Phytochemical screening and characterization of bioactive compounds from Juniperus squamata root extract

Rana, Anita; Negi, Pushpa Bhakuni; Sahoo, Nanda Gopal

doi:10.1016/j.matpr.2021.07.305

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…These secondary metabolites played an insignificant role in the growth of the plant. However, they were essential for various defense mechanisms against the harmful effects of UV radiation, herbivore, and microbial attack [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo anthelmintic and chemical studies of Cyperus rotundus L. extracts

El-Wakil

Shaker

Aboushousha

et al. 2023

BMC Complement Med Ther

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Background Trichinellosis, a zoonosis caused by the genus Trichinella, is a widespread foodborne disease. Albendazole, one of the benzimidazole derivatives, is used for treating human trichinellosis, but with limited efficacy in killing the encysted larvae and numerous adverse effects. Cyperus rotundus L. is a herbal plant with a wide range of medicinal uses, including antiparasitic, and is frequently used in traditional medicine to treat various illnesses. Methods LC-ESI-MS was used to identify the active phytoconstituents in the methanol extract (MeOH ext.) of the aerial parts of C. rotundus and its derivate fractions ethyl acetate (EtOAc fr.), petroleum ether (pet-ether fr.), and normal butanol (n-BuOH fr.). The in vivo therapeutic effects of C. rotundus fractions of the extracts were evaluated using the fraction that showed the most promising effect after detecting their in vitro anti-Trichinella spiralis potential. Results C. rotundus extracts are rich in different phytochemicals, and the LC-ESI-MS of the 90% methanol extract identified 26 phenolic compounds classified as phenolic acids, flavonoids, and organic acids. The in vitro studies showed that C. rotundus extracts had a lethal effect on T. spiralis adults, and the LC50 were 156.12 µg/ml, 294.67 µg/ml, 82.09 µg/ml, and 73.16 µg/ml in 90% MeOH ext., EtOAc fr., pet-ether fr. and n-BuOH fr., respectively. The n-BuOH fr. was shown to have the most promising effects in the in vitro studies, which was confirmed by scanning electron microscopy. The in vivo effects of n-BuOH fr. alone and in combination with albendazole using a mouse model were evaluated by counting adults in the small intestine and larvae in the muscles, in addition to the histopathological changes in the small intestine and the muscles. In the treated groups, there was a significant decrease in the number of adults and larvae compared to the control group. Histopathologically, treated groups showed a remarkable improvement in the small intestine and muscle changes. Remarkably, maximal therapeutic effects were detected in the combination therapy compared to each monotherapy. Conclusion Accordingly, C. rotundus extracts may have anti-T. spiralis potential, particularly when combined with albendazole, and they may be used as synergistic to anti-T. spiralis medication therapy.

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Section: Discussionmentioning

confidence: 99%

In vitro and in vivo anthelmintic and chemical studies of Cyperus rotundus L. extracts

El-Wakil

Shaker

Aboushousha

et al. 2023

BMC Complement Med Ther

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“…Phytochemical analysis was done to find the presence of alkaloids, terpenoids, steroids, phenols, flavonoids, tannins, glycosides, and saponins [47]. The role of these constituents in Table 7, for the improvement of strength and durability properties, was vital.…”

Section: Phytochemical Analysismentioning

confidence: 99%

Properties of Air Lime Mortar with Bio-Additives

Manoharan

Umarani

2022

Sustainability

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Lime mortar has been a primary binding material in ancient mortar, and is one of the main reasons behind solid and stable constructions that remain stable even after thousands of years. The benefits of lime are innumerable: it is minimally processed and used with a lesser carbon footprint and embodied energy and, most crucially, it is a carbon absorbent. This research experiments with the strength properties (compression) of lime at 28, 56, and 100 days of air curing. The investigation studies the durability using water absorption, UPV test, and carbonation parameters after 100 days of exposure to air. The tested materials are subjected to SEM analysis to find the morphology of the reaction that takes place and the products that are formed. We also performed a comparative study of two different fermented additives by the duration of fermentation (1 day and 10 days) and two different doses of additives (Jaggery and Kadukkai) with air lime. The bio-additives were experimented with using gas chromatography and mass spectroscopy for the formation of new enriching compounds, which improved the qualities of traditional lime mortar. The formation of fat and protein in the additives was found using IS 7219-1973 (a method for the determination of protein in foods and feeds). Using the AOAC method, the presence of fat confirms the improvement in strength and durability properties. The phytochemical analysis details the alkaloids, terpenoids, steroids, phenols, flavonoids, tannins, glycosides, and saponins. Quantification of phenols and flavonoids adds to the beneficial aspects of the fermented additives. The experimental results indicate that using naturally fermented organic materials in the lime has made the structures stronger with the stable build of calcite and vaterite components. The self-healing capacity of lime mortar makes it time resistant.

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“…After a period of settling for 5 minutes, the supernatant was filtered through a white cloth and then three times through cotton wool. The filtrate was dried in a Med Center Venticell oven at 55 °C for 48 hours to obtain the dry extract [10].…”

Section: Preparation and Extraction Of Plant Materialsmentioning

confidence: 99%

Phytochemical analysis, investigation of antioxidant and anti-inflammatory activities of ethanolic and aqueous extracts of roots of Combretum glutinosum Perr. ex DC from Cote d'Ivoire

2022

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The phytochemical, antioxidant, and anti-inflammatory potential of root (ethanol and aqueous) extracts of Combretum glutinosum was investigated in this study. Their antioxidant activity was determined using an in vitro DPPH radical scavenging activity assay. The ethanol extract had the lowest IC50 (0.055 mg/mL), which is comparable to vitamin C. Phytochemical screening of extracts revealed the presence of sterols and polyterpenes, polyphenols, alkaloids, flavonoids, catechin tannins, gallic tannins, saponosides, terpenoids, mucilages, anthocyanins, volatile oils, and cardiac glycosides. The extracts significantly inhibit the development of paw edema induced by carrageenan. Anti-inflammatory studies showed that the inflammation inhibition potential of 200 mg/kg body weight of all extracts was significantly lower than the standard diclofenac (20 mg/kg) in the first hours. At the third hour, the inflammation inhibition potential of ethanolic and aqueous extracts was significantly higher than that of the standard. This study revealed that Combretum glutinosum extracts have anti-inflammatory effects and can act as an effective antioxidant.

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Phytochemical screening and characterization of bioactive compounds from Juniperus squamata root extract

Cited by 4 publications

References 5 publications

In vitro and in vivo anthelmintic and chemical studies of Cyperus rotundus L. extracts

In vitro and in vivo anthelmintic and chemical studies of Cyperus rotundus L. extracts

Properties of Air Lime Mortar with Bio-Additives

Phytochemical analysis, investigation of antioxidant and anti-inflammatory activities of ethanolic and aqueous extracts of roots of Combretum glutinosum Perr. ex DC from Cote d'Ivoire

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