PCR‐RFLP and DAMD‐PCR genotyping for <i>Salvia</i> species

Karaca, Mehmet; İnce, Ayşe Gül; Ay, Saadet Tuğrul; Turgut, Kenan; Onus, Ahmet Naci

doi:10.1002/jsfa.3372

Cited by 43 publications

(19 citation statements)

References 31 publications

(55 reference statements)

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“…RFLP has been used to evaluate the variability among species-or genotype-specific sequences (Nalini et al, 2001;Van Droogenbroeck et al, 2004;Karaca et al, 2008). In the present study, RFLP using three restriction enzymes (AluI, RsaI and Sau3AI) revealed distinctive RFLP profiles among the ITS1-5.8S-ITS-2 regions of the three species (Fig.…”

Section: Discussionmentioning

confidence: 91%

“…As a result, various molecular methods such as RAPD (Boehm et al, 1999;Kim and Choi, 2003), AFLP (Kim et al, 2005), RFLP (Karaca et al, 2008), ITS/NTS (Ngan et al, 1999;Cui et al, 2003;Xu et al, 2009), SSR (Kim et al, 2007) and SCAR (Warude et al, 2006;Choi et al, 2007;Choo et al, 2009), have been utilized to authenticate and distinguish medicinal plants from their relatives and adulterants. However, with the exception of, C. officinale (diploid, 2n=2x=22) and L. chuanxiong (triploid, 2n=3x=33), the cytological background of medicinal plants rarely show cytogenetic relationships (To, 1969(To, , 1971.…”

Section: Introductionmentioning

confidence: 98%

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Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Lee

Choi

Sung³

et al. 2010

Genes Genom

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Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha have long been used as Chinese medicinal herbs to treat the same conditions. To elucidate the inter-specific relationships among these species, molecular and molecular cytogenetic analyses were conducted. Cytogenetically, C. officinale and A. polymorpha were found to be diploid with 2n=2x=22, but L. chuanxiong had a triploid chromosome number (2n= 3x=33). FISH analysis revealed that each two pairs of the 45S-and 5S rRNA genes were detected on the different chromosomes of C. officinale, and that triplet of the rRNA genes were localized on the same loci of the homologue in L. chuanxiong. GISH conducted using a genomic DNA probe detected strong cross-hybridization of genomes between C. officinale and L. chuanxiong, while no distinct GISH signal between C. officinale and A. polymorpha was observed. ITS and NTS data also revealed a very high sequence homology (95−96%) between C. officinale and L. chuanxiong. The RFLP profile of the rDNA-ITS enabled us to confirm the genetic distance among species. An effective species-specific SCAR marker for the identification of the wild plant, A. polymorpha, was also developed. Taken together, the results of this study suggest two possibilities; (1) L. chuanxiong originated from C. officinale, or (2) L. Chuanxiong and C. officinale diverged from the same parental plant.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 98%

Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Lee

Choi

Sung³

et al. 2010

Genes Genom

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“…od (rpoB, rbcL, matK and trnH-psbA) (De Mattia et al 2011), combination of chloroplast and nuclear ribosomal DNA sequences and allozyme (Sudarmono and Okada 2008), random amplifi ed polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers (Wang et al 2011, Sepehry-Javan et al 2012, chloroplast DNA regions of rbcL and trnL-F (Walker et al 2004), nrDNA ITS sequences (Zhan et al 2012), PCR-RFLP (Karaca et al 2008) and simple sequence repeats (SSR) markers (Radosavljević et al 2011(Radosavljević et al , 2012. The present study was performed to investigate genetic diversity and morphological variation in six Salvia species with the aim of producing data regarding their inter-and intra-population genetic structure and possible interspecies gene fl ow.…”

mentioning

confidence: 99%

Species delimitation and genetic diversity analysis in Salvia with the use of ISSR molecular markers

Safaei¹,

Sheidai²,

Alijanpoor³

et al. 2016

Acta Botanica Croatica

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-Thirty-nine plant specimens of six Salvia species were collected from different localities of the Alborz mountain region in Iran and studied for morphological and genetic variability and species relationship. Inter simple sequence repeats (ISSR) molecular markers showed a high degree of within-species and interspecifi c genetic variability in Salvia. Analysis of molecular variance and Hickory tests showed significant molecular difference among the studied populations. A principal coordinate analysis plot of morphological characters grouped the species into two distinct groups, supporting their taxonomic treatment. This was partly supported by ISSR networking. The Mantel test did not show a correlation between genetic distance and the geographical distance of the studied populations. STRUCTURE and reticulation analyses revealed some degree of gene fl ow among the species. The present study showed that ISSR molecular markers could be used in Salvia species delimitation along with morphological study.

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“…However, molecular biological data may provide a Deans, 1999 solution to the taxonomic problems both in intraspecific level and among the thyme species. The use of DNA marker techniques was proven to be important in the case of polymorph species with limited reliable taxonomic characters (Karaca et al, 2008;Ince et al, 2009). Echeverrigaray et al (2001) studied the essential oil composition and genetic variability of six commercial Thymus vulgaris cultivars.…”

Section: Essential Oil Properties and Utilizationmentioning

confidence: 99%

Effects of different factors influencing the essential oil properties of Thymus vulgaris L.

2016

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Thymus vulgaris L. is a well-know medicinal and aromatic plant native to the Mediterranean region. The essential oil is considered as the main active constituent, being responsible for its typical odour and taste as well as for several therapeutic effects. Our aim was to demonstrate the most important factors influencing the quality and quantity parameters of thyme oil by summarizing the available literature data and our own scientific results. Genetic background, climatic and growing conditions, techniques of primary processing, storage conditions as well as different extraction methods have proven effects on the essential oil properties and, as a consequence, on its biological activity, either.

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PCR‐RFLP and DAMD‐PCR genotyping for Salvia species

Cited by 43 publications

References 31 publications

Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Species delimitation and genetic diversity analysis in Salvia with the use of ISSR molecular markers

Effects of different factors influencing the essential oil properties of Thymus vulgaris L.

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