Platelet activation and residual activation potential during storage of hyperconcentrated platelet products in two different platelet additive solutions

Abstract

PLTs appear better preserved in vitro in PAS-27a than in T-Sol, and this suggests that storage of hcPCs in PAS-27a could be extended beyond 24 hours.

Usefulness of delta value of platelet parameters on ADVIA 120 for the functional reactivity of stored platelets

“…However, Cardigan et al (21) reported that the reactivity of platelets to stimuli such as physiological agonists, rather than the glycoprotein expression without agonists, might be a better predictor of in vivo platelet transfusion efficacy. Recently, several studies have reported on CD62P expression in agonist-stimulated platelets for the evaluation of platelet function in platelet products (8)(9)(10)(11)(12)(13)(14)(15). However, agonist-induced expression of CD62P by flow cytometry analysis is not easy to use routinely.…”

“…Currently, agonist-induced expression of CD62P, or other markers, has been investigated in several studies to determine platelet function in platelet products (8)(9)(10)(11)(12)(13)(14)(15), because the functional reactivity of platelets could be used to predict platelet transfusion efficacy. However, agonist-induced expression of CD62P by flow cytometry analysis has some disadvantages, including the expense of the equipment and reagents, and the difficulty of the associated procedures.…”

The clinical and biological impact of new pathogen inactivation technologies on platelet concentrates

“…Previous studies have shown that the degree of platelet activation, such as the activation measured by flow cytometry for specific markers, could have an impact on the functional capacity of platelets, leading to a decreased response in aggregation tests with different agonists [57][58][59].…”

In vitro evaluation of metabolic changes and residual platelet responsiveness in photochemical treated and gamma‐irradiated single‐donor platelet concentrates during long‐term storage

“…10,11 Previously, in vitro platelet (PLT) quality studies of PCT PLTs have been performed in buffycoat 4,5,12 and single-donor PCs 13-15 but, with the exception of one study of PLT function in PCT buffy-coat PCs, 4 no in vitro study of PLT function has yet compared PCT to conventional untreated and gamma-irradiated PCs. The initial PLT storage lesion induced during preparation is important for PLT quality during storage [16][17][18][19] as high levels of PLT activation decrease PLT response to agonist stimulation during storage [19][20][21][22][23] and may lower survival of transfused PLTs. [24][25][26][27] Reduced PLT function may be induced by PLT activation, plasma enzyme activities, loss of PLT responsiveness at the receptor level, decreased cell viability, pH changes, and inhibitory effects of plasma or cellular releasate in PCs.…”