Micropropagation of Vanilla (Vanilla planifolia Andrews) with Modification of Cytokinins

Erawati, Dyah Nuning; Wardati, Irma; Humaida, Siti; Fisdiana, Usken

doi:10.1088/1755-1315/411/1/012009

Cited by 7 publications

(7 citation statements)

References 9 publications

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“…In the case when both 0.5 mg/l BAP and0.1mg/lIBA dose were increased to higher than 0.1 mg/l, the number of shoots decreased significantly (Table 1). In previous in vitro propagation studies it was tested that using BAP is more effective than other types of cytokines (Arinaitwe et al, 2000;Arab et al, 2014;Erawati et al, 2020) and determination of optimal BAP concentration and combination is important (Şahin et al, 2016;Yahya et al, 2019). For example, higher levels of BAP induce callus formation, apical shoots necrosis, decrease in shoot number, and also rosette plant formation in embryo culture (Ahmad et al, 2003;Şahin et al, 2016;Yahya et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Researches on the Reproduction of “Ottoman Strawberry” with Tissue Culture

Doğan¹

2021

alinteri

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Plant tissue culture is widely used as auxiliary methods for vegetative propagation due to give results in a shorter time and to obtain mass plant production than other vegetative propagation methods. The combined effect of BAP and IBA hormones (0.1 mg/l IBA; 0.2 mg/l IBA; 0.3 mg/l IBA; 0.5 mg/l BAP; 0.5 mg/l BAP + 0.1 mg/l IBA; 0.5 mg/l BAP + 0.2 mg/l IBA; 0.5 mg/l BAP + 0.3 mg/l IBA) was studied relative to average number of shoots per explant and root formation (%) of 42-day-old in strawberry cultivar "Ottoman Strawberry" shoot tip cuttings. Our results have shown that concentration of BAP + IBA (0.5+ 0.1 mg/l) in MS medium successfully resulted in the induction of average number of shoots and root formation per explant when compared to other treatment doses and control. Alone concentration of BAP had not significant effect on development of average number of shoots per explant and root formation. Development of average number of shoots per explant and root formation was inhibited as the BAP + IBA concentration increased or decreased except for MS culture medium 5 to 0.5 mg/l BAP and 0.1 mg/l IBA. Consequently, in strawberry cultivar "Ottoman Strawberry" tissue culture, application of alone BAP in the concentrations used is discouraged, while application of BAP + IBA (0.5 + 0.1 mg/l) concentrations is recommended.

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Section: Resultsmentioning

confidence: 99%

Researches on the Reproduction of “Ottoman Strawberry” with Tissue Culture

Doğan¹

2021

alinteri

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“…Pertumbuhan tunas tiap eksplan vanili secara maksimal sebanyak 9 tunas/eksplan diperoleh dengan penambahan zat pengatur tumbuh BAP dan Kinetin pada komposisi 2:1 ppm [8]. Hal berbeda dilaporkan oleh [9] yang menyatakan bahwa penggandaan tunas vanili melalui teknik mikropropagasi hanya dipengaruhi oleh BAP (Benzyl Amino Purine) pada konsentrasi 3 mg/liter dengan rerata 3-4 tunas/eksplan pada 28 hari setelah inokulasi.…”

Section: Pendahuluanunclassified

“…[13] melaporkan bahwa meningkatnya konsentrasi BAP akan meningkatkan jumlah tunas dan menurunkan rata-rata panjang tunas. Pendapat tersebut didukung dengan hasil penelitian [9] semakin banyak tunas yang terbentuk akan mempengaruhi panjang tunas yang lebih pendek karena energi yang dibutuhkan untuk tinggi pucuk lebih banyak digunakan untuk penggandaan tunas.…”

Section: Tinggi Tunas (Cm)unclassified

Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

et al. 2021

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Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

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“…However, Indonesia vanilla cultivation is not optimal due to the limited large-scale supply of seedlings in a short time [5]. In vitro propagation or tissue culture can increase vanilla seedling supply because it is season-independent and requires limited supply of plant tissue as a source of explant, ensures a rapid rate of multiplication, supports producing disease-free plants [6][7], and true to type generations [8].…”

Section: Introductionmentioning

confidence: 99%

“…By increasing BAP concentration in media explants respond differently to each other. BAP must be limited to a certain level that is still responded well by explants because it has the potential to inhibit the multiplication of buds [5]. Kinetin or N6 -furfuryloadenine is one of the biologically active cytokinins [16].…”

Section: Introductionmentioning

confidence: 99%

BAP and Kinetin Application for In Vitro Bud Induction of Vanilla (Vanilla planifolia Andrews.) from Stem Node Explants in the Initiation Stage

Mahardhini,

Anwar,

Karno

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Due to the scarcity of seedlings, vanilla farming in Indonesia is not at its best. The goal of this study was to comprehend how BAP and Kinetin affected the introduction of vanilla buds as a sustainable seedling strategy. The study is carried out at Salatiga, Central Java, Indonesia, at the Plant Tissue Laboratory of the Plantation Seeds Center and the Plant Tissue Laboratory of Diponegoro University’s Physiology and Plant Breeding Laboratory. A completely randomized design (CRD) 5 x 3 with two replications was utilized in the study. The first factor is BAP concentration: B0 = 0 mg.L−1, B1 = 0.6 mg.L−1, B2 = 1.2 mg.L−1, B3 = 1.8 mg.L−1, and B4 = 2.4 mg.L−1. The second factor is Kinetin concentration: K0 = 0 mg.L−1, K1 = 1 mg.L−1, and K2 = 2 mg.L−1. The result shows addition of BAP is able to shorten the day of bud emergence at 8 days. The addition of BAP and Kinetin did not significantly affect the number of buds. Application of BAP 1.44 mg.L−1 was able to increase bud length. There is interaction between Kinetin 2 mg.L−1 and the addition of BAP 2.4 mg.L−1 resulting in the largest bud diameter with a linear regression form. There is interaction between Kinetin 0 mg.L−1 and BAP 1.2 mg.L−1 resulting in the highest number of roots.

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Micropropagation of Vanilla (Vanilla planifolia Andrews) with Modification of Cytokinins

Cited by 7 publications

References 9 publications

Researches on the Reproduction of “Ottoman Strawberry” with Tissue Culture

Researches on the Reproduction of “Ottoman Strawberry” with Tissue Culture

Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

BAP and Kinetin Application for In Vitro Bud Induction of Vanilla (Vanilla planifolia Andrews.) from Stem Node Explants in the Initiation Stage

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