Deep UV generation and direct DNA photo-interaction by harmonic nanoparticles in labelled samples

Abstract

A novel bio-photonics approach based on the nonlinear optical process of second harmonic generation by non-centrosymmetric nanoparticles is presented and demonstrated on malignant human cell lines. The proposed method allows to directly interact with DNA in absence of photosensitizing molecules, to enable independent imaging and therapeutic modalities switching between the two modes of operation by simply tuning the excitation laser wavelength, and to avoid any risk of spontaneous activation by any natural or artificial light source.

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

“…37 Recently BFO-NP were employed in vitro in a pilot study where they proved to be able to locally induce DNA damages by deep UV generation. 12 The cytotoxicity, haemolytic response and internalization mechanisms evidence here reported for coated BFO-NP suggested good biocompatibility and a great potential Figure 5. Heatmaps tables illustrating toxicity indicated parameters of BFO-NP in human-derived cell lines.…”

“…Here we present a study based on BiFeO 3 (bismuth ferrite, abbreviated as BFO) NP (BFO-NP), which were recently successfully introduced as photodynamic tools and imaging probes. 12 Nonetheless, such is the technological novelty of this new group of materials that there is still a knowledge gap that requires the scientific community attention towards the investigation of the interaction at the cellular and subcellular levels. The opportunity of closing this gap is presented by providing the first thorough investigation on the effects of BFO-NP in cellular metabolism and uptake mechanisms.…”

Harmonic nanoparticles: noncentrosymmetric metal oxides for nonlinear optics

“…In particular, longer wavelengths are less prone to scattering and allow deeper imaging in thick samples [3]. On the other hand, the generation of narrow bandwidth spectra through the ultraviolet and visible region can be used to exert selective interaction with the HNPs surrounding, for example to trigger a specific molecular response [62].…”

Nonlinear optical and magnetic properties of BiFeO3 harmonic nanoparticles

“…26 BFO nanoparticles present a unique combination of potentially high nonlinear optical properties and magnetic characteristics, which make them promising for the targeted bio-imaging applications and possible therapeutic applications. 27 Here, we describe an extensive study on BFO nanoparticles preparation followed by nonlinear optical and magnetic characterization. BFO nanoparticles were produced by an original combustion synthesis with the aim of using a scalable production method and of lowering the annealing temperature.…”