Pichia pastoris expressing recombinant tilapia growth hormone accelerates the growth of tilapia

Acosta, Jannel; Morales, Reynold; Morales, Antonio; Alonso, Miriam Crespo; Estrada, Mario Pablo

doi:10.1007/s10529-007-9502-7

Cited by 33 publications

(25 citation statements)

References 11 publications

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“…Immersion of fish juvenile in the medium containing rGH enhances the growth, such as 43% in coho salmon and about 60% in chum salmon (Moriyama & Kawauchi, 1990). In addition, immersion of Nile tilapia larvae in the water containing rGH-produced Pichia pastoris can drastically enhanced the growth; 171% (Acosta et al, 2007). In our ongoing study, immersion of giant gouramy larvae in the water containing giant gouramy rGH (Og-mGH) improved the body weight; almost 2-fold higher compared to the control.…”

Section: Discussionmentioning

confidence: 99%

Production and Bioactivity Potential of Three Recombinant Growth Hormones of Farmed Fish

Alimuddin

Lesmana

Sudrajat

et al. 2010

Indonesian Aquaculture Journal

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This study was aimed to produce recombinant growth hormone (rGH) from giant grouper (Epinephelus lanceolatus), giant gouramy (Osphronemus gouramy) and common carp (Cyprinus carpio) and compare their bioactivity potential by means of inducing the growth hormone of juvenile Nile tilapia (Oreochromis niloticus) as the model. DNA fragment encoding mature GH protein of giant grouper (El-mGH), giant gouramy (Og-mGH) and common carp (Cc-mGH) was amplified by PCR method. The purified PCR products were ligated to pCold-1 to generate pCold/El-mGH, pCold/OgmGH, and pCold/Cc-mGH protein expression vector, respectively. Each of the expression vectors was transformed into the Escherichia coli BL21. E. coli BL21 was cultured using 2xYT medium and protein production was induced by cold shock at 15±1 o C for overnight. The inclusion bodies of E. coli transformants containing protein expression vector were isolated by sonication method, and rGH production was analyzed by SDS-PAGE. Juvenile of Nile tilapia of average body weight of 12.41±3.28 g was intramuscularly injected once a week for 4 weeks with 1 μg inclusion body containing rGH per gram fish body weight. The result showed that rGH in molecular weight of about 25 kDa was obtained. Fish injected with rGH of El-mGH, Cc-mGH and Og-mGH grew 20.94%, 18.09%, and 16.99% faster, respectively, compared with the control. This result indicated that the three rGH produced in E. coli possessed biological activity when tested on Nile tilapia and further research is needed to find its effect on the growth of other aquaculture fish species. KEYWORDS: bioactivity, recombinant protein, growth hormone, Nile tilapiaProduction and bioactivity potential of three recombinant ... (Alimuddin)

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Section: Discussionmentioning

confidence: 99%

Production and Bioactivity Potential of Three Recombinant Growth Hormones of Farmed Fish

Alimuddin

Lesmana

Sudrajat

et al. 2010

Indonesian Aquaculture Journal

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“…Pemberian rGH yang berbeda pada ikan nila melalui teknik penyuntikan meningkatkan bobot 20,94% (rGH ikan kerapu kertang), 18,09% (rGH ikan mas), dan 16,99% (rGH ikan gurami) (Alimuddin et al, 2010). Acosta et al (2007) melaporkan perendaman hormon pertumbuhan dapat meningkatkan bobot ikan nila sebesar 171%. Perendaman hormon pertumbuhan terhadap ikan gurami juga dapat meningkatkan bobot ikan gurami sebesar 75% (Alimuddin et al, 2011).…”

Section: Abstrakunclassified

Effectivity of thyroxine and recombinant growth hormone on the growth of Siam-catfish larvae

Sudrajat

Muttaqin

Ali

2014

Jurnal Akuakultur Indonesia

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KKP. Ikan ini sangat digemari masyarakat karena memiliki cita rasa yang khas, kandungan protein tinggi, dan dapat dikonsumsi segar serta bahan olahan. Berdasarkan informasi pasar tahun 2012 bahwa nilai ekonomis ikan patin relatif tinggi, harga telur Rp5 sampai Rp7/butir, harga benih 0,75 inci Rp60/ekor, dan harga benih ukuran 1 PENDAHULUAN ABSTRACTCatfish hatchery requires technology and engineering to maximize the development and growth of fish. In this research the hormone thyroxine (T4), recombinant growth hormone (G) and mix of thyroxine and growth hormones (GT) were administered by immersion to enhance the development and growth of stripped catfish larvae. Research was using completely randomized design, with four treatments and five replications; A, control; B, thyroxine hormone ( The size of liver cell and cytoplasm of treated larvae were larger than the control. Behavior of fish in the treatments B and C showed more active than the treatments A and D. Thus, combination of thyroxine and recombinant growth hormone treatment (C) has an efficient of yolk utilization, and higher in development and growth of stripped catfish larvae.Keywords: thyroxine, growth hormone, yolk absorption, growth, stripped catfish ABSTRAKPembenihan ikan patin membutuhkan teknologi dan rekayasa untuk memaksimumkan perkembangan, dan pertumbuhan ikan. Pada penelitian ini dilakukan pemberian hormon tiroksin (T4), hormon pertumbuhan rekombinan (G) serta hormon gabungan antara hormon tiroksin dan hormon pertumbuhan (GT) melalui perendaman untuk memacu perkembangan dan pertumbuhan larva ikan patin siam. Penelitian menggunakan rancangan acak lengkap, dengan empat perlakuan dan lima kali ulangan; A, kontrol; B, hormon tiroksin (T4) 0,1 mg/L; C, T4 dan G (GT) (0,1 mg/L dan 10 mg/L); dan D, G 10 mg/L. Perendaman dilakukan selama satu jam. Hasil penelitian menunjukkan bahwa laju penyerapan kuning telur pada jam ke-16 setelah perendaman lebih cepat (P<0,05) pada perlakuan B (96,98%) dibandingkan dengan perlakuan A (18,54%), C (20,59%), dan D (32,90%). Pertumbuhan larva yang diberi perlakuan B (24,85 mm) dan C (24,00 mm) lebih tinggi (P<0,05) dibandingkan dengan perlakuan A (21,71 mm) dan D (23,18 mm). Tingkat kelangsungan hidup antarperlakuan tidak berbeda (P>0,05). Ukuran sel dan sitoplasma hati ikan perlakuan relatif lebih besar dibandingkan kontrol. Tingkah laku ikan pada perlakuan B dan C lebih aktif dibandingkan perlakuan A dan D. Dengan demikian kombinasi hormon tiroksin dan hormon pertumbuhan rekombinan secara bersama (C) memiliki efisiensi pemanfaatan kuning telur, perkembangan, dan pertumbuhan lebih tinggi pada larva ikan patin.Kata kunci: tiroksin, hormon pertumbuhan, penyerapan kuning telur, pertumbuhan, ikan patin siam

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“…Produksi rGH menggunakan bioreaktor dilakukan setelah diketahui bahwa pemberian hormon pertumbuhan alami dapat memacu pertumbuhan, dan gen penyandinya berhasil diisolasi. Berbagai rGH ikan telah berhasil diproduksi seperti rGH ikan salmon (Sekine et al, 1985), rGH ikan flounder (Jeh et al, 1998), rGH orange-spotted grouper (Li et al, 2003); rGH ikan patin siam (Poen, 2009), rGH ikan mas "rCcGH", rGH ikan gurame "rOgGH" dan rGH ikan kerapu kertang "rElGH" (Alimuddin et al, 2010).…”

Section: Pendahuluanunclassified

RESPONS PERTUMBUHAN DAN EKSPRESI GEN UDANG VANAME, Litopenaeus vannamei SETELAH DIRENDAM DALAM LARUTAN HORMON PERTUMBUHAN REKOMBINAN IKAN KERAPU KERTANG

Subaidah¹,

Carman

Sumantadinata

et al. 2012

Jurnal Riset Akuakultur

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ABSTRAKPertumbuhan ikan dapat ditingkatkan menggunakan hormon pertumbuhan rekombinan. Penelitian ini bertujuan mengkaji respons pertumbuhan dan ekspresi gen udang vaname, Litopenaeus vannamei setelah direndam dengan hormon pertumbuhan rekombinan ikan kerapu kertang, Epinephelus lanceolatus (rElGH). Pada percobaan pertama, post larva stadia 2 (PL-2) sebanyak 1.500 ekor direndam selama 1 jam dalam 1 liter air laut yang mengandung rElGH lima dosis berbeda, yaitu 150; 15; 1,5; 0,15; dan 0,015 mg/L dan bovine serum albumin 0,01%. Setiap perlakuan diulang 3 kali. Perendaman dilakukan dalam kantong plastik ditambah oksigen (volume air : oksigen = 1:5). Udang dipelihara dalam akuarium volume 60 liter dengan kepadatan 25 ekor/liter sampai PL-14. Hasil penelitian menunjukkan bahwa dosis 15 mg/L memberikan peningkatan bobot badan, panjang badan, dan sintasan tertinggi (P<0,05) masingmasing sebesar 37,77%; 12,75%; dan 9,45% dibandingkan dengan kontrol. Ekspresi mRNA single insulin binding domain (SIBD) pada PL-14 yang dianalisis dengan realtime PCR menunjukkan kenaikan sebesar 3,3 kali pada udang yang direndam rElGH dibandingkan dengan kontrol, dan dapat dinyatakan bahwa SIBD berperan penting dalam induksi pertumbuhan. Tingkat ekspresi moult inhibiting hormone meningkat sekitar 13%, sedangkan ekspresi cyclopilin A pada udang yang direndam rElGH sama dengan kontrol. Pada percobaan kedua, perendaman PL-2 dalam larutan rElGH dosis 15 mg/L dengan lama waktu 3 jam meningkatkan bobot badan sebesar 62,18% lebih tinggi daripada perendaman 1 jam. Dengan demikian, perendaman udang dalam larutan rElGH meningkatkan pertumbuhan dan ekspresi gen SIBD, dan metode ini dapat berguna dalam peningkatan produksi budidaya.

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Pichia pastoris expressing recombinant tilapia growth hormone accelerates the growth of tilapia

Cited by 33 publications

References 11 publications

Production and Bioactivity Potential of Three Recombinant Growth Hormones of Farmed Fish

Production and Bioactivity Potential of Three Recombinant Growth Hormones of Farmed Fish

Effectivity of thyroxine and recombinant growth hormone on the growth of Siam-catfish larvae

RESPONS PERTUMBUHAN DAN EKSPRESI GEN UDANG VANAME, Litopenaeus vannamei SETELAH DIRENDAM DALAM LARUTAN HORMON PERTUMBUHAN REKOMBINAN IKAN KERAPU KERTANG

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