Phytochemical and in vitro anti-bacterial properties of Hibiscus sabdariffa L (Roselle) juice

Abstract

The anti-bacterial properties of aqueous and ethanol extracts of the calyces of Hibiscus sabdariffa on five bacteria genera, namely, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Shigella dysenteriae and Streptococcus mutans were evaluated using agar well and disc diffusion methods. Six bioactive compounds were identified from phytochemical analysis of the juice extract of H. sabdariffa. Analysis revealed the presence of the following in the water and ethanol extracts: saponins (1.46%), alkaloids (0.09%), tannins (0.19%), total phenols (0.07%), flavonoids (2.41%) and glycosides (0.13%). The results revealed that both water and ethanol extracts had significant anti-bacterial effects (P ≤ 0.05) against the tested pathogenic bacteria genera with the ethanol extract of Roselle having higher antibacterial effects against all the pathogens. S. aureus and E. coli were the most sensitive to Roselle juice extract (47.9 and 45. 8 mm zones of inhibition, respectively). The minimum inhibitory concentration (MIC) for the ethanol extract of H. sabdariffa was between 0.26 and 1.03 mg/ml. The results observed in this study contribute to scientific baseline data on the anti-bacterial activities of H. sabdariffa juice extract.

In vivo anti-staphylococcal activity of roselle (Hibiscus sabdariffa L.) calyx extract in Drosophila model of infection

“…Infusion prepared from roselle calyces has been generally used by populations from India and countries of Africa as a traditional remedy to treat various illnesses, including infectious diseases (30) and its extract has been shown to yield excellent in vitro antibacterial activities against several bacteria, including S. aureus (12,13). However, until now, limited information was available regarding the in vivo anti-staphylococcal activity of this particular preparation.…”

“…One of promising sources for such efforts is roselle (Hibiscus sabdariffa L.). The ethanolic extract of roselle's calyx has been shown to yield excellent in vitro antibacterial effect against several Gram-positive pathogenic bacteria (9)(10)(11), including S. aureus and MRSA (12,13). However, despite such promising antibacterial effect, no in vivo experiment has been carried out to further clarify the antistaphylococcal activity of roselle calyx ethanolic extract.…”

The Effect Of Roselle Extract (Hibiscus sabdariffa Linn.) On Blood Glucose Level And Total Antioxidant Level On Diabetic Rat Induced By Streptozotocin

“…Vitamin C plays a role as scavenger, and has an ability to donate electrons to ROS to make it stable [8]. According [46] states that phytochemical contents in roselle extract in water are flavonoids (1.08%), saponins (1.13%), alkaloids (12.09%), tannins (12.07%), total phenols (0.05%), and glycosides (0.05%). Roselle is able to increase glutathione peroxidase and catalase in liver of treated rats compared with that in control mice [20].…”

Inhibitory Effect of Roselle Aqueous Extracts-HPMC 6000 Gel on the Growth of Staphylococcus aureus ATCC 25923

“…The saponins and flavonoids are the largest content. 2,3 These compounds indicate synergistic effects. Complexity compounds in the extracts have provided good antibacterial activity.…”

“…5,6,7 In term of their antimicrobial activities, the Roselle aqueous extract was used at concentration above its minimum inhibitory concentration (MIC, 3%). The low pH values of Roselle aqueous extract (2.42 ± 0.01) lead to consider the HPMC 6000 was chosen as a gelling agent at concentrations of 2 %, 3…”