Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities

Rodrigues, Amélia Miranda Gomes; Guimarães, Denise O.; Konno, Tatiana Ungaretti Paleo; Tinoco, Luzineide W.; Barth, Thiago; Aguiar, Fernando Nalesso; Lopes, Norberto P.; Leal, Ivana Corrêa Ramos; Raimundo, Juliana Montani; Muzitano, Michelle Frazão

doi:10.3390/molecules22020304

Cited by 10 publications

(12 citation statements)

References 43 publications

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“…In the present study, the MVB was pre-contracted with phenylephrine and wortmannin, a PI3K inhibitor. Wortmannin strongly reduced lythrine-induced vasodilatation, suggesting that PI3K-dependent activation of eNOS is an important underlying mechanism in the vasodilatory effect of lythrine, similar to that reported for Tapirira guianensis (Rodrigues et al, 2017).…”

Section: Discussionsupporting

confidence: 84%

Evaluation of antihypertensive and vasorelaxant effects of Heimia salicifolia (family: Lythraceae)

Guzmán-Hernández¹,

Segura-Cobos²,

Amato³

et al. 2018

Afr. J. Pharm. Pharmacol.

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There are reports of the presence of vasodilatory alkaloids in Heimia salicifolia extracts. The action mechanisms of the potential antihypertensive effect of such alkaloids have not however, been elucidated. The objective of the present study was to corroborate the antihypertensive and vasorelaxant activities of the isolated alkaloids from H. salicifolia, and to characterize their vasorelaxant actions. A chloroform extract (HSCE) was obtained from H. salicifolia. The alkaloids were separated by thin layer chromatography, extracted from silica gel, and evaluated using spectroscopy. The alkaloid with the most potent vasorelaxant activity was identified as lythrine. The antihypertensive effect of the HSCE was corroborated on normotensive rats and on animals with N ω -nitro-L-arginine-methyl ester (L-NAME) induced hypertension. The action mechanisms of the HSCE and lythrine were studied on isolated and perfused rat mesenteric vascular bed (MVB) preparations. HSCE administration produced a concentration-dependent relaxation, but in preparations without vascular endothelium, the relaxant response to HSCE was significantly diminished. In MVB preparations with intact vascular endothelium, pre-contracted with phenylephrine or L-NAME, perfusion with lythrine produced concentrationdependent relaxation; a similar effect was produced by acetylcholine. Wortmannin (phosphatidylinositol 3-kinase inhibitor) and methylene blue (guanylate cyclase inhibitor) decreased the relaxant effect of lythrine. These data suggest that the vascular relaxation induced by alkaloids isolated from H. salicifolia is dependent on the activation of the nitric oxide/guanylate cyclase pathway.

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Section: Discussionsupporting

confidence: 84%

Evaluation of antihypertensive and vasorelaxant effects of Heimia salicifolia (family: Lythraceae)

Guzmán-Hernández¹,

Segura-Cobos²,

Amato³

et al. 2018

Afr. J. Pharm. Pharmacol.

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“…The main compounds from T. guianesis were glycosylated flavo- S6). From plants collected in distinct biomes, these compounds have been described already by Correia et al, 27 and Rodrigues et al, 28 showing that these compounds could be used as chemical markers of this species.…”

Section: Characterisation Of Secondary Metabolites By Hlpc-dad-ms/msmentioning

confidence: 74%

Application of the metabolomics approach to the discovery of active compounds from Brazilian trees against resistant human melanoma cells

Kato

Stavis

Boaretto

et al. 2021

Phytochemical Analysis

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Introduction:The chemical diversity of plants plays an essential role in the development of new drugs. However, new bioactive compound identification and isolation are challenging due to the complexity and time-consuming nature of the traditional process. Recently, alternative strategies have become popular, such as the statistical approach to correlate compounds with biological activities, overcoming bottlenecks in bioactive natural product research.Objective: We aimed to determine bioactive compounds against resistant human melanoma cells from leaves of Aspidosperma subincanum, Copaifera langsdorffii, Coussarea hydrangeifolia, Guarea guidonea and Tapirira guianensis, using a metabolomics approach. Material and Methods:The extracts and fractions were obtained by accelerated solvent extraction (ASE) and tested against resistant melanoma cells SK-MEL-28 and SK-MEL-103. Chemical analysis was performed by high-performance diode array detector tandem mass spectrometry (HPLC-DAD-MS/MS). Chemical and biological data were analysed through univariate and multivariate analysis. Results:The species present high chemical diversity, including indole alkaloids, glycosylated flavonoids, galloylquinic acid derivatives, cinnamic acid derivatives, and terpenes. The ASE fractionation separated the compounds according to the physicochemical properties; only C. langsdorffii and T. guianensis extracts were active.Both results from the chemical profile and the biological assay were treated using a metabolomics approach to identify the contribution of different classes of secondary metabolites in the viability of human melanoma cells. The analyses showed the metabolites from C. langsdorffii and T. guianensis, such as polyphenols and terpenes, were the main compounds correlated with the biological response. Conclusion:These findings afford alternative pathways that are trustworthy and less time-consuming to identify new bioactive compounds against multidrug-resistant human melanoma cells.

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“…In the past decade, epidemiological studies have shown that cardiovascular diseases are the main cause of death and disability worldwide [1,2]. In Africa, cardiovascular diseases (CVD) have reached nearly epidemic proportions.…”

Section: Introductionmentioning

confidence: 99%

“…The pathophysiological mechanism behind this disorder is multifactorial and include oxidative stress, inflammation, reninangiotensin system and autoimmune vascular dysfunction [5][6][7]. Hypertension is characterized by a chronic elevation of arterial blood pressure (superior or equal to 140/90 mmHg), in which abnormally increased vascular tone plays a major role in the maintenance of high blood pressure [2,8].…”

Section: Introductionmentioning

confidence: 99%

Endothelium-Independent Vasorelaxant Effects of Anthocyanins-Enriched Extract from Odontonema strictum (Nees) Kuntze (Acanthaceae) Flowers: Ca2+ Channels Involvement

Nitiéma

Koala

Belemnaba

et al. 2019

EJMP

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Aims:We aimed in this study to investigate the mechanisms of the vasorelaxation effect caused by the anthocyanins-enriched extract of Odontonema strictum flowers. Study Design: Anthocyanins-enriched extract of Odontonema strictum flowers and vasorelaxantes activities of mice aortic rings. Place and Duration of Study: The flowers of Odontonema strictum (Nees) Kuntze (Acanthaceae) were collected in January 2015 at the "Institut de Recherche en Sciences de la Santé (IRSS)" experimental station in Ouagadougou. The experiments were conducted in October -November 2018 at the department of Medicine and Traditional Pharmacopeia-Pharmacy (MEPHATRA-PH)/IRSS. Methodology: The extract was enriched in anthocyanins using Amberlite XAD-7 non-ionic resin column. The vasorelaxant activity of anthocyanins-enriched extract of O. strictum flowers (OSF) was tested using isolated organ-chamber technique with mice aorta rings. Results: OSF showed concentration-dependent relaxant effects on mice endothelium intact or denuded aortic rings pre-contracted with U46619 (10 -7 M) and KCl (80 mM). OSF induced relaxation in the mice aortic rings by stimulating smooth muscle cells. The vasorelaxant effect of OSF (10-1000 µg/mL) was similar in endothelium-intact and endothelium-denuded aortic rings. The maximum relaxant effect was 93.78 ± 4.69% and 92.30 ± 3.19% for endothelium-intact and endothelium-denuded aortic rings, respectively. Moreover, after incubation of the aorta rings with OSF (400 µg/mL) or vehicle (0.02% of DMSO) in PSS, OSF blocked the contraction through mechanism involving inhibition of CaCl 2 and U46619 effect. Conclusions:The present study provides a pharmacological evidence for the antihypertensive medicinal use of Odontonema strictum by highlighting its vasorelaxant activity.

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Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities

Cited by 10 publications

References 43 publications

Evaluation of antihypertensive and vasorelaxant effects of Heimia salicifolia (family: Lythraceae)

Evaluation of antihypertensive and vasorelaxant effects of Heimia salicifolia (family: Lythraceae)

Application of the metabolomics approach to the discovery of active compounds from Brazilian trees against resistant human melanoma cells

Endothelium-Independent Vasorelaxant Effects of Anthocyanins-Enriched Extract from Odontonema strictum (Nees) Kuntze (Acanthaceae) Flowers: Ca2+ Channels Involvement

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