Phytochemical analysis, investigation of antioxidant and anti-inflammatory activities of ethanolic and aqueous extracts of roots of Combretum glutinosum Perr. ex DC from Cote d'Ivoire

Abstract

The phytochemical, antioxidant, and anti-inflammatory potential of root (ethanol and aqueous) extracts of Combretum glutinosum was investigated in this study. Their antioxidant activity was determined using an in vitro DPPH radical scavenging activity assay. The ethanol extract had the lowest IC50 (0.055 mg/mL), which is comparable to vitamin C. Phytochemical screening of extracts revealed the presence of sterols and polyterpenes, polyphenols, alkaloids, flavonoids, catechin tannins, gallic tannins, saponosides, terpenoids, mucilages, anthocyanins, volatile oils, and cardiac glycosides. The extracts significantly inhibit the development of paw edema induced by carrageenan. Anti-inflammatory studies showed that the inflammation inhibition potential of 200 mg/kg body weight of all extracts was significantly lower than the standard diclofenac (20 mg/kg) in the first hours. At the third hour, the inflammation inhibition potential of ethanolic and aqueous extracts was significantly higher than that of the standard. This study revealed that Combretum glutinosum extracts have anti-inflammatory effects and can act as an effective antioxidant.

In Vitro Filaricidal Properties of Aqueous Extracts of Combretum nigricans (Combretaceae) on Onchocerca ochengi (Onchocercidae)

“…First of all, the bark aqueous extract had the highest extraction yield (10%) compared to leaves and root extracts ( Table 1 ), suggesting it concentrates more hydrosoluble secondary metabolites. This finding differs from previous studies that found a yield of 15.30% with aqueous extracts of Combretum glutinosum roots [ 43 ] and 5.70% with Combretum micranthum leaves [ 44 ]. Although all these plants belong to the same family, this difference could be explained either by the time and place of the harvest of each plant, the secondary metabolites present in each species, and even the climatic and environmental conditions or the volume of the solvent used and the mass of the vegetable powder.…”

Contribution to the phytochemical study and evaluation of the antibacterial activity of extracts from the leaves of Ageratum conyzoides (Asteraceae) from Côte d'Ivoire

“…Terpenoids are widely used for their aromatic qualities. They also play a role in traditional herbal remedies and are the subject of research into their antibacterial and antineoplastic effects [41]. However, the terpenes and sterols derived from terpenoids are analgesic and anti-inflammatory [9].…”

Revue des usages traditionnels, composition chimique et propriétés pharmacologiques de <i>Combretum glutinosum</i> (Combretaceae)

“…Elles ont plus porté sur les feuilles. Le criblage phytochimique des extraits de feuilles, de racines, de tiges, d'écorces et de galles de C. glutinosum a révélé la présence de stérols, polyterpènes, de polyphénols, de saponosides, de flavonoïdes, de tanins catéchiques, de quinones et ainsi que d'alcaloïdes, de coumarines, d'anthraquinones, d'hétérosides cardiotoniques et de cyanogènes (Sore et al, 2012;Yahaya et al, 2012;Niass et al, 2017;Sall et al, 2017;Sène et al, 2020;Tahiri and Kouame, 2022;Sweilam et al, 2023;Toklo et al, 2023). Sore et al (2012) ont déterminé la teneur en extraits phénoliques totaux des feuilles de C. glutinosum ; elle était de 68,27 ± 0,90 µg EAG/100 mg (microgramme équivalent d'acide gallique par milligramme) (Sore et al, 2012).…”

“…Récemment, Tahiri et al (2022) ont évalué les effets analgésiques des extraits aqueux et éthanoliques des racines de C. glutinosum en utilisant la méthode de contorsion induite par l'acide acétique sur des souris. L'étude a montré que ces extraits ont des effets analgésiques (Tahiri et al, 2022).…”