Inhibitory activity of Uncaria Gambir Roxb extract, ethyl acetate fraction, and catechin isolate on lipase

Yunarto, Nanang; Sulistyowati, Indah; Reswandaru, Uud Nourma; Elya, Berna; Sauriasari, Rani; Konadi, Laurentia

doi:10.1063/5.0118363

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…1 Gambir is a typical plant produced in four provinces in Indonesia, namely West Sumatra, North Sumatra, Riau, and South Sumatra. 2 However, as much as 90% of the gambir in Indonesia is produced in West Sumatra. The primary component of gambir extract is made up of 28-54% catechins, which are secondary metabolites of the flavonoid class.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a High-Performance Liquid Chromatography-Based Method for Catechin Isolated from the Leaves of Gambir (Uncaria gambir Roxb)

2023

TJNPR

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Gambir (Uncaria gambir Roxb) has long been thought to provide various health benefits due to the main secondary metabolite, catechin. The high catechin content makes gambir a strong antioxidant. The present study was conducted to develop and validate the quantification of catechin in gambir leaves by high-performance liquid chromatography (HPLC). The method was performed using a 100 mL sample with dilution. The separation process employed a Sunfire C18 (150 mm × 4.6 mm) column, the mobile phase was composed of solvent A: 0.03% trifluoroacetic acid in acetonitrile-water (5:95) and solvent B: 0.1% trifluoroacetic acid in acetonitrile at a 0.45 mL/min flow rate. The validation was conducted by measuring the accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness. The results showed that the percent recovery was 98.30-99.82% with a precision value of 0.28% RSD, good linearity with a 0.9996 R-value, 2.7 µg/mL of LOD, and 8.3 µg/mL of LOQ. There was no significant difference between changes in the mobile phase of 4% (comparison of area and retention time), and 6% (comparison of area). However, the results also demonstrated that the mobile phase was not resistant to changes in the mobile phase of 6% in the ratio of retention time because of the shift in retention time that was faster than the catechin standard. The findings of this study reveal that this method satisfies the validation requirements for accuracy, precision, linearity, and robustness tests, therefore, the HPLC-based method can be applied to analyze catechin in gambir leaves.

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Section: Introductionmentioning

confidence: 99%

Development and Validation of a High-Performance Liquid Chromatography-Based Method for Catechin Isolated from the Leaves of Gambir (Uncaria gambir Roxb)

2023

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“…The inhibitory activity of catechins increases with their concentration, lowering total cholesterol levels. 5 The development of raw materials in the form of the ethyl acetate fraction of gambir leaf extract will improve the quality of raw materials due to the high catechin content in the ethyl acetate fraction of gambir (EAFG), cleaner physical appearance, lower water, and ash content. Catechin compounds are less stable in the presence of humidity and water, making them more easily oxidized.…”

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Evaluation of Film-Coated Tablets of Ethyl Acetate Fraction of Uncaria gambir (Hunter) Roxb

2024

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Catechins from gambir leaves have high antioxidant activity. 4 They can inhibit the activity of the HMG-CoA reductase and lipase enzymes. The inhibitory activity of catechins increases with their concentration, lowering total cholesterol levels. 5 The development of raw materials in the form of the ethyl acetate fraction of gambir leaf extract will improve the quality of raw materials due to the high catechin content in the ethyl acetate fraction of gambir (EAFG), cleaner physical appearance, lower water, and ash content. Catechin compounds are less stable in the presence of humidity and water, making them more easily oxidized. 6 Also, catechins are less stable to UV light and visible light. 7 One effort to increase the stability of catechins in EAFG is by formulating them in film-coated tablet dosage forms. Film-coated tablets can increase product stability by containing active substances sensitive to moisture and light and easily oxidized. 8 The present study aimed to produce and evaluate film-coated tablets made from the ethyl acetate fraction of gambir leaves. The quality of film-coated tablets was assessed based on their physicochemical properties, drug release, and stability during storage. Materials and Methods Preparation of gambir leaf extractThe extract of gambir leaves was prepared from plants obtained from Mungka District,

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Inhibitory activity of Uncaria Gambir Roxb extract, ethyl acetate fraction, and catechin isolate on lipase

Cited by 2 publications

References 18 publications

Development and Validation of a High-Performance Liquid Chromatography-Based Method for Catechin Isolated from the Leaves of Gambir (Uncaria gambir Roxb)

Development and Validation of a High-Performance Liquid Chromatography-Based Method for Catechin Isolated from the Leaves of Gambir (Uncaria gambir Roxb)

Evaluation of Film-Coated Tablets of Ethyl Acetate Fraction of Uncaria gambir (Hunter) Roxb

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