Cassava (Manihot esculenta Crantz) is one of the major food security crops in Ethiopia. Recently, clean planting materials of improved cassava cultivars are in high demand. A limitation, however, is the low multiplication ratio (1:10) of the crop via conventional methods. Thus, a study was undertaken to develop an efficient in vitro mass propagation protocol for two elite cassava clones, 44/72-NR and 44/72-NW. Combination of different plant growth regulators (PGRs); four concentrations of 6benzylaminopurine (BAP) and kinetin (Kin) on shoot multiplication and that of α-naphthaleneacetic acid (NAA) and BAP each at four concentration combination on root induction were assessed. The experiments were factorial laid out in a completely randomized design (CRD) with PGRs as one-factor and clones as another, replicated five times. Significant (p< 0.05) interaction effects were observed in response to shoot multiplication and root induction treatments within six weeks of culture. Murashige and Skoog (MS) medium containing BAP and Kin each at 0.75 mg/L gave an average of 7.30 shoots per explant than other media combinations. Consecutively, the regenerated cassava shoots produced an average of 6.14 roots within four weeks in a 0.5 mg/L NAA medium and were successfully acclimatized and transferred to field.
No abstract
Krawan, one of the common spices and medicinal plants of Thailand, is conventionally propagated by vegetative means. The technique of micropropagation could be used as a possible means to overcome the problem of shortage of planting materials. Excised and surface disinfested axillary buds were cultured on plant growth regulator-free Murashige and Skoog 1 (MS) basal medium prior to culturing on MS, half-macro nutrient MS, half-nitrogen MS and Schenk and Hildebrandt (SH) media. The highest rate of multiplication was obtained from MS medium. When 0, 5, 10, 15 and 20% v/v coconut water (CW) were added, it was found that 5% CW was the best for shoot growth and development, i.e. shoot number, as well as fresh and dry weights. The effects of 0, 2 and 4 mg/l imazalil (IMA) were simultaneously studied in combination with 0, 0.1, and 0.5 mg/l 6-benzyladenine (BA) or thidiazuron (TDZ). The best media for shoot growth and development was the one with 2 mg/l IMA and 0.5 mg/l TDZ. Rooting was accomplished with a relative ease using a Plant Growth Regulator (PGR)-free MS medium. The in vitro rooted plantlets could be established in the soil with more than 90 percent success after transfer to ex vitro.
Vanilla (Vanilla planifolia Andr.) is a multipurpose spice in the world. The crop had been introduced to Ethiopia from Mauritius and it is still under maintenance at the Tepi National Spices Research Center (TNSRC). It is commonly propagated through stem cuttings which could arrests subsequent plant growth and development and serves as an ideal means for the spread of varied diseases. To solve the mentioned problems, no efficient in vitro protocol had so far been developed to propagate this vanilla clone in Ethiopia. Therefore, this study was initiated to address this gap by developing efficient protocol for the enhanced multiplication of this accession (Van.2/05). In this study plant material was taken from TNSRC. The experiments were carried out in Jimma Agricultural Research Center (JARC) at Biotechnology Laboratory. Nodal explants and Murashige and Skoog (MS) (1962), basal medium were used exclusively throughout the experiments. The experiments were laid out in Completely Randomized Design (CRD) with factorial treatment combinations and Original Research ArticleArticle
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.