Optimisation of plantlet regeneration from leaf and nodal derived callus of Vanilla planifolia Andrews

Abstract

An indirect in vitro plant regeneration protocol for Vanilla planifolia has been established. Juvenile leaf and nodal segments from V. planifolia were used as explants to initiate callus. Nodal explants showed better callus initiation than juvenile leaf explants, with 35.0% of explants forming callus when cultured on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg/l 1-naphthylacetic acid (NAA) and 1.0 mg/l 6-benzyladenine (BA). Almost 10.0% of juvenile leaf explants were induced to form callus on the MS basal medium containing 2.0 mg/l NAA and 2.0 mg/l BA, whereas no callus formed in the presence of any concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and BA. After 8 weeks, callus generated was transferred to MS basal medium containing 1.0 mg/l BA and 0.5 mg/l NAA. A mean number of 4.2 shoots per callus was produced on this medium, with a mean length of 3.8 cm after 8 weeks of culture. Roots formed on 88.3% of plantlets when they were cultured on MS medium supplemented with 1.0 mg/l NAA, with a mean length of 4.4 cm after 4 weeks of culture. Of the rooted plantlets, 90.0% survived acclimatisation and were making new growth after 4 weeks.

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

“…TDZ is also useful for rapid plant regeneration through organogenesis (Kishor & Devi, 2009;Jitsopakul, Thammasiri, & Ishikawa, 2013), somatic em-bryogenesis (Jheng, Do, Liauh, Chung, & Huang, 2006;Gow, Chen, & Chang, 2009), or PLB formation of orchids (Mayer, Stancato, & Appezzato-Da-Glória, 2010;Khoddamzadeh et al, 2011;Roy, Sajeev, Pattanayak, & Deka, 2012). NAA is a synthetic auxin plant growth regulator that is routinely used in in vitro propagation of orchid to promote callus induction such as in Dendrobium (Mei, Danial, Mahmood, & Subramaniam, 2012;Salam, Salam, & Mohanty, 2013), Vanilla (Tan, Chin, & Alderson, 2011), Oncidium (Mayer, Stancato, & AppezzatoDa-Glória, 2010), Phalaenopsis (Khoddamzadeh et al, 2011), and Renanthera (Wu et al, 2012).…”

Enhanced Protocol Development for in vitro Multiplication and Rooting of Vanilla (Vanilla planifolia Andr.) Clone (Van.2/05)

“…Unless stated otherwise, in all cases the culture jars containing the explants were placed randomly in the growth room having an average of 50 -60% relative humidity, temperature of 24-26ºC and 2500 lux of light intensity with 16 hrs duration of light [14].…”

Importancia y desafíos de la conservación de Vanilla spp. (Orquidaceae) en Costa Rica.

“…Vanilla planifolia es cultivada comercialmente por sus frutos (cápsulas) de los cuales se extrae la sustancia aromatizante y saborizante llamada vainillina (George y Ravishankar, 1997;Geetha y Shetty, 2000;Giridhar y Ravishankar, 2004;Pak et al, 2004;Kalimuthu et al, 2006;Havkin-Frenkel y Belanger, 2007;Janarthanam y Seshadri, 2008;Chandran y Puthur, 2009;González-Arnao et al, 2009;Tan et al, 2010;Hailemichael et al, 2012). De los más de 250 compuestos diferentes aislados de los frutos de V. planifolia, la vainillina es el componente principal de las cápsulas fermentadas y es el sabor más ampliamente distribuido en el mundo (Pak et al, 2004;HavkinFrenkel y Belanger, 2007).…”