Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase

Abstract

The objectives of the study were to isolate amylolytic bacteria from the rhizosphere and plant tissue of Canna edulis Ker., as well as litter; to know oligosaccharide compounds produced from starch hydrolyzed by the bacterial enzymes, and to identify the amylolytic bacteria based on phenetic and 16S rRNA gene sequences. From the rhizosphere, Canna plant tissue, and litters obtained thirty-two amylolytic bacterial isolates. Eight isolates (TH6, TH7, T5, T10, D2, D3, A3, S1) produced high clear zone diameters ranging from 18-30 mm; especially an isolate T10, which was consistent in producing a total clear zone diameter of 20 mm. The hydrolysate of starch hydrolysed by the T10 amylase resulted in three oligosaccharide compounds maltotriose, maltotetraose, and maltopentose. The amylase activity of isolate T10 was optimal at a temperature of 40°C and pH at 0.801 U/mL. The isolate T10 was identified as a species member of Bacillus toyonensis based on phenotyphic characterization and 16S rDNA gene sequencing analysis with a similarity value of 99.93%

Identification, Characterization, and Production Optimization of 6-Methoxy-1H-Indole-2-Carboxylic Acid Antifungal Metabolite Produced by Bacillus toyonensis Isolate OQ071612

“…It has enormous economic significance; for example, Bacillus toyonensis BCT-7112 spores have been utilized as probiotic supplements in animal nutrition [15]. It was previously reported to exhibit plant growth promotion, biodegradation, and probiotic and biocontrol properties [16][17][18]. Its biocontrol properties are rarely reported; however, Wang et al reported its toyoncin-producing ability.…”