Usefulness of delta value of platelet parameters on ADVIA 120 for the functional reactivity of stored platelets

Abstract

An agonist-induced expression of CD62P by flow cytometry analysis for evaluating platelet functional reactivity has some disadvantages. We investigated the usefulness of platelet parameters by ADVIA 120 to predict an agonist-induced expression of CD62P in stored platelets. The CD62P expression by flow cytometry and the platelet parameters by ADVIA 120 were studied in samples from 27 platelet pheresis products. Delta (D) values were calculated as the degree of change of the platelet parameters studied with or without adenosine 5 0 -diphosphate sodium (ADP) stimulation. The CD62P expression of the ADP-activated platelets were correlated with the Dplatelet count (r 5 0.517) in the short-term storage group (within 10 hr from preparation), with the platelet component distribution width (PCDW) without ADP (r 5 À0.744) and the DPCDW (r 5 À0.755) in the long-term storage group (after 10 hr from preparation). Therefore, the delta values of platelet parameters on ADVIA 120 analysis in platelets between with and without ADP stimulation could be useful as a simple predictor for the functional reactivity of stored platelets.

Automated preparation of whole blood–derived platelets suspended in two different platelet additive solutions and stored for 7 days

“…Several studies have recently demonstrated that MPC values are inversely correlated with PLT membrane activation and subsequent P‐selectin expression . In fact, the MPC variable has been used as an indicator of PLT activation in different conditions and physiologic states,28‐31 as well as stored PCs . In the present study, we observed that MPC decreased during 7‐day PLT storage indicating PLT activation in the three arms of the study.…”

“…27 In fact, the MPC variable has been used as an indicator of PLT activation in different conditions and physiologic states, [28][29][30][31] as well as stored PCs. 25,32,33 In the present study, we observed that MPC decreased during 7-day PLT storage indicating PLT activation in the three arms of the study. However, higher MPC indicating lower PLT activation was seen in PCs prepared with the Atreus 3C and suspended in PAS-IIIM when compared with the other two arms of the study.…”

Comparison of platelet activation in platelet concentrates measured by flow cytometry or ADVIA 2120

“…This study showed that PCDW correlated with CD62P expression during 5 days of storage, but there was no correlation between MPC and CD62P. Other investigators [10] showed a negative correlation between CD62P and the change in PCDW (from the time zero value), in stored apheresis platelets anticoagulated with Acid Citrate Dextrose (ACD) and activated with adenosine diphosphate (ADP). However, neither of these studies incorporated the addition of extra anticoagulant to the platelet concentrate samples.…”

Influence of cryopreservation and mechanical stimulation on equine Autologous Conditioned Plasma (ACP®)

“…Decrease in MPC and increase in MPV are reliable indicators of platelet activation, because activation of these cells is associated to cell degranulation and cell swelling, respectively (24). Mean MPC significantly decreased in a similar manner in ACP® after both storage processes.…”

“…Platelets are prone to activation, which induces an increase in their size, shape alterations and a decrease in their cellular density (36,37). Their activation level can be objectively measured with laser flow cytometry to determine their volume (mean platelet volume, MPV) and refractive index (mean platelet component, MPC) (24). Exogenous platelet activation techniques, such as addition of collagen, thrombin, calcium chloride or combinations thereof (25,37,41), show dose-dependent effects (18), but they might also induce undesirable inflammatory reactions (38).…”