Production and Bioactivity Potential of Three Recombinant Growth Hormones of Farmed Fish

Abstract

This study was aimed to produce recombinant growth hormone (rGH) from giant grouper (Epinephelus lanceolatus), giant gouramy (Osphronemus gouramy) and common carp (Cyprinus carpio) and compare their bioactivity potential by means of inducing the growth hormone of juvenile Nile tilapia (Oreochromis niloticus) as the model. DNA fragment encoding mature GH protein of giant grouper (El-mGH), giant gouramy (Og-mGH) and common carp (Cc-mGH) was amplified by PCR method. The purified PCR products were ligated to pCold-1 to generate pCold/El-mGH, pCold/OgmGH, and pCold/Cc-mGH protein expression vector, respectively. Each of the expression vectors was transformed into the Escherichia coli BL21. E. coli BL21 was cultured using 2xYT medium and protein production was induced by cold shock at 15±1 o C for overnight. The inclusion bodies of E. coli transformants containing protein expression vector were isolated by sonication method, and rGH production was analyzed by SDS-PAGE. Juvenile of Nile tilapia of average body weight of 12.41±3.28 g was intramuscularly injected once a week for 4 weeks with 1 μg inclusion body containing rGH per gram fish body weight. The result showed that rGH in molecular weight of about 25 kDa was obtained. Fish injected with rGH of El-mGH, Cc-mGH and Og-mGH grew 20.94%, 18.09%, and 16.99% faster, respectively, compared with the control. This result indicated that the three rGH produced in E. coli possessed biological activity when tested on Nile tilapia and further research is needed to find its effect on the growth of other aquaculture fish species. KEYWORDS: bioactivity, recombinant protein, growth hormone, Nile tilapiaProduction and bioactivity potential of three recombinant ... (Alimuddin)

Growth in Nursery and Grow-Out Phases of White Shrimp After Immersed in Recombinant Giant Grouper Growth Hormone

“…Salah satu teknologi yang dapat digunakan untuk meningkatkan laju pertumbuhan adalah dengan menggunakan hormon pertumbuhan rekombinan (recombinant growth hormone, rGH). Pemberian rGH telah diuji dan dilaporkan secara signifikan dapat meningkatkan laju pertumbuhan pada ikan, baik pada spesies yang sama dengan sumber gen yang digunakan untuk membuat rGH , maupun berbeda spesies (Alimuddin et al, 2010;Handoyo et al, 2012). Bahkan hormon pertumbuhan dari mamalia juga telah teruji efektivitasnya dalam menginduksi pertumbuhan ikan (Silverstein et al, 2000).…”

“…Suri Tani Pemuka, Banyuwangi, Jawa Timur. Hormon pertumbuhan rekombinan yang digunakan adalah hormon pertumbuhan rekombinan ikan kerapu kertang (rElGH) yang dikembangkan oleh Alimuddin et al (2010) dan diproduksi oleh Balai Besar Perikanan Budidaya Air Tawar Sukabumi, Jawa Barat. Dosis perendaman yang digunakan pada tahap ini adalah 15 mg/L (Subaidah et al, 2012).…”

Growth Performance and Histological Appearance of the Humpback Grouper Juvenile (Cromileptesaltivelis) After Treated With Recombinant Growth Hormone

“…Escherichia colistrain BL21 harboring pCold-ElGHprotein expression vectorwas used as bioreactor for producing rElGH.Bacteria culture, extractionand verification of protein rElGH were done according to Alimuddin et al (2010).To protect rElGH from proteolitic digestive enzyme, the protein was coated by hydroxypropylmethyl cellulose phthalate (HP55, Shinetsu, Japan) as described by Moriyama et al(1993) before it mixed with commercial diet (CD).…”

Effectivity of thyroxine and recombinant growth hormone on the growth of Siam-catfish larvae

“…Alimuddin et al (2010) telah berhasil membuat protein hormon pertumbuhan rekombinan (rGH) ikan gurami, ikan mas, dan ikan kerapu kertang. Pemberian rGH yang berbeda pada ikan nila melalui teknik penyuntikan meningkatkan bobot 20,94% (rGH ikan kerapu kertang), 18,09% (rGH ikan mas), dan 16,99% (rGH ikan gurami) (Alimuddin et al, 2010). Acosta et al (2007) melaporkan perendaman hormon pertumbuhan dapat meningkatkan bobot ikan nila sebesar 171%.…”