Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

Erawati, Dyah Nuning; Mawaddah, Yusriatul; Humaida, Siti; Wardati, Irma

doi:10.25047/jii.v21i1.2636

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Jumlah tunas pada perlakuan BAP dan Kinetin pada 6 hingga 12 MST Pemberian BAP menghasilkan peningkatan jumlah tunas seiring dengan umur eksplan sedangkan pemberian Kinetin menghasilkan respon yang stabil hingga umur 12 MST. Respon tersebut sesuai dengan hasil penelitian yang dilakukan oleh Erawati et al (2021) dan Rasud et al (2015). Perbedaan jenis sitokinin dapat direspon berbeda oleh eksplan, termasuk dalam pertunasan (Bimantara et al 2018).…”

Section: Jumlah Tunasunclassified

“…Menurut Iliev et al (2010), penambahan sitokinin dapat meningkatkan bobot basah kultur dikarenakan adanya peranan sitokinin yang berguna dalam meningkatkan aktivitas pembelahan sel. Hasil serupa terjadi pada penelitian Erawati et al (2021), yang menunjukkan bahwa perlakuan BAP menghasilkan bobot basah kultur vanili (Vanilla planifolia) yang lebih berat dibandingkan dengan perlakuan Kinetin. Hal yang sama terjadi pada penelitian Amer et al (2013) yang menunjukkan bahwa perlakuan BAP menghasilkan bobot basah kultur yang lebih berat dibandingkan perlakuan Kinetin dalam konsentrasi yang sama pada tanaman stroberi.…”

Section: Bobot Basahunclassified

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Perbandingan Efek Bap Dan Kinetin Terhadap Laju Multiplikasi Stroberi Kultivar Sweet Charlie

Andriani,

Kadapi,

Suminar

et al. 2023

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Seed propagation of strawberry by in vitro culture is needed to obtain high quality seeds of strawberry in large quantities. In in vitro culture, a large number of shoots is expected can be produced at the multiplication stage. Cytokinins type of PGR can be added to culture media to produce a large number of shoot. The focus of this research was to compare the effect of BAP and Kinetin affected Sweet Charlie strawberry multiplication. The experiment was located at the Seed Technology Tissue Culture Laboratory, Faculty of Agriculture, Padjadjaran University. The experimental design was a Completely Randomized Design (CRD) with two treatments of different types of cytokinin, namely Benzyl Amino Purine (BAP) and Kinetin at 1 mg L-1 on Murashige and Skoog (MS) basic media, each treatment was repeated four times. This study showed that BAP had a better effect than Kinetin on the number of shoots, number of leaves, and wet weight of strawberry Sweet Charlie cultivar. The addition of BAP was recommended over Kinetin to obtain the highest number of shoots, the highest number of leaves, and the highest wet weight of culture on strawberry Sweet Charlie cultivar.

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Section: Jumlah Tunasunclassified

Section: Bobot Basahunclassified

Perbandingan Efek Bap Dan Kinetin Terhadap Laju Multiplikasi Stroberi Kultivar Sweet Charlie

Andriani,

Kadapi,

Suminar

et al. 2023

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BAP and Kinetin Application for In Vitro Bud Induction of Vanilla (Vanilla planifolia Andrews.) from Stem Node Explants in the Initiation Stage

Mahardhini,

Anwar,

Karno

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Due to the scarcity of seedlings, vanilla farming in Indonesia is not at its best. The goal of this study was to comprehend how BAP and Kinetin affected the introduction of vanilla buds as a sustainable seedling strategy. The study is carried out at Salatiga, Central Java, Indonesia, at the Plant Tissue Laboratory of the Plantation Seeds Center and the Plant Tissue Laboratory of Diponegoro University’s Physiology and Plant Breeding Laboratory. A completely randomized design (CRD) 5 x 3 with two replications was utilized in the study. The first factor is BAP concentration: B0 = 0 mg.L−1, B1 = 0.6 mg.L−1, B2 = 1.2 mg.L−1, B3 = 1.8 mg.L−1, and B4 = 2.4 mg.L−1. The second factor is Kinetin concentration: K0 = 0 mg.L−1, K1 = 1 mg.L−1, and K2 = 2 mg.L−1. The result shows addition of BAP is able to shorten the day of bud emergence at 8 days. The addition of BAP and Kinetin did not significantly affect the number of buds. Application of BAP 1.44 mg.L−1 was able to increase bud length. There is interaction between Kinetin 2 mg.L−1 and the addition of BAP 2.4 mg.L−1 resulting in the largest bud diameter with a linear regression form. There is interaction between Kinetin 0 mg.L−1 and BAP 1.2 mg.L−1 resulting in the highest number of roots.

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Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

Cited by 2 publications

References 7 publications

Perbandingan Efek Bap Dan Kinetin Terhadap Laju Multiplikasi Stroberi Kultivar Sweet Charlie

Perbandingan Efek Bap Dan Kinetin Terhadap Laju Multiplikasi Stroberi Kultivar Sweet Charlie

BAP and Kinetin Application for In Vitro Bud Induction of Vanilla (Vanilla planifolia Andrews.) from Stem Node Explants in the Initiation Stage

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