Vanilla (Vanilla planifolia Andr.) is a multipurpose spice in the world. The crop had been introduced to Ethiopia from Mauritius and it is still under maintenance at the Tepi National Spices Research Center (TNSRC). It is commonly propagated through stem cuttings which could arrests subsequent plant growth and development and serves as an ideal means for the spread of varied diseases. To solve the mentioned problems, no efficient in vitro protocol had so far been developed to propagate this vanilla clone in Ethiopia. Therefore, this study was initiated to address this gap by developing efficient protocol for the enhanced multiplication of this accession (Van.2/05). In this study plant material was taken from TNSRC. The experiments were carried out in Jimma Agricultural Research Center (JARC) at Biotechnology Laboratory. Nodal explants and Murashige and Skoog (MS) (1962), basal medium were used exclusively throughout the experiments. The experiments were laid out in Completely Randomized Design (CRD) with factorial treatment combinations and
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