Pengaruh Penyalutan Terhadap Karakteristik Fisika Kimia dan Stabilitas Tablet Fraksi Etil Asetat Daun Gambir sebagai Agen Antidislipidemia

Yunarto, Nanang; Sulistyowati, Indah; Kurniatri, Arifayu Addiena; Aini, Nurul

doi:10.22435/mpk.v27i2.5873.

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the process, ethyl acetate is added through the top of the column. The fraction results were concentrated with a rotary evaporator at a temperature of 40°C, then dried using a vacuum oven at 40°C to a fixed weight (Yunarto et al, 2017;Kurniatri et al, 2019). The obtained fraction was calculated to yield fraction, organoleptic testing, moisture content, and ash content.…”

Section: Fractionationmentioning

confidence: 99%

VALIDATION OF SPECTROPHOTOMETRY METHOD FOR DETERMINATION OF (+)-CATECHIN IN ETHYL ACETATE FRACTION OF GAMBIR EXTRACT (Uncaria gambir Roxb.)

Yunarto

Reswandaru

Sulistyowati³

et al. 2021

J. Tumbuhan Obat Indonesia

View full text Add to dashboard Cite

ABSTRACT Gambir leaves (Uncaria gambir Roxb.) is a plant that has been widely used by people for traditional medicine. The main compound of gambir extract is a (+)-catechin that has been proven as anti-dental plaque, antioxidant, antibacterial, and antihyperlipidemic. This study aims to validate a method for the quantitative determination of catechin in ethyl acetate fraction of gambir extract based on spectrophotometry. The validation was conducted by measuring the linearity, accuracy, and ruggedness of the method that fulfills the requirements along with the limit of detection (LoD) and limit of quantification (LoQ) determination. Determining the precision (based on %RSD and CV), 100 ppm of catechin were made to 7 replicates while accuracy was evaluated by calculating the recovery. The ruggedness of the method is determined by analyzing it on a different day. The linearity was determined by assessing the r-value on the relation between the absorbance curve and standard series concentration. The LoD and LoQ could be calculated based on the standard curve equation. The results showed the method fulfilled the linearity requirement with R 0.9996; had precision in 1.23% RSD; had accuracy in the range of 100.80% to 101.64%; the method ruggedness was not significantly different; had 3.85 ppm of LoD and 12.84 ppm of LoQ. The validation method showed a valid result, and the method can be used for routine analysis on the measurement of catechin in ethyl acetate fraction of gambir extract. Keywords: Catechin, Uncaria gambir, Method Validation, Spectrophotometry ABSTRAK Daun gambir (Uncaria gambir Roxb.) merupakan tanaman yang telah banyak dimanfaatkan masyarakat dalam pengobatan tradisional. Senyawa utama ekstrak gambir adalah (+)-katekin yang telah terbukti sebagai anti plak gigi, antioksidan, antibakteri, dan antihiperlipidemia. Penelitian ini bertujuan untuk memvalidasi suatu metode penentuan kuantitatif katekin dalam fraksi etil asetat ekstrak gambir secara spektrofotometri. Validasi dilakukan dengan mengukur linearitas, akurasi, dan kekasaran metode yang memenuhi persyaratan beserta penetapan Limit of Detection (LoD) dan Limit of Quantification (LoQ). Penentuan presisi (berdasarkan %RSD dan CV), 100 ppm katekin dibuat 7 ulangan sedangkan akurasi dievaluasi dengan menghitung perolehan kembali. Kekasaran metode ditentukan dengan menganalisisnya pada hari yang berbeda. Linearitas ditentukan dengan mengukur nilai r pada hubungan antara kurva absorbansi dan konsentrasi seri standar. Berdasarkan persamaan kurva standar, maka LoD dan LoQ dapat dihitung. Hasil penelitian menunjukkan metode memenuhi syarat linearitas dengan R 0,9996; memiliki presisi di 1,23% RSD; memiliki akurasi pada kisaran 100,80% sampai dengan 101,64%; kekasaran metode tidak berbeda nyata; memiliki 3,85 ppm LoD; dan 12,84 ppm LoQ. Validasi menunjukkan hasil yang valid, dan metode tersebut dapat digunakan untuk analisis rutin pada pengukuran katekin dalam fraksi etil asetat ekstrak gambir. Kata kunci: Catechin, Uncaria gambir, Validasi Metode, Spektrofotometri

show abstract

Section: Fractionationmentioning

confidence: 99%

VALIDATION OF SPECTROPHOTOMETRY METHOD FOR DETERMINATION OF (+)-CATECHIN IN ETHYL ACETATE FRACTION OF GAMBIR EXTRACT (Uncaria gambir Roxb.)

Yunarto

Reswandaru

Sulistyowati³

et al. 2021

J. Tumbuhan Obat Indonesia

View full text Add to dashboard Cite

show abstract

“…The mobile phase is used gradiently with the mobile phase A: 0.1% trifluoroacetic acid in a mixture of acetonitrile: water (5:95) and the mobile phase B 0.1% triflouroacetic acid in acetonitrile. The gradient conditions of the mobile phase are 0-4 minutes (100% A) 4-20 minutes (71.5 A; 28.5 B) and 20-30 minutes (100% B) [10].…”

Section: Determination Of Catechin Levelsmentioning

confidence: 99%

Anti-Inflammatory Activities of Ethyl Acetate Fraction From Uncaria Gambir Leaves Through the Inhibition of Edema, COX-2 and iNOS Expression

Yunarto¹,

Intan²,

Kurniatri³

et al. 2020

Proceedings of the 4th International Symposium on Health Research (ISHR 2019)

Self Cite

View full text Add to dashboard Cite

Inflammatory is a body defense mechanism due to respond of tissue on undermine influences, either local or the one that go into the body. Inflammatory can cause various physical dysfunctions. Uncaria gambir Roxb, a high catechin content in ethyl acetate fraction, is widely used as wound healing in Indonesian culture, therefore its mechanism of anti-inflammatory action is still unknown. The objective of our study is to investigated the activity of anti-inflammatory ethyl acetate fraction of Uncaria gambir leaves inhibition of edema, COX-2 and iNOS expression using induction method with carrageenan in rats. This study is experimental research which use 25 white Wistar strain rats as animals assay that divided into 5 groups: negative group (water), positive group (sodium diclofenac 50 mg/kg bw), dose I (5 mg/kg bw fraction), II (10 mg/kg bw fraction) and III (20 mg/kg bw fraction) groups. Each of them were given orally 30 min before carrageenan 3% induced. The paw volume was measured every day for seven day after injection carrageenan using plethysmometer. Inhibition COX-2 and iNOS expression were determined by enzyme-linked immunosorbent assay. The results showed that ethyl acetate fraction of Uncaria gambir leaves in all doses had anti-inflammatory effect on white rats through the mechanism of inflammatory inhibition by reducing edema volume. All three fraction doses can also reduce COX-2 and iNOS expression. The fraction had the highest anti-inflammatory potency by the dose of 20 mg/kg body weight. The conclusion that ethyl acetate of three doses had anti-inflammatory activities through a mechanism of edema volume reduction, COX-2 and iNOS expression.

show abstract

Inhibitory activity of Uncaria Gambir Roxb extract, ethyl acetate fraction, and catechin isolate on lipase

Yunarto

Sulistyowati

Reswandaru

et al. 2023

AIP Conference Proceedings

View full text Add to dashboard Cite

Pengaruh Penyalutan Terhadap Karakteristik Fisika Kimia dan Stabilitas Tablet Fraksi Etil Asetat Daun Gambir sebagai Agen Antidislipidemia

Cited by 3 publications

References 0 publications

VALIDATION OF SPECTROPHOTOMETRY METHOD FOR DETERMINATION OF (+)-CATECHIN IN ETHYL ACETATE FRACTION OF GAMBIR EXTRACT (Uncaria gambir Roxb.)

VALIDATION OF SPECTROPHOTOMETRY METHOD FOR DETERMINATION OF (+)-CATECHIN IN ETHYL ACETATE FRACTION OF GAMBIR EXTRACT (Uncaria gambir Roxb.)

Anti-Inflammatory Activities of Ethyl Acetate Fraction From Uncaria Gambir Leaves Through the Inhibition of Edema, COX-2 and iNOS Expression

Inhibitory activity of Uncaria Gambir Roxb extract, ethyl acetate fraction, and catechin isolate on lipase

Contact Info

Product

Resources

About