One-pot production of maltoheptaose (DP7) from starch by sequential addition of cyclodextrin glucotransferase and cyclomaltodextrinase

Abdalla, Mohammed; Jiang, Bo; Hassanin, Hinawi A.M.; Zheng, Luhua; Chen, Jingjing

doi:10.1016/j.enzmictec.2021.109847

Cited by 12 publications

(5 citation statements)

References 32 publications

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“…49,50 Further hydrolysis results in smaller molecules, mainly maltotriose, maltose, and glucose, which are eventually absorbed and metabolised into CO 2 and H 2 O. [51][52][53][54][55] Known CD-hydrolysing enzymes include cyclomaltodextrinases and a-amylases. 49,50 Chemical conditions, including low pH with Brønsted acids, or the use of Lewis acids, were also reported to catalyse the hydrolytic ring-opening of CDs.…”

Section: Small-animal Pet Imaging and Biodistribution Studiesmentioning

confidence: 99%

A rotaxane-based platform for tailoring the pharmacokinetics of cancer-targeted radiotracers

d’Orchymont

Holland

2022

Chem. Sci.

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Section: Small-animal Pet Imaging and Biodistribution Studiesmentioning

confidence: 99%

A rotaxane-based platform for tailoring the pharmacokinetics of cancer-targeted radiotracers

d’Orchymont

Holland

2022

Chem. Sci.

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“…In fact, β-CD has resistance to gastric acid or saliva and pancreatic amylase, and it is widely hydrolyzed in the colon. β-CD is a cyclic oligosaccharide with non-reducing properties consisting of glucose units connected by α-1,4-glycosidic bonds [ 32 ], and a ring-opening reaction may occur in primary metabolites and acyclic maltodextrin, becoming linked by an α-1,4-glycosidic bond [ 33 ]. The metabolic results showed that di-oxidation and fetone formation occurred in kidney and plasma samples, and I metabolism occurred.…”

Section: Resultsmentioning

confidence: 99%

The Biological Fate of Pharmaceutical Excipient β-Cyclodextrin: Pharmacokinetics, Tissue Distribution, Excretion, and Metabolism of β-Cyclodextrin in Rats

Jiang

Wang

et al. 2022

Molecules

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β-cyclodextrin has a unique annular hollow ultrastructure that allows encapsulation of various poorly water-soluble drugs in the resulting cavity, thereby increasing drug stability. As a bioactive molecule, the metabolism of β-cyclodextrin is mainly completed by the flora in the colon, which can interact with API. In this study, understanding the in vivo fate of β-cyclodextrin, a LC-MS/MS method was developed to facilitate simultaneous quantitative analysis of pharmaceutical excipient β-cyclodextrin and API dextromethorphan hydrobromide. The established method had been effectively used to study the pharmacokinetics, tissue distribution, excretion, and metabolism of β-cyclodextrin after oral administration in rats. Results showed that β-cyclodextrin was almost wholly removed from rat plasma within 36 h, and high concentrations of β-cyclodextrin distributed hastily to organs with increased blood flow velocities such as the spleen, liver, and kidney after administration. The excretion of intact β-cyclodextrin to urine and feces was lower than the administration dose. It can be speculated that β-cyclodextrin metabolized to maltodextrin, which was further metabolized, absorbed, and eventually discharged in the form of CO2 and H2O. Results proved that β-cyclodextrin, with relative low accumulation in the body, had good safety. The results will assist further study of the design and safety evaluation of adjuvant β-cyclodextrin and promote its clinical development.

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“…The starch-processing industry has exploited amylase as a substitute for acid hydrolysis in producing starch hydrolysis. Amylase acts as a biocatalyst for the hydrolysis of starch into simpler carbohydrates, such as glucose, maltose, and dextrin (Divakaran et al 2011;Abdalla et al 2021). Amylolytic bacteria are producers of amylase that can be used as biocatalysts in the starch hydrolysis process (Ding et al 2021) to produce various maltooligosaccharide products, such as maltotriose, maltotetraose, maltopentaose, and maltohexaose (Pan et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase

Agustiani,

Oedjijono,

Rahmani

et al. 2023

J.Tropical Biodiversity Biotechnology

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The objectives of the study were to isolate amylolytic bacteria from the rhizosphere and plant tissue of Canna edulis Ker., as well as litter; to know oligosaccharide compounds produced from starch hydrolyzed by the bacterial enzymes, and to identify the amylolytic bacteria based on phenetic and 16S rRNA gene sequences. From the rhizosphere, Canna plant tissue, and litters obtained thirty-two amylolytic bacterial isolates. Eight isolates (TH6, TH7, T5, T10, D2, D3, A3, S1) produced high clear zone diameters ranging from 18-30 mm; especially an isolate T10, which was consistent in producing a total clear zone diameter of 20 mm. The hydrolysate of starch hydrolysed by the T10 amylase resulted in three oligosaccharide compounds maltotriose, maltotetraose, and maltopentose. The amylase activity of isolate T10 was optimal at a temperature of 40°C and pH at 0.801 U/mL. The isolate T10 was identified as a species member of Bacillus toyonensis based on phenotyphic characterization and 16S rDNA gene sequencing analysis with a similarity value of 99.93%

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One-pot production of maltoheptaose (DP7) from starch by sequential addition of cyclodextrin glucotransferase and cyclomaltodextrinase

Cited by 12 publications

References 32 publications

A rotaxane-based platform for tailoring the pharmacokinetics of cancer-targeted radiotracers

A rotaxane-based platform for tailoring the pharmacokinetics of cancer-targeted radiotracers

The Biological Fate of Pharmaceutical Excipient β-Cyclodextrin: Pharmacokinetics, Tissue Distribution, Excretion, and Metabolism of β-Cyclodextrin in Rats

Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase

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