Physiological and isozyme alterations in lettuce seeds under different conditions and storage periods

Catão, Hugo César Rodrigues Moreira; Gomes, Luíz Antônio Augusto; Guimarães, Renato Mendes; Fonseca, Pedro Henrique Faria; Caixeta, Franciele; Marodin, Josué Clock

doi:10.1590/2317-1545v38n4163863

Cited by 12 publications

(4 citation statements)

References 23 publications

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“…The 26 F 2:3 progenies were also collected individually. The purity of the F 1 seeds was assessed by sowing the parents and the F 1 generation in a polypropylene tray, in which the occurrence of contamination was determined by differences in leaf edge: frilled and curled edges for cultivar Verônica (Silva et al, 2008), smooth edges for cultivar Everglades (Catão et al, 2016), and intermediate edges for F 1 (Silva et al, 2005). The seeds of the F 2:3 progeny were used to detect the proportion of segregation observed in F 2 , which allows inferring about the number of genes involved in the trait (Ramalho et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

Genetic control of thermoinhibition tolerance in lettuce seeds

Oliveira

Cavasin

Silva

et al. 2021

Pesq. agropec. bras.

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The objective of this work was to assess the genetic control of tolerance to thermoinhibition in lettuce (Lactuca sativa) seeds. Seeds of the F1, F2, and F2:3 generations derived from the cross between cultivars Everglades (tolerant to thermoinhibition) and Verônica (sensitive to thermoinhibition) were used, besides seeds from the parents. Seed germination tests were conducted in a completely randomized design, with four replicates of each parent, eight of F1, and four of each of the 26 F2:3 progenies. The mean of the F2 population was considered as the mean of the sample of the 26 progenies. The genetic control of thermoinhibition tolerance in lettuce seeds is attributed to one or a few genes. The additive effects are more expressive than the nonadditive ones, and narrow-sense heritability is relatively high, allowing the prediction of success in selection.

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Section: Methodsmentioning

confidence: 99%

Genetic control of thermoinhibition tolerance in lettuce seeds

Oliveira

Cavasin

Silva

et al. 2021

Pesq. agropec. bras.

Self Cite

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“…. This greater activity may be related to the high rate of seed respiration and, consequently, greater deterioration (Catão et al, 2016). The enzyme MDH performs an important function in the Krebs cycle, catalyzing the conversion of malate to oxaloacetate, producing NADH, which is a fundamental product in production of ATP and of intermediary compounds essential to cell operation (Williams and O'Neill, 2018).…”

Section: Analyses Of Cryopreserved Seedsmentioning

confidence: 99%

Cryopreservation of Coffea canephora Pierre ex A. Froehner seeds: importance of drying rate and moisture content

et al. 2019

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Seeds of the Coffea canephora species are considered more recalcitrant than those of the Coffea arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because they are sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics for long periods. However, preliminary studies are necessary to determine ideal storage conditions. The aim of this study was to investigate the ideal physical and physiological conditions for cryopreservation of Coffea canephora seeds to reduce seed mortality caused by the formation of intracellular ice crystals and to avoid cell damage caused by excessive desiccation. Seeds were subjected to rapid drying in silica gel and slow drying in saturated NaCl solution to moisture contents of 0.20, 0.25, and 0.28 g.g-¹ (dry basis), followed by direct immersion in liquid nitrogen for rapid freezing. Physiological and biochemical analyses were performed to evaluate seed quality before and after cryopreservation. Rapid drying of Coffea canephora seeds to values near 0.20 g.g-1 (db) does not cause reduction in physiological quality. Moisture content of 0.25 g.g-¹ brings about greater survival of Coffea canephora seeds after cryopreservation. Drying rate affects the physiological quality of cryopreserved Coffea canephora seeds, and rapid drying in silica gel is more favorable than slow drying in a saturated NaCl solution. The activity of the enzymes catalase, esterase, glutamic oxaloacetic transaminase, and polyphenol oxidase are indicators of seed quality for Coffea canephora seeds subjected to cryopreservation.

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“…The correct storage can favor the breakdown of thermodormency naturally during the period. However, there must be controlled by environmental factors, especially temperature, when elevated it can reduce the germination rate (Catão et al, 2016. Once in Brazil the lettuce cultivation is done majority on southeast region, and the temperatures are often found at or higher than 30 °C the thermodorment seeds cannot germinate, but lettuce genotypes that can be resistant to thermoinhibition can germinate even under higher temperature, expanding the regions where the crop can be cultivated and, consequently, reducing the seasonality of lettuce supply in tropical regions. In this sense, there is a need to grow lettuce cultivars that have a higher temperature limit for seed germination (Bufalo et al, 2012;Catão et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Enviromental temperature and age of seeds in tolerance to thermoinhibition in lettuce genotypes1

et al. 2023

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Lettuce seeds usually show germination problems that can be related to dormancy and or thermoinhibition, as well as to the genotype constitution. The knowledge of the temperature at which the thermoinhibition process begins, as well as the influence of the age of the seed on its germination, is extremely important to establish more suitable parameters for the selection of higher genotypes in breeding programs. The objective of this work was to evaluate the seed germination of three lettuce cultivars according to the enviromental temperature and the age of the seeds. The cultivars Everglades (tolerant to thermoinhibition), Luisa (medium tolerant) and Verônica (sensitive) were used. Seeds of each cultivar were evaluated by the standard germination test, first count test, germination speed index and germination test of remaining seeds. The analyses were performed at four environmental temperatures and in seven storage periods after harvest. None of the cultivars showed primary dormancy. The tolerance to thermoinhibition showed by cultivars Everglades and Luisa and the sensitivity of cultivar Verônica were confirmed. The most suitable temperature for differentiating tolerant and sensitive thermoinhibition genotypes occurred from 30.5 ºC and 260 days after harvest.

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Physiological and isozyme alterations in lettuce seeds under different conditions and storage periods

Cited by 12 publications

References 23 publications

Genetic control of thermoinhibition tolerance in lettuce seeds

Genetic control of thermoinhibition tolerance in lettuce seeds

Cryopreservation of Coffea canephora Pierre ex A. Froehner seeds: importance of drying rate and moisture content

Enviromental temperature and age of seeds in tolerance to thermoinhibition in lettuce genotypes1

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