Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Tynngård, Nahreen; Wallstedt, Maria; Södergren, Anna L.; Faxälv, Lars; Ramström, Sofia

doi:10.3109/09537104.2014.891728

Cited by 18 publications

(16 citation statements)

References 34 publications

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“…In addition, PS exposure, another marker of platelet activation and apoptosis [19], was significantly increased in NF-PC group, which is in agreement with previous studies [30,31]. However, the significance of this process remains to be clarified [9]. We also evaluated MMP in the two groups, as platelet apoptosis was previously associated with the loss of MMP and intrinsic apoptotic pathway [32].…”

Section: Discussionsupporting

confidence: 87%

“…CD42b is a receptor for von Willebrand factor (VWF); the binding of CD42b to VWF initiates platelet deposition, and the complex is cleaved upon platelet activation [26]. In some studies, stabile CD42b expression was demonstrated during platelet storage [9,27], while in other studies, decreased CD42b expression was observed [28,29]. These differences in CD42b expression in platelets may be due to the differences in storage time, e.g., in our study the storage time was 5 days, while in other studies it was 12 and 14 days [28,29].…”

Section: Discussionmentioning

confidence: 99%

“…Different methods for pathogen reduction and leukocyte depletion in PCs produce platelets that vary greatly in their quality. Thus, a proper and early detection of PSL in platelets remains one of the most important aspects of platelet transfusion [9]. In this study, we evaluated the efficacy of an in-line filtration method for obtaining PCs compared to the standard method.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of platelet activation during storage in leukocyte-depleted platelet concentrates

Vučić

Stanojkovic²,

Antić³

et al. 2017

Bosn J of Basic Med Sci

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Structural and functional changes in platelets during storage can lead to the loss of platelet reactivity and response. Our aim was to evaluate leukocyte-depleted platelet concentrates on storage days 0, 3 and 5, obtained by in-line filtration. In non-filtered platelet concentrates (NF-PC) group, 180 whole blood units were collected with quadruple blood bags and then compared to another group of 180 whole blood units (leukocyte-depleted platelet concentrates [LD-PC]), collected in Imuflex Whole Blood Filter Saving Platelets (WB-SP) bags with an integrated leukoreduction filter, with regard to the platelet quality and characteristics. The efficacy of the two techniques for platelet concentrate preparation was evaluated by white blood cell (WBC) and platelet count on day 0. The partial pressure of oxygen (pO2), pH, platelets positive for P-selectin (CD62P), CD63, cluster of differentiation 42b (CD42b), phosphatidylserine (PS), and mitochondrial membrane potential (MMP) were analyzed during the storage in both groups. A significantly lower WBC count and higher platelet count was observed in LD-PC compared to NF-PC group, indicating the overall efficacy of the first technique. During the 5-day storage, pH and pO2 decreased in both groups. In LD-PC group, higher pH, increased pO2 and decreased platelet surface expression of CD62P, CD63 and PS were observed compared to NF-PC group. In both groups, the percentage of CD42b positive platelets and MMP did not change significantly during the 5-day period. The assessment of different markers of platelet activation may be an effective tool in evaluating the quality of platelets during storage. A better understanding of platelet activation may provide new insights for developing a novel therapeutic approach in the manipulation of platelet aggregation.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of platelet activation during storage in leukocyte-depleted platelet concentrates

Vučić

Stanojkovic²,

Antić³

et al. 2017

Bosn J of Basic Med Sci

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“…Other options to study platelet activation using flow cytometry include the agonist-induced formation of platelet microaggregates, detectable by residual platelet counting, 101 the adhesion of platelets to protein-coated polystyrene beads, 102 and the formation of platelet-leukocyte aggregates 103 in circulation or upon platelet activation. [104][105][106] In studies of platelet-leukocyte aggregate formation, coincidence issues need to be taken into account 107 and distinguished from true aggregates.…”

Section: Alternative Ways To Test Platelet Function By Flow Cytometrymentioning

confidence: 99%

Platelet Function Determined by Flow Cytometry: New Perspectives?

Ramström

Södergren

Tynngård

et al. 2016

Semin Thromb Hemost

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Flow cytometry enables studies of a number of different aspects of platelet function in response to a variety of platelet agonists. This can be done using only a small volume of whole blood, and also in blood with low platelet counts. These properties, together with the increasing number of flow cytometers available in hospitals worldwide makes flow cytometry an interesting option for laboratories interested in studies of platelet function in different clinical settings. This review focuses on practical issues regarding the use of flow cytometry for platelet function testing. It provides an overview of available activation markers, platelet agonists and experimental setup issues. The review summarizes previous experience and factors important to consider in order to perform high quality platelet function testing by flow cytometry. It also discusses its current use, and possibilities and challenges for future use of flow cytometry in clinical settings.

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“…The selection of blood from animal species (e.g., pig and sheep) instead of human blood has to be avoided, particularly in view of the species‐dependent platelet function . In blood products, such as frozen plasma or platelet concentrates, proteins (e.g., factors V and VIII, or cytokines) and cells (e.g., platelet adhesion and aggregation) undergo functional alterations due to the processing (e.g., cooling and freezing), storage (e.g., storage lesion of cells), and stabilization . Thus, the use of blood products should be avoided since it very likely lead to an underestimation of the thrombogenicity of the tested materials.…”

Section: Prerequisites For a Reproducible In Vitro Testingmentioning

confidence: 99%

In Vitro Thrombogenicity Testing of Biomaterials

Braune

Latour

Reinthaler

et al. 2019

Adv Healthcare Materials

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reliable, and reproducible. [20][21][22] This is, not least, important for realizing interstudy comparisons of the thrombogenicity of different materials. According, e.g., to the regulation (EU) 2017/745 of the European parliament and of the council on medical devices, in vitro and in vivo tests are a mandatory parts within the preclinical product verification and validation (see Annex II: Technical documentation, 6.1 Preclinical and clinical data). [23] Beyond the results of these tests, also detailed information concerning the test design, respective protocols and data analysis are obligatory to meet the requirements of the regulation, particularly those formulated in the ISO standard 10993 Part 4. [24,25] Since the regulatory agencies provide recommendations rather than protocols or standard operation procedures, accepted standardizations regarding the preanalytical handling of the blood, test conditions, test parameters, reference materials, static or dynamic conditions, flow conditions, and finally, which type of cells and donors should be included is lacking. Also, standard operating procedures how the testing should be performed are lacking. [9] This has led to a situation that laboratories perform in vitro assays under substantially different test conditions, including the preanalytical characterization of blood samples, the application of blood from different species, varying anticoagulation and stabilization or storage times as well as test setups. Taking these variations into account, interlaboratory or interstudy comparisons are impossible. [8,[26][27][28] Here, we review described test methods and procedures for the assessment of the thrombogenicity of materials, prerequisites for a reproducible in vitro testing, applied test system and test parameters for the characterization of the interaction of blood components/cells and the implant. We further review and outline recent approaches that may support realizing reliable, reproducible, and laboratory independent tests.

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Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Cited by 18 publications

References 34 publications

Evaluation of platelet activation during storage in leukocyte-depleted platelet concentrates

Evaluation of platelet activation during storage in leukocyte-depleted platelet concentrates

Platelet Function Determined by Flow Cytometry: New Perspectives?

In Vitro Thrombogenicity Testing of Biomaterials

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