Optical Nanomanipulations of Malignant Cells: Controlled Cell Damage and Fusion

Abstract

Specifically targeting and manipulating living cells is a key challenge in biomedicine and in cancer research in particular. Several studies have shown that nanoparticles irradiated by intense lasers are capable of conveying damage to nearby cells for various therapeutic and biological applications. In this work ultrashort laser pulses and gold nanospheres are used for the generation of localized, nanometric disruptions on the membranes of specifically targeted cells. The high structural stability of the nanospheres and the resonance pulse irradiation allow effective means for controlling the induced nanometric effects. The technique is demonstrated by inducing desired death mechanisms in epidermoid carcinoma and Burkitt lymphoma cells, and initiating efficient cell fusion between various cell types. Main advantages of the presented approach include low toxicity, high specificity, and high flexibility in the regulation of cell damage and cell fusion, which would allow it to play an important role in various future clinical and scientific applications.

Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

“…The data shown represent percentage increase compared to control and represents the mean ± standard deviation. Taken together, these data demonstrate that AuNPs induced ROS accumulation is dose and time-dependent in lymphoma cell lines [43]. The earliest intracellular events were occurred in apoptosis due to disruption of the mitochondrial membrane potential.…”

“…That ROS played an important role in apoptosis of lymphoma cell lines SUDHL-4 was demonstrated by near complete abrogation of apoptosis by the antioxidant NAC and by enhancement of apoptosis by the GSH depleting agent BSO. These observations were not restricted to cell lines [43].…”

“…Indeed, we found that the effects of AuNPs, were dose and time related, and were accompanied by ROS generation (Figure 9). That ROS played an important role in apoptosis of lymphoma cell lines SUDHL4 was demonstrated by near complete abrogation of apoptosis by the antioxidant NAC and by enhancement of apoptosis by the GSH depleting agent BSO [43] [45] [46].…”

Deep UV generation and direct DNA photo-interaction by harmonic nanoparticles in labelled samples

“…35,36 To date, NPs-based strategies imply using of organic sensitizers (with some notable exceptions. 37 ) and are mediated by ROS generation. 18,34,38,39 As for classical phototherapy, these approaches can generate major side effects due to the presence of toxic compounds and ROS which can diffuse to nearby tissues generating oxidative stress.…”

Controlled release of Rituximab from gold nanoparticles for phototherapy of malignant cells

“…Pulses in the femtosecond range, which in some conditions may induce multiphoton ionization in addition to rapid heating [44], were reported effective in damaging cancerous HeLa cells conjugated to gold nanorods [59]. Recently, our group has shown that single resonant femtosecond laser pulses in the visible wavelength range may induce well-controlled necrosis, apoptosis and fusion of epidermoid carcinoma cells and Burkitt lymphoma cells targeted by antibody-coated gold nanospheres [60].…”