Endothelium-Independent Vasorelaxant Effects of Anthocyanins-Enriched Extract from Odontonema strictum (Nees) Kuntze (Acanthaceae) Flowers: Ca2+ Channels Involvement

Abstract

Aims:We aimed in this study to investigate the mechanisms of the vasorelaxation effect caused by the anthocyanins-enriched extract of Odontonema strictum flowers. Study Design: Anthocyanins-enriched extract of Odontonema strictum flowers and vasorelaxantes activities of mice aortic rings. Place and Duration of Study: The flowers of Odontonema strictum (Nees) Kuntze (Acanthaceae) were collected in January 2015 at the "Institut de Recherche en Sciences de la Santé (IRSS)" experimental station in Ouagadougou. The experiments were conducted in October -November 2018 at the department of Medicine and Traditional Pharmacopeia-Pharmacy (MEPHATRA-PH)/IRSS. Methodology: The extract was enriched in anthocyanins using Amberlite XAD-7 non-ionic resin column. The vasorelaxant activity of anthocyanins-enriched extract of O. strictum flowers (OSF) was tested using isolated organ-chamber technique with mice aorta rings. Results: OSF showed concentration-dependent relaxant effects on mice endothelium intact or denuded aortic rings pre-contracted with U46619 (10 -7 M) and KCl (80 mM). OSF induced relaxation in the mice aortic rings by stimulating smooth muscle cells. The vasorelaxant effect of OSF (10-1000 µg/mL) was similar in endothelium-intact and endothelium-denuded aortic rings. The maximum relaxant effect was 93.78 ± 4.69% and 92.30 ± 3.19% for endothelium-intact and endothelium-denuded aortic rings, respectively. Moreover, after incubation of the aorta rings with OSF (400 µg/mL) or vehicle (0.02% of DMSO) in PSS, OSF blocked the contraction through mechanism involving inhibition of CaCl 2 and U46619 effect. Conclusions:The present study provides a pharmacological evidence for the antihypertensive medicinal use of Odontonema strictum by highlighting its vasorelaxant activity.

Anthocyanins: Promising Natural Products with Diverse Pharmacological Activities

“…High Blood Pressure Ribes nigrum C3G, C3R, D3G, D3R ↑eNOS mRNA levels ↑NO synthesis [11] Odontonema strictum ↓CaCl2 and U46619 effect [12] Diabetic Heart Disease Vaccinium vitis-idaea C3G, C-3-Ara, C-3-Gal ↑Antioxidant [13] Oryza sativa C3G ↓TLR4/NFκB ↓active hypertrophy ↓Fibrosis associated molecular marker [14] Oryza sativa C3G ↑Survival signals ↓Apoptosis and the associated proapoptotic proteins [15] Table 1. Cont.…”

“…Black currant extract (BCE) contains high anthocyanins concentrations and increases NO synthesis via endothelial nitric oxide synthase, which are critical regulators of cardiovascular disease [11]. Anthocyanin-enriched extracts of Odontonema strictum flowers can block the contraction of aortic rings because anthocyanin (400 µg/mL) inhibits the effects of CaCl 2 and a thromboxane A 2 analog agonist (U46619) in physiological salt solution [12]. Blood pressure was significantly reduced after consumption of 300 mL of anthocyanin-rich cherry in older adults [73].…”

Phytochemical Profile, Acute Toxicity, Antioxidant and Anti-inflammatory Activity of Hydroethanolic Extracts from Odontonema strictum (Nees) Kuntze (Acanthaceae) Root and Stem

“…Odontonema strictum root and stem and Trolox free radical scavenging activity were performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) [21]. Briefly, after 30 min of incubation in the dark at room temperature, the absorbance of 10 μL of samples and Trolox added to 200 μL of DPPH (0.04 mg/mL) was measured at 490 nm using a Bio-Rad spectrophotometer (model 680, Japan).…”

Phytochemical Screening by High-Performance Thin-Layer Chromatography, Antioxidant Activities and Acute Toxicity of Trunk Barks Extracts of <i>Lannea velutina</i> A. Rich

“…Due to their potential pharmacological effects and commercial viability, the medicinal properties of plants have been investigated worldwide [25] in light of contemporary scientific breakthroughs. Numerous aromatic and medicinal plants include antioxidant-active chemical compounds (flavonoids, tannins, sterols, triterpenes) [20] [26]. Observations have shown that phenolic compounds inhibit amylase and glucosidase associated with lipid peroxidation, type 2 diabetes, and blood pressure regulation [27].…”