Enterococcus faecium IS-27526 is a novel probiotic isolated from dadih, an Indonesian traditional fermented buffalo milk. A 90 days randomized double-blind placebo-controlled study of pre-post trial was conducted in pre-school children with two groups, placebo and probiotic group. Ultra High Temperature low fat milk was used as a carrier in each group. The aims of this study were to investigate the effect of E. faecium IS-27526 in milk on humoral immune response and on bodyweight of pre-school children. Total serum IgA and total salivary sIgA were measured by sandwich ELISA. The bodyweight of young children was measured. The results showed that total serum IgA did not significantly increase in the probiotic group compared with the placebo group. Total salivary sIgA level and the bodyweight significantly increased (p < 0.05) in probiotic groups compared to the placebo. Changes of total salivary sIgA level were significantly higher in underweight children supplemented with probiotic. Weight gain was observed significantly in children with normal bodyweight supplemented with probiotic. Neither mortality nor weight loss was recorded throughout the study. Taken together, novel probiotic E. faecium IS-27526 has significant positive effects on humoral immune response, salivary sIgA, in underweight pre-school children, and on weight gain of pre-school children.
ABSTRAKRumput laut jenis Ulva lactuca dan Eucheuma cottonii merupakan rumput laut yang dapat dijumpai di perairan Indonesia, akan tetapi pemanfaatannya sebagai produk nori belum dilakukan. Penelitian ini bertujuan untuk memproduksi nori dari rumput laut U. lactuca dan E. cottonii dan menguji karakteristik mutu fisik, kimia dan sensori. Dari hasil penelitian didapatkan bahwa campuran rumput laut U. lactuta dan E. cottonii dapat menghasilkan produk nori yang berkualitas. Hasil uj i organoleptik menunj ukkan produk nori yang dipanggang lebih dapat diterima dibandingkan nori tidak dipanggang. Karakteristik kimia dan fisik produk berbeda nyata pada taraf 5%. Hasil analisis karakteristik kimia menunjukkan bahwa produk nori rumput laut U. lactuca dan E. cottonii mempunyai kandungan protein yang lebih rendah (18.84%), kandungan karbohidrat yang lebih tinggi (62.31%), kandungan serat pangan yang lebih tinggi (36.76%) dan kapasitas antioksidan yang lebih rendah (43.01%) dibandingkan nori komersial (karbohidrat 41.8%, protein 40%, serat 21.3%, kapasitas antioksidan 51%). Rumput laut di Indonesia saat ini digunakan sebagai penghasil agar, karaginan, dan alginat yang merupakan bahan baku penting dalam industri pangan maupun non pangan. Rumput laut Eucheuma cottonii yang banyak dibudidayakan di Indonesia dimanfaatkan untuk industri penghasil karaginan dan untuk produk pangan lainnya seperti untuk campuran es, dodol dan lain-lain. Rumput laut Ulva lactuca merupakan rumput laut yang tumbuh secara alami di perairan Indonesia, di mana pemanfaatannya masih sangat terbatas. KATA KUNCI : nori, rumput Laut, Ulva lactuca, Eucheuma cottonii ABSTRACT
Rumput laut merupakan sumber daya hayati yang sangat berlimpah di perairan Indonesia. Namun demikian pemanfaatannya untuk pengolahan produk pangan sangat terbatas, terutama untuk produk pangan fungsional. Rumput laut berpotensi dikembangkan sebagai produk pangan fungsional karena mengandung zat gizi dan komponen bioaktif yang berkhasiat untuk kesehatan. Rumput laut mengandung sejumlah komponen bioaktif seperti senyawa fenolik, pigmen alami, polisakarida sulfat, serat dan komponen bioaktif lainnya yang telah diteliti berkhasiat untuk kesehatan. Untuk dapat dikembangkan sebagai produk pangan fungsional, rumput laut yang digunakan harus bebas dari cemaran logam berat dan bahan pencemar lainnya, harus mengandung komponen bioaktif dan zat gizi yang tinggi sehingga harus ada penerapan standar penanaman dan penanganan pasca panen yang baik di tingkat petani rumput laut. Selain itu Proses pengolahan pangan yang diterapkan tidak merusak komponen bioaktif yang terkandung dalam rumput laut. Optimalisasi pengolahan rumput laut sebagai produk pangan fungsional merupakan alternative pemanfaatan potensi rumput laut Indonesia yang dapat meningkatkan nilai ekonomi rumput laut dan yang lebih penting dapat menyediaakan akses pangan sehat bagi masyarakat luas.Seaweed is a living resource that is abundantly available in Indonesian water. However, its utilization in food processing is very limited, especially as functional food products. Seaweed has the potential to be developed as functional food products because it has nutrient and bioactive components that are beneficial for health. Seaweed has a number of bioactive components such as phenolic compound, natural pigment, polysaccharide sulphate, fiber and other bioactive components that has been studied to be advantageous for health. For a seaweed to be developed into functional food product, it must be free from heavy metal and other pollutant contamination, and must contain bioactive components and high nutrients, thus, a good cultivation and postharvest handling standard have to be applied in seaweed farmer level. Moreover, the food processing applied should not damage the bioactive component within the seaweed. Optimization of seaweed processing into functional food product is an alternative for seaweed potential utilization in Indonesia, which could improve the economic value of the seaweed, and more importantly it could provide access for healthy food for community.
All food proteins have a potential to be allergic for some people. Nuts and legumes are some of the main food allergens, which are consumed by large numbers of people. Several researches had been reported about the allergenicity of peanut, soybean, tree nuts, but not of the bambara groundnut (Vigna subterranea), one of the legumes consumed largely by the population in Indonesia. The reported study aimed to isolate bambara groundnut protein and to analyze its allergic reactivity. The protein isolate reactivity was tested by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), enzyme-linked immunosorbent assay (ELISA) and immunoblotting, involving six peanut and soybean allergic human sera. ELISA analysis showed that the bambara groundnut protein had allergenic proteins. Furthermore, immunoblotting analysis revealed that serum IgE from the donors could bind to individual allergens to various degrees, demonstrating the specificity of IgE reactivity. In conclusion, bambara groundnut contained allergenic proteins, and the research demonstrated a cross-reactivity to peanut and soybean.
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