ABSTRAKPertumbuhan ikan dapat ditingkatkan menggunakan hormon pertumbuhan rekombinan. Penelitian ini bertujuan mengkaji respons pertumbuhan dan ekspresi gen udang vaname, Litopenaeus vannamei setelah direndam dengan hormon pertumbuhan rekombinan ikan kerapu kertang, Epinephelus lanceolatus (rElGH). Pada percobaan pertama, post larva stadia 2 (PL-2) sebanyak 1.500 ekor direndam selama 1 jam dalam 1 liter air laut yang mengandung rElGH lima dosis berbeda, yaitu 150; 15; 1,5; 0,15; dan 0,015 mg/L dan bovine serum albumin 0,01%. Setiap perlakuan diulang 3 kali. Perendaman dilakukan dalam kantong plastik ditambah oksigen (volume air : oksigen = 1:5). Udang dipelihara dalam akuarium volume 60 liter dengan kepadatan 25 ekor/liter sampai PL-14. Hasil penelitian menunjukkan bahwa dosis 15 mg/L memberikan peningkatan bobot badan, panjang badan, dan sintasan tertinggi (P<0,05) masingmasing sebesar 37,77%; 12,75%; dan 9,45% dibandingkan dengan kontrol. Ekspresi mRNA single insulin binding domain (SIBD) pada PL-14 yang dianalisis dengan realtime PCR menunjukkan kenaikan sebesar 3,3 kali pada udang yang direndam rElGH dibandingkan dengan kontrol, dan dapat dinyatakan bahwa SIBD berperan penting dalam induksi pertumbuhan. Tingkat ekspresi moult inhibiting hormone meningkat sekitar 13%, sedangkan ekspresi cyclopilin A pada udang yang direndam rElGH sama dengan kontrol. Pada percobaan kedua, perendaman PL-2 dalam larutan rElGH dosis 15 mg/L dengan lama waktu 3 jam meningkatkan bobot badan sebesar 62,18% lebih tinggi daripada perendaman 1 jam. Dengan demikian, perendaman udang dalam larutan rElGH meningkatkan pertumbuhan dan ekspresi gen SIBD, dan metode ini dapat berguna dalam peningkatan produksi budidaya.
<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">The important role in determining the productivity of shrimp was the quality and quantity of shrimp sperm. The decreasing of hatching rate was predicted as the effect of the decreasing quality of sperm. It then could influence the number and quality of naupli produced. Hormonal induction of maturation is one of alternative solution that can improve shrimp sperm quality. This study was conducted to examine the effect of pregnant mare serum gonadotropin (PMSG) and antidopamine (AD) injection on white shrimp <em>Litopenaeus vannamei</em> sperm quality. This research consisted of six treatments which were treatment without eyestalk ablation, eyestalk ablation, and premix PMSG hormone, and AD at the dose of 0.1 mL/kg, 0.25 mL/kg, 0.5 mL/kg, and 1 mL/kg. The observed parameters were sperm count and percentage of normal and abnormal sperm. The results showed that PMSG hormone and AD injection could improve sperm quality of <em>L. vannamei</em> shrimp. Hormone at the dose of 0.25 mL/kg and 0.5 mL/kg were the optimal doses to increase sperm count and the percentage of normal sperm, also to lower the percentage of abnormal sperm.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keyword: PMSG, AD, sperm quality, <em>Litopenaeus vannamei</em></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kuantitas dan kualitas sperma udang jantan sangat berperan penting dalam menentukan produktivitas udang. Terjadinya penurunan daya tetas telur udang diduga karena terjadinya penurunan kualitas sperma. Hal ini berpengaruh terhadap jumlah dan kualitas nauplius yang diproduksi. Induksi maturasi secara hormonal merupakan salah satu alternatif yang dapat meningkatkan kualitas sperma udang. Penelitian ini dilakukan untuk mengkaji pengaruh penyuntikan <em>pregnant mare serum gonadotropin</em> (PMSG) dan antidopamin (AD) terhadap kualitas sperma udang vaname <em>Litopenaeus vannamei</em>. Penelitian terdiri atas enam perlakuan, yaitu perlakuan tanpa ablasi mata, ablasi mata, dan injeksi dengan premix hormon PMSG dan AD dosis 0,1 mL/kg, 0,25 mL/kg, 0,5 mL/kg, dan 1 mL/kg. Parameter yang diamati jumlah sperma, persentase sperma normal dan abnormal. Hasil penelitian menunjukkan penyuntikan hormon PMSG dan AD dapat meningkatkan kualitas sperma udang <em>L. vannamei</em>. Hormon dosis 0,25 mL/kg dan 0,5 mL/kg merupakan dosis optimal dalam meningkatkan jumlah sperma dan persentase sperma normal, serta mengurangi persentase sperma abnormal.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: PMSG, AD, kualitas sperma, <em>Litopenaeus vannamei</em></p><p> </p>
This development aims to develop teaching materials for writing short stories with adobe flash SMP Class IX. The scope of this development is limited only to planning, material and evaluation in the learning process. The method used in this research is Research and Development (R&D), using a development research design using the Brog & Gall model. Teaching material development results obtained material expert test results 82% were declared feasible, media expert test 86% were declared feasible, small group field test 80% declared good, a large group field test 82.3% was declared good. So overall the data obtained from the development of instructional materials with adobe flash media is declared feasible, teaching materials with adobe flash media are effective in increasing motivation, focus and responsiveness while the evaluation results provide increased student ability in writing short stories.
This research aims to analyze the effectiveness of HOTS learning and self efficacy of students on economic literacy, the population of grade XI SMAN 11 Tangerang city as much as 109, sampled using Proportionate Simple Random Sampling through sloven formula so as to be a sample number of 90 students. The result of his research is that together the effectiveness of HOTS learning and student self efficacy have a significant effect on economic literacy, The effectiveness of HOTS learning has a significant effect on economic literacy, Self efficacy of students has a significant effect on economic literacy.
Madrasah Ibtidaiyah (MI) Nurul Huda must provide quality education services and be supported by information technology in improving academic and non-academic quality. This study aims to determine information technology-based management systems at MI Nurul Huda and to analyze information technology-based management systems in improving the quality of student output at MI Nurul Huda. This research uses a descriptive qualitative method. Data collection techniques used are interviews, observation, and documentation. The data analysis technique used is data reduction, data display, and conclusion drawing. The subjects in this study were the principal, the vice principal, and the teacher council. Based on the results of this study it can be concluded that the information technology management system that takes place at MI Nurul Huda is computer-based learning, E-Learning, data-based applications, E-Rapot, Fingerprint, PPDB Online, and also webinars; efforts made by madrasas to improve the quality of output at MI Nurul Huda include: organizing digital-based learning activities during the Covid-19 pandemic and carrying out face-to-face learning assisted by technology, although it is still limited to improving the quality of teaching and learning; sending talented students to take part in academic and non-academic competitions, motivating students, and providing directions to students who wish to continue to a higher level, the use of information technology can make a significant contribution in improving the quality of student output both in data management students, the learning process, a collaboration between teachers, students, and parents.
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