Enhanced Protocol Development for in vitro Multiplication and Rooting of Vanilla (Vanilla planifolia Andr.) Clone (Van.2/05)

Abstract

Vanilla (Vanilla planifolia Andr.) is a multipurpose spice in the world. The crop had been introduced to Ethiopia from Mauritius and it is still under maintenance at the Tepi National Spices Research Center (TNSRC). It is commonly propagated through stem cuttings which could arrests subsequent plant growth and development and serves as an ideal means for the spread of varied diseases. To solve the mentioned problems, no efficient in vitro protocol had so far been developed to propagate this vanilla clone in Ethiopia. Therefore, this study was initiated to address this gap by developing efficient protocol for the enhanced multiplication of this accession (Van.2/05). In this study plant material was taken from TNSRC. The experiments were carried out in Jimma Agricultural Research Center (JARC) at Biotechnology Laboratory. Nodal explants and Murashige and Skoog (MS) (1962), basal medium were used exclusively throughout the experiments. The experiments were laid out in Completely Randomized Design (CRD) with factorial treatment combinations and Original Research ArticleArticle

Propagación Masiva Y Formación De Callos Protocórmicos De Vainilla a Partir De Ápices Radicales Vanilla Mass Propagation and Protocormic Callus Formation From Root Tips

“…Se han desarrollado diferentes metodologías de micropropagación para V. planifolia, muchas de estas metodologías están enfocadas a la producción de brotes y PLBs (protocorm like bodies) a partir de ápices, nodos y yemas axilares (Divakaran, et al, 2006;Kalimuthu et al, 2006;Gantait et al, 2009;Zuraida et al, 2013;de Oliveira et al, 2013;Jing et al, 2014;Ramos-Castella et al, 2014;Jadid et al, 2015, Biradar et al, 2016Ayele et al, 2017), por medio de microestacas (Kononowicz & Jarrick, 1984;Geetha & Shetty, 2000), o bien a partir de ápices radicales (Philip & Nainar, 1988ay 1988bPierik, 1990, Zuraida et al, 2013; en tanto que otras metodologías han generado la inducción de callos, en general con porcentajes bajos de formación, como un medio para la reproducción clonal y obtención de plantas de esta especie (Davidonis & Knorr, 1991;Janarthanam & Seshadri, 2008;Tan et al, 2011;Palama et al, 2010). George & Ravishankar (1997) determinaron que la combinación de medio líquido y medio sólido favorece la multiplicación de brotes en V. planifolia.…”