of the spectrophotometer applied in the Bradford assays. These features undermine the compatibility of the method toward point-of-care (POC) analyses. 10POC platforms present low cost and high portability, robustness and simplicity. Accordingly, such technologies bypass the necessity for specialized personal and allow in situ measurements even at resource-limited environments, presenting key social and economic implications. Such methods have been recently used at diverse applications. [11][12][13][14][15][16] The POC technology is the major segment of the global in vitro diagnostics market. 11Nonetheless, despite the advantages of these methods, only a few commercial executions had been observed because of the production cost of such analytical platforms. Such drawback inhibits the creation of profitable businesses. 17We address herein a POC platform for performing the Bradford assay using smartphone-based optical detection. The smartphones have been widely used to the development of POC analytical tools for different colorimetric quantitative analyses, including immunoassays, biochemical and healthcare experiments. [18][19][20][21][22] These applications are possible because the smartphones present the two functional components necessary for accomplishing optical detection: source (light-emitting diode, LED, white light from flash) and radiation detector (digital camera). 23 This camera exhibits resolution and sensitivity greater than those of conventional phone camera...

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mentioning

confidence: 99%

Smartphone for Point-of-Care Quantification of Protein by Bradford Assay

Camargo¹,

Vicentini²,

Gobbi³

et al. 2016

J. Braz. Chem. Soc.

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“…There are some smartphone based devices that are not found in the app stores, but there is a clear displacement of lab equipment using mobile technology, such as in the areas of microplate reading (Berg et al, 2015;Christodouleas et al 2015;Fu et al, 2016), live cell imaging (Walzik et al, 2015), microfluidics for cancer detection (Barbosa et al, 2015), food allergen detection (Coskun et al, 2013), and immunochromatgraphic detection of bacteria (Rajendran et al, 2014).Nonetheless, there are areas where no changes are expected; and this is the work of dangerous chemical and pathogens (e.g. Biohazard Class 3 work).…”

Section: Resultsmentioning

confidence: 99%

The world of biomedical apps: their uses, limitations, and potential

Gan

Poon

2016

Sci Phone Appl Mob Devices

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Many significant biomedical discoveries of old were made in the private property of famous scientists e.g. Leeuwenhoek and Archimedes. Today, discoveries are made in brightly-lit, hi-tech, ergonomic buildings that house research institutes. While such development is advantageous in many aspects, the spatial restriction of research into well-organized structures may delay and limit the spontaneity necessary for discoveries. The smartphone and peripheral mobile devices have the potential to not only increase the productivity and mobility of biomedical research, but also restore some freedom from spatial constraints. One possible way this can occur is the development of a mobile biomedical lab that allows researchers to carry out core research processes 'on-the-go' without being spatially restrained within a building or availability of equipment. For this exciting prospect, we surveyed the Google and Apple app stores, discussing the limitations and the potential of this area. Based on the developments, it appears to be just a matter of time before the majority of biomedical labs processes and equipment become mobile, centred on the smartphone and peripheral devices.

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“…Examples included a microfluidic surface plasmon resonance (SPR) platform for bacterial pathogen detection 146 , a miniaturized nuclear magnetic resonance device (VNMR) for detecting biomarkers in urine 147 , a lateral electrophoretic flow platform paired with a CellScope mobile reader for antibody detection 148 , and an optofluidic flow analyzer to measure optical absorbance of RBCs 149 . Work has also been done to expand the types of analytes which can be detected in a clinic setting, such as the use of smartphone-coupled imaging for DNA strand imaging and length quantification 150 , and sensitive detection of prostate specific antigen (PSA) from whole-blood samples 151 . A portable, centrifugal sedimentation based immunoassay platform was developed for multiplexed detection of pathogenic bacteria ( E. coli , Listeria , Salmonella , and Shigella ) from a variety of sample matrices such as urine, blood and stool 152 .…”

Section: Recent Developments In Poc Diagnosticsmentioning

confidence: 99%