Oppositely Charged Pickering Emulsion Co-Stabilized by Chitin Nanoparticles and Fucoidan: Influence of Environmental Stresses on Stability and Antioxidant Activity

Abstract

Single emulsifiers exhibit varying degrees of restriction in stabilizing emulsions. Oppositely charged chitin nanoparticles and fucoidan complex particles were used as emulsifiers to stabilize a o/w Pickering emulsion and explore its stability and antioxidant activity under different environmental stresses. The results showed that the emulsion with the smallest mean particle size (1.02 μm) and strongest zeta potential (−29.3 mV) was formed at pH 7. Moreover, at this pH, it presented the highest physical stability and antioxidant activity and the lowest emulsion creaming index. The investigation of the effect of temperature on the stability and antioxidant activity of the emulsion revealed that, after freezing/thawing at −20 °C, the emulsion was unstable, the particle size increased, and the stability and antioxidant activity were low. In contrast, the emulsions treated at 25, 37, and 60 °C displayed no significant differences and exhibited high stabilities and antioxidant activities. Additionally, increasing the salt ion concentration further decreased the emulsion stability and antioxidant activity. Particularly, the emulsion with a salt concentration of 500 mM displayed the lowest stability, and stratification occurred after 30 d of storage. The Pickering emulsion remained stable under different environmental stresses expect for at a temperature of −20 °C and 500 mM salt ion concentration.

Application of Pistacia atlantica Pickering emulsion‐filled chitosan gel for targeted delivery of curcumin

“…The absorbance was recorded by a UV–vis spectrophotometer at 517 nm. The antioxidant activity of samples was calculated according to Equation 2 (Hu et al., 2022):…”

“…The absorbance was recorded by a UV–vis spectrophotometer at 517 nm. The antioxidant activity of samples was calculated according to Equation 2 (Hu et al., 2022 ): …”

Enhancement of infrared absorption through a patterned thin film of magnetic field and spin-coating directed self-assembly of gold nanoparticle stabilised ferrofluid emulsion

“…Based on observation that the ferrouid emulsion was solely stabilized through the adsorption of gold nanoparticles, it can be assumed that the overall charge depended on the number of NPs attached to the surface of the ferrouid droplets. 54 Furthermore, the negative zeta potential of PEG-C-GM-pi-FF in the low-zeta potential PVA aqueous solution conrmed the adsorption of gold nanoparticles on the oil droplet. Additionally, the oil droplets were large enough to reduce the overall surface area and zeta potential.…”

Noncovalent interactions between biomolecules facilitated their application in food emulsions' construction: A review

“…Combining oppositely charged NCh and fucoidan could stabilize Pickering emulsions by forming composite particles. These emulsions exhibited smaller droplets, higher electronegativity, and a more uniform particle size distribution under neutral conditions (Hu et al., 2022). Additionally, NCh could form complexes with CNC through electrostatic interaction.…”