On Stem‐Cell Recovery after Irradiation

Porteous, D D; Lajtha, L. G.

doi:10.1111/j.1365-2141.1966.tb05623.x

Cited by 74 publications

(7 citation statements)

References 17 publications

(8 reference statements)

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“…These results suggest that the in vitro colony-forming cells could be members of the population of larger cells in the bone marrow, a conclusion in agreement with recent results of Fox and Lajtha (personal communication) indicating that in vitro colony-forming cells are dividing cells in vivo. Porteous and Lajtha (1966) followed the recovery of the repopulating capacity of femur and spleen cells (in vivo colony-forming cells) following a dose of 150 rads. Regeneration in the bone marrow and spleen followed the same pattern although at any one time the femur had a greater repopulating capacity.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Whole Body Irradiation on Serum Colony Stimulating Factor and in Vitro Colony‐Forming Cells in the Bone Marrow

Hall

1969

Br J Haematol

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Summary. Using in vitro cultures of mouse bone marrow cells, levels of colony stimulating factor were assayed in serum from mice following whole body irradiation. No significant differences from control levels were noted in the period 1–32 days following 50, 150, 250 or 450 rads. A dose of 250 rads caused a sharp fall in the level of colony‐forming cells in the bone marrow followed by regeneration between 6 and 16 days following irradiation. Six to 8 hours following whole body irradiation, blood polymorphs and serum colony stimulating factor levels were consistently elevated. Splenectomy did not affect this response. The results suggest that serum colony stimulating factor is unlikely to be the major regulator determining regeneration of in vitro colony‐forming cells in the bone marrow following irradiation.

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Section: Discussionmentioning

confidence: 99%

The Effects of Whole Body Irradiation on Serum Colony Stimulating Factor and in Vitro Colony‐Forming Cells in the Bone Marrow

Hall

1969

Br J Haematol

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“…Our experiments have concentrated on the initial 24 h after irradiation. Most previous studies [3,8,11] have primarily examined longer post-irradiation time spans. We found a slight initial rise in survival if the cells were maintained in situ for one to 2 h post-irradiation.…”

Section: Discussionmentioning

confidence: 99%

Evidence for the repair of potentially lethal damage in irradiated bone marrow

Thomas

Gould

1982

Radiat Environ Biophys

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The effects on cell survival of maintaining bone marrow cells (CFU-S) in situ following irradiation and before assay by transplantation was investigated. When the CFU-S cells are maintained in situ following irradiation survival drops and plateaus at about 9 h post-irradiation. Evidence is presented that this decrease in survival may be due to potentially lethal damage repair (PLD) inhibition caused by post-irradiation in situ holding. This effect on PLD repair is different than that usually found in cells in vitro and in vivo tumors in that it mainly alters the shoulder rather than the slope of the survival curve of CFU-S cells. It is different than PLDR found in vivo for normal mammary and thyroid gland epithelial cells because in situ holding decreases rather than increases the survival of CFU-S cells. Evidence is also presented that the radiation survival curve for in situ bone marrow cells (CFU-S) may not have a shoulder.

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“…PHA was given to groups of animals at various times so that at the time of sacrifice all donors were on the 7th day of fasting but at 1, 2, 3 or 4 days from the time of PH injection. Cell suspension were made as de scribed by Porteous and Lajtha [15] and the cell concentration adjusted so that 105 bone marrow cells or 10« spleen cells were contained in 0.05 ml and these cell doses were used in all cases. An aplastic hemopoietic condition in the recipients was induced by the administration of 350 ml/kg of Cyclophosphamide (Endoxan, ASTA) and 50 ml/kg of Busulfan (Myleran, Borroughs Wellcome & Co.) as de scribed by Santos and H aghshenass [18].…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, the increase of the response to EP caused by PHA pretreatment may be considered to reflect an increased number of stem cells in the splenic compartment, whether they are the pluripotential hematopoietic precur sors [15] or the erythroid committed precursors [2,17,21]. If this were the case then one would expect a similar increase in the concentration of erythroid precursors in the marrow.…”

mentioning

confidence: 98%

Phytohemagglutinin and Hematopoietic Stem Cells in the Mouse

Scaro¹,

Carrera²,

Teruel³

et al. 1971

Acta Haematol

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Administration of phytohemagglutinin to protein-fasted and polycythemic mice results in a striking increase of spleen response to erythropoietin. An opposite ef- s feet was found in bone marrow. In transplant experiments, treatment of prospective cells donors with phytohemagglutinin produces a substantial increase of the cloning efficiency of the spleen cells with little if any effect on bone marrow cells. Results of this study also demonstrate a good correlation between the time course of cytomorphological changes induced by phytohemagglutinin on spleen lymphocytes and increase of hemopoietic stem cell concentration in this organ.

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On Stem‐Cell Recovery after Irradiation

Cited by 74 publications

References 17 publications

The Effects of Whole Body Irradiation on Serum Colony Stimulating Factor and in Vitro Colony‐Forming Cells in the Bone Marrow

The Effects of Whole Body Irradiation on Serum Colony Stimulating Factor and in Vitro Colony‐Forming Cells in the Bone Marrow

Evidence for the repair of potentially lethal damage in irradiated bone marrow

Phytohemagglutinin and Hematopoietic Stem Cells in the Mouse

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