Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

Parida, Umesh Kumar; Biswal, Susanta Kumar; Bindhani, Birendra Kumar

doi:10.4236/abc.2014.46041

Cited by 35 publications

(16 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ROS level in MCF7 and HeLa cells increased after the tiopronin-coated AuNP treatment along with antioxidants [6]. After treated with 60 μm concentration of AuNPs on human SUDHL-4 cell lines, 80% of ROS was increased in a dose-and time-dependent manner [48]. In this study, the effect on ROS generation in the sensitization of mitochondrial membrane potential was determined using rhodamine 123 solution, and the results showed that chemically synthesized AuNPs depolarize the mitochondrial membrane of A549 cells.…”

Section: Discussionmentioning

confidence: 94%

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

et al. 2017

View full text Add to dashboard Cite

The present study reports the chemical synthesis of gold nanoparticles (AuNPs) using sodium borohydride as a reducing and capping agent. The various temperatures and time intervals were investigated for controlled formation of AuNPs. The characterization of AuNPs was studied using UV-vis spectrophotometer, transmission electron microscope (TEM), selected area diffraction (SAED), energy-dispersive X-ray spectrophotometer (Edx), dynamic light scattering (DLS), zeta potential, and X-ray powder diffraction (XRD), and results indicated that AuNPs were spheroid in shape and cubic lattice and face centered in nature with 29 nm in size. AuNPs have potential antimicrobial activity against selected human clinical pathogens. IC 50 concentration of AuNPs was determined as 20 μg/ml against non-small cell lung cancer (NSCLC) cell lines using MTT assay. The morphological and apoptosis effects of AuNPs on NSCLC were investigated using acridine orange/ethidium bromide (AO/EB), 2′,7′-dichlorofluorescein-diacetate (DCFH-DA), rhodamine 123, and 4′,6-diamidino-2-phenylindole (DAPI) staining method, and results indicated that AuNPs have potential anticancer activity against NSCLC cell lines. The anticancer activity of AuNPs is that it has less toxicity against human HBL100 normal cells. These anticancer potentials of AuNPs were compared with the standard drug cisplatin. Moreover, after 24 h exposure, AuNPs arrest the cell cycle at G2/M phase in A549 cells. These findings suggested that AuNPs could act as a potential anticancer agent against lung cancer.

show abstract

Section: Discussionmentioning

confidence: 94%

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The morphological hallmarks which are nuclear material fragmentation and condensation indicate the induction of apoptosis which leads to cell death [29][30][31]. The inhibition of cell growth occurred due to cell cycle arrest, cell permeability interruption, reactive oxygen species production and activation of caspase cascade-mediated apoptosis [1,29,32,33].…”

Section: Resultsmentioning

confidence: 99%

Anticancer activity of Sasa borealis leaf extract-mediated gold nanoparticles

Patil

Jin

Simeon

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Advancements in metal nanoparticle synthesis using plant extracts and their anticancer activity have received significant attention in recent years. The green approach for the synthesis of gold nanoparticles (AuNPs) using leaf extract of Sasa borealis is reported in this study. Synthesis of AuNPs was performed at 50 °C, and nanoparticle formation was observed after 20 min incubation. AuNPs formation was confirmed by the UV-visible spectrum peak at 542 nm. The synthesized AuNPs were oval, spherical with sizes around 10-30 nm observed using the transmission electron microscope. Energy dispersive X-ray analysis was utilized for the detection of elemental compound. The face centered cubic structure was confirmed by X-ray diffraction pattern. The reduction of tetrachloroauric acid into AuNPs by the phytochemical compounds of S. borealis extract was determined by Fourier transform infrared spectroscopy and the presence of biomolecules was studied by GC-MS. The synthesized AuNPs was tested for toxic effect on HEK293 cells and anticancer activity on AGS cells by WST-1 assay. Condensation or fragmentation is a characteristics of apoptosis, which was confirmed by 4,6-diamidino-2-pheynylindole dihydrochoride (DAPI) staining. The S. borealis-mediated AuNPs have good activity as an anticancer agent and it will be beneficial in cancer therapeutics.

show abstract

“…It exhibits wide pharmacological properties such as diuretic, antihypertensive, antidiabetic, anticancer and anti-inflammatory properties23. Previously, extract of this plant has been used for synthesis of silver NPs (~4–17 nm) and gold NPs (~12–20 nm)242526.…”

mentioning

confidence: 99%

Biogenic synthesis of Zinc oxide nanostructures from Nigella sativa seed: Prospective role as food packaging material inhibiting broad-spectrum quorum sensing and biofilm

Al‐Shabib

Husain

Ahmed

et al. 2016

Sci Rep

133

View full text Add to dashboard Cite

Bacterial spoilage of food products is regulated by density dependent communication system called quorum sensing (QS). QS control biofilm formation in numerous food pathogens and Biofilms formed on food surfaces act as carriers of bacterial contamination leading to spoilage of food and health hazards. Agents inhibiting or interfering with bacterial QS and biofilm are gaining importance as a novel class of next-generation food preservatives/packaging material. In the present study, Zinc nanostructures were synthesised using Nigella sativa seed extract (NS-ZnNPs). Synthesized nanostructures were characterized hexagonal wurtzite structure of size ~24 nm by UV-visible, XRD, FTIR and TEM. NS-ZnNPs demonstrated broad-spectrum QS inhibition in C. violaceum and P. aeruginosa biosensor strains. Synthesized nanostructures inhibited QS regulated functions of C. violaceum CVO26 (violacein) and elastase, protease, pyocyanin and alginate production in PAO1 significantly. NS-ZnNPs at sub-inhibitory concentrations inhibited the biofilm formation of four-food pathogens viz. C. violaceum 12472, PAO1, L. monocytogenes, E. coli. Moreover, NS-ZnNPs was found effective in inhibiting pre-formed mature biofilms of the four pathogens. Therefore, the broad-spectrum inhibition of QS and biofilm by biogenic Zinc oxide nanoparticles and it is envisaged that these nontoxic bioactive nanostructures can be used as food packaging material and/or as food preservative.

show abstract

Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

Cited by 35 publications

References 46 publications

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

Anticancer activity of Sasa borealis leaf extract-mediated gold nanoparticles

Biogenic synthesis of Zinc oxide nanostructures from Nigella sativa seed: Prospective role as food packaging material inhibiting broad-spectrum quorum sensing and biofilm

Contact Info

Product

Resources

About