ABSTRACT:The pathogenic fungi may be associated with the seeds during development, harvest and storage, and it can be disseminated through them. This work evaluated the seeds performance of three hybrid corn cultivars, BM 840 PRO, BM 810 and BM 207, submitted to different inoculum potentials of Fusarium verticillioides. Seeds were uniformly distributed over the medium fungal colony developed on PDA (potato dextrose agar) containing mannitol in water potential -2.0 MPa. Seeds remained in this way for different periods of contamination (0, 24, 48, 72 and 96 hours). Before and after seven months of seed storage the evaluations were performed. Seeds treated with fungicide Derosal Plus® (carbendazim + thiram, 250 mL p.c. 100 kg -1 of seeds) were used as control. The increased of inoculum potential of F. verticillioides fungus on corn seeds caused reduction in physiological quality in the potentials 72 and 96 hours of exposure before and after storage. The highest Fusarium verticillioides incidence is observed in non-stored seeds. Fusarium verticillioides incidence on corn seeds accentuates the deterioration in storage.
-The accelerated aging test is recognized as an efficient method for evaluating the vigor of seed lots and for estimating their storage potential. Thus, this work aimed to evaluate the association between artificial aging and natural storage of coffee seeds, through the correlation factor analysis. Seeds of four cultivars of Coffea arabica L. (Catuaí Amarelo, Arara, Catiguá, and Mundo Novo) and one of Coffea canephora Pierre (Apoatã) were used. Part of the newly-harvested seeds were aged in a growth chamber under controlled temperature and relative humidity conditions (42 ºC and 100% RH) for periods of 0, 4, 6, 8, and 10 days. The other part of the seeds was stored in tri-wall paper packaging for a period of 2, 4, and 6 months in a non-climate-controlled environment. Artificial aging allows predictions on the storage potential of coffee seeds, although the artificial aging periods depend on the cultivars.
Seeds of the Coffea canephora species are considered more recalcitrant than those of the Coffea arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because they are sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics for long periods. However, preliminary studies are necessary to determine ideal storage conditions. The aim of this study was to investigate the ideal physical and physiological conditions for cryopreservation of Coffea canephora seeds to reduce seed mortality caused by the formation of intracellular ice crystals and to avoid cell damage caused by excessive desiccation. Seeds were subjected to rapid drying in silica gel and slow drying in saturated NaCl solution to moisture contents of 0.20, 0.25, and 0.28 g.g-¹ (dry basis), followed by direct immersion in liquid nitrogen for rapid freezing. Physiological and biochemical analyses were performed to evaluate seed quality before and after cryopreservation. Rapid drying of Coffea canephora seeds to values near 0.20 g.g-1 (db) does not cause reduction in physiological quality. Moisture content of 0.25 g.g-¹ brings about greater survival of Coffea canephora seeds after cryopreservation. Drying rate affects the physiological quality of cryopreserved Coffea canephora seeds, and rapid drying in silica gel is more favorable than slow drying in a saturated NaCl solution. The activity of the enzymes catalase, esterase, glutamic oxaloacetic transaminase, and polyphenol oxidase are indicators of seed quality for Coffea canephora seeds subjected to cryopreservation.
Enzymes play a fundamental role in degradation of molecules during seed germination, development, and deterioration. Endo-β-mannanase is one of the main enzymes responsible for hydrolysis of mannans in the endosperm during germination of coffee seeds through its action in hydrolytic degradation of cell walls and in weakening the structures of the endosperm that surround the embryo, allowing radicle emergence. The aim of this study was to determine the activity of the endo-β-mannanase enzyme in the structures of coffee seeds for the purpose of assessing the relationship between this activity and the physiological quality of the seeds under different processing and drying methods. Coffea arabica L. fruit in the cherry maturity stage was subjected to three different types of processing: natural (seeds maintained in the fruit itself), fully washed (fruit pulped mechanically and the seeds demucilaged by fermentation in water), and semi-washed or demucilaged (both fruit pulp and mucilage removed mechanically); and two methods of drying: slow drying (suspended screen) in the shade, and rapid drying in mechanical dryer at 35°C to a moisture content of 11±1%. After processing and drying, the physiological quality of the seeds was evaluated through the germination test, and endo-β-mannanase enzyme activity was quantified. Coffee seeds submitted to natural processing have lower physiological performance, as well as greater deterioration and greater activity of the endo-ß-mannanase enzyme. Removal of mucilage during fully washed and semi-washed processing of coffee seeds reduces the activity of the endo-ß-mannanase enzyme and lowers deterioration, especially after faster drying. The enzyme endo-ß-mannanase is efficient in studying of the effects of processing and drying on coffee seeds, and can be evaluated in whole seeds, endosperms or embryos.
In the Brazilian National System of Seeds and Seedlings, coffee seeds are evaluated by the germination or tetrazolium test. However, differences have been observed between the results of these tests in various studies, especially when the seeds have a lower level of quality. Given this situation, the aim of this study was to evaluate the correlation between historical data of results of the germination test and of the tetrazolium test in samples of coffee seeds using Generalized Additive Models for Location, Scale and Shape (GAMLSS). Historical data of results of the germination test and of the tetrazolium test of coffee seeds originating from different cultivars and different crop seasons were used. The zero-or-one inflated beta GAMLSS is suitable for fitting data from the germination test and from the tetrazolium test. The estimate of viability by the tetrazolium test varies according to the germination percentage class. There are greater GAMLSS correlations between the percentages of normal seedlings and of viability in the tetrazolium test for germination values above 70%, and low correlations below this value, showing that evaluation of coffee seeds based only on the tetrazolium test may not correspond to actual physiological performance.
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