In vitro evaluation of metabolic changes and residual platelet responsiveness in photochemical treated and gamma‐irradiated single‐donor platelet concentrates during long‐term storage

Apelseth, Torunn Oveland; Bruserud, Øystein; Wentzel‐Larsen, Tore; Bakken, Anne M.; Bjørsvik, Solfrid; Hervig, Tor

doi:10.1111/j.1537-2995.2007.01167.x

Cited by 79 publications

(110 citation statements)

References 77 publications

(134 reference statements)

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“…TRAP-6 stimulation induced an increase in P-selectin and PS on platelets adhering to fibrinogen until day 7 but did not have any additional effect on the expression of P-selectin or PS on platelets adhering to collagen beads. The reduced response to TRAP-6 at the end of storage (lower fibrinogen binding and lower expression of P-selectin on adherent platelets) is in accordance to other studies using aggregometry to assess platelet function [25][26][27].…”

Section: Discussionsupporting

confidence: 73%

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

et al. 2014

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The aim of the present study was to set up and evaluate a novel method for studies of platelet adhesion and activation in blood and platelet suspensions such as platelet concentrate (PC) samples using protein-coated polystyrene beads and flow cytometry. To demonstrate its usefulness, we studied PCs during storage. PCs were prepared by apheresis technique (N = 7).Metabolic variables and platelet function was measured on day 1, 5, 7 and 12 of storage.Spontaneous and TRAP-6-induced adhesion to fibrinogen-and collagen-coated beads was analysed by flow cytometry. P-selectin and phosphatidyl serine (PS) expression was assessed on platelets bound to beads as well as on non-adherent platelets. Platelet adhesion to fibrinogen beads had increased by day 12 and adhesion to collagen beads at day 7 of storage (p < 0.05). TRAP-6 stimulation significantly increased the platelet adhesion to fibrinogen beads (p < 0.05) as well as the P-selectin and PS exposure on platelets bound to beads (p < 0.01) during the first 7 days of storage, but by day 12, significant changes were no longer induced by TRAP-6 stimulation.We demonstrate that our adhesion assay using protein-coated polystyrene beads can be used to assess the adhesion properties of platelets during storage without the addition of red blood cells. Therefore it may offer a useful tool for future studies of platelet adhesive capacity in transfusion medicine and other settings.3

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Section: Discussionsupporting

confidence: 73%

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

et al. 2014

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“…The most likely explanation for the discrepancies between our results and the study by Stivala et al 1 and others [4][5][6] is the fact that we isolated platelets from the storage milieu in order to explore their intrinsic functional properties, independently of the storage milieu which may have an inhibitory-yet-reversible effect on platelet responsiveness. We also looked at the reactivity of platelets in their storage milieu and, indeed, found there was already inhibition of platelet aggregation in response to ADP on Day 1.5, and to collagen on Day 6.5, which was, however, similar between the untreated and the IBS-PCs 2 (see Online Supplementary Figure S2B).…”

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confidence: 54%

Amotosalen/UVA pathogen inactivation technology reduces platelet activability, induces apoptosis and accelerates clearance

2017

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Amotosalen/UVA pathogen inactivation technology reduces platelet activability, induces apoptosis and accelerates clearanceWith interest we read the paper by Stivala et al., 1 recently published in Haematologica, evaluating the structural and functional consequences induced by Amotosalen/UVA treatment using the Intercept Blood System (IBS) on platelets from apheresis platelet concentrates (PCs) during storage. This study provides results of early diminished platelet function in IBS-treated PCs as compared to conventional PCs, i.e., reduced aggregation response to collagen or thrombin and adhesion to collagen or vWF under flow, increased platelet apoptosis, MAPK p38 activation, and glycoprotein Ibα (GPIbα) shedding and enhanced clearance from the circulation of mice.These results strikingly contrast with our previous published study 2 which evaluated the impact of IBS on platelets isolated from buffy coat PCs, washed and suspended in Tyrode's buffer. Surprisingly, Stivala et al.

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“…However, Cardigan et al (21) reported that the reactivity of platelets to stimuli such as physiological agonists, rather than the glycoprotein expression without agonists, might be a better predictor of in vivo platelet transfusion efficacy. Recently, several studies have reported on CD62P expression in agonist-stimulated platelets for the evaluation of platelet function in platelet products (8)(9)(10)(11)(12)(13)(14)(15). However, agonist-induced expression of CD62P by flow cytometry analysis is not easy to use routinely.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, agonist-induced expression of CD62P, or other markers, has been investigated in several studies to determine platelet function in platelet products (8)(9)(10)(11)(12)(13)(14)(15), because the functional reactivity of platelets could be used to predict platelet transfusion efficacy. However, agonist-induced expression of CD62P by flow cytometry analysis has some disadvantages, including the expense of the equipment and reagents, and the difficulty of the associated procedures.…”

Section: Introductionmentioning

confidence: 99%

Usefulness of delta value of platelet parameters on ADVIA 120 for the functional reactivity of stored platelets

Park

Lim

Cho³

2010

Clinical Laboratory Analysis

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An agonist-induced expression of CD62P by flow cytometry analysis for evaluating platelet functional reactivity has some disadvantages. We investigated the usefulness of platelet parameters by ADVIA 120 to predict an agonist-induced expression of CD62P in stored platelets. The CD62P expression by flow cytometry and the platelet parameters by ADVIA 120 were studied in samples from 27 platelet pheresis products. Delta (D) values were calculated as the degree of change of the platelet parameters studied with or without adenosine 5 0 -diphosphate sodium (ADP) stimulation. The CD62P expression of the ADP-activated platelets were correlated with the Dplatelet count (r 5 0.517) in the short-term storage group (within 10 hr from preparation), with the platelet component distribution width (PCDW) without ADP (r 5 À0.744) and the DPCDW (r 5 À0.755) in the long-term storage group (after 10 hr from preparation). Therefore, the delta values of platelet parameters on ADVIA 120 analysis in platelets between with and without ADP stimulation could be useful as a simple predictor for the functional reactivity of stored platelets.

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In vitro evaluation of metabolic changes and residual platelet responsiveness in photochemical treated and gamma‐irradiated single‐donor platelet concentrates during long‐term storage

Cited by 79 publications

References 77 publications

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Amotosalen/UVA pathogen inactivation technology reduces platelet activability, induces apoptosis and accelerates clearance

Usefulness of delta value of platelet parameters on ADVIA 120 for the functional reactivity of stored platelets

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