The Effects of Whole Body Irradiation on Serum Colony Stimulating Factor and <i>in Vitro</i> Colony‐Forming Cells in the Bone Marrow

Hall, Barbara

doi:10.1111/j.1365-2141.1969.tb01406.x

Cited by 39 publications

(7 citation statements)

References 10 publications

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“…The present experiments have confirmed earlier observations that the progenitor cells of granulocytes and macrophages (in uitro colony-forming cells, CFCs) are radiosensitive and that following a relatively low dose of 250 rads, regeneration of in vitro CFCs occurs over the subsequent 1 4 1 5 day period (Hall, 1969;Chen & Schooley, 1970;Rickard rt al., 1971). The present experiments have shown that paralleling these changes in in iiitro CFC levels are changes in the levels of colony-stimulating factor (CSF) and serum CSF-inhibitorstwo factors believed to play a role in the normal control of granulopoiesis and macrophage formation.…”

Section: Discussionsupporting

confidence: 91%

Local and Systemic Control of Granulocytic and Macrophage Progenitor Cell Regeneration After Irradiation

Chan¹,

Metcalf²

1973

Cell Proliferation

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Agar cultures of C57BL bone marrow cells were used to determine colony stimulating factor (CSF) and serum CSF‐inhibitor levels in C57BL and BALB/c mice following irradiation. Whole‐body irradiation caused an acute, dose‐dependent, rise in serum CSF levels and fall in CSF‐inhibitor levels. The regeneration of granulocytic and macrophage progenitor cells (in vitro CFCs) in the femur after 250 rads whole‐body irradiation was preceded or paralleled by a fall in serum CSF‐inhibitors and a dramatic rise in the capacity of bone‐adherent cells in the marrow (‘stromal cells’) to produce material with colony‐stimulating activity. No comparable changes were observed in the activity of marrow haemopoietic cells during regeneration or in the lungs or spleen. A similar rise in the activity of bone‐adherent cells was observed in shielded femurs during regeneration of in vitro CFCs. Regeneration of granulocytic and macrophage progenitor cells following irradiation may be regulated by fluctuations in circulating CSF‐inhibitor levels and local production of CSF within the marrow cavity.

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Section: Discussionsupporting

confidence: 91%

Local and Systemic Control of Granulocytic and Macrophage Progenitor Cell Regeneration After Irradiation

Chan¹,

Metcalf²

1973

Cell Proliferation

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“…There is indeed a real possibility that circulating CSF levels rise following extensive eell breakdown. In this context, a quantitative relationship has been documented between the dose of whole-body irradiation administered to mice and the degree of elevation of serum CSF levels during the immediate postirradiation period (Hall, 1969). CSF may well be located at or near the eell membrane and be released in larger than normal amounts following cellular injury or breakdown.…”

mentioning

confidence: 99%

The Colony Stimulating Factor (Csf)

Metcalf¹

1973

Humoral Control of Growth and Differentiation

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“…In addition to the possibility that committed CFU-M gave rise to many of the very early granulocytic colonies (see above), an early development of granulocytic colonies may be due to an early increase in systemic levels of granulocytic colony stimulating factors (CSF) induced by radiation. This is supported by the demonstration of Morley and co-workers (1971) and Hall (1969) that plasma undergoes a rapid increase in its ability to stimulate granulocytic colonies in vitro that is sustained for several days after x-irradiation.…”

Section: Hematopoietic Colony Frequency With Timementioning

confidence: 68%

Studies on the organization and regeneration of bone marrow: Origin, growth, and differentiation of endocloned hematopoietic colonies

Lambertsen

Weiss

1983

Am. J. Anat.

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Hematopoietic colonies were studied by light microscopy in the marrow of alternate fraction x-irradiated mice (C576J/B1) to investigate the microenvironmental organization of marrow and identify early hematopoietic cell-stromal cell interactions. Undifferentiated colonies (UC) were detected at 3 days postirradiation, showed a marked predilection for bone surfaces, and disappeared as differentiated colonies developed. Some UC occurred along marrow arteries. Neutrophilic granulocyte colonies (GC) occurred in all areas at 3 days but grew rapidly only subosteally. Few eosinophilic colonies (GCe) occurred. Erythrocytic colonies (EC) appeared at 4 days as dispersed populations of motile cells within a localized area of marrow; these tended to proliferate initially in intermediate and central marrow zones. Macrophage colonies (M phi C) of two "subtypes" were detected, peaking in relative frequency at 4 days. These appeared active in stromal repair and monocytopoiesis. Megakaryocyte colonies (MC) originated along bone and differentiated away from bone. From 3-5 days, the frequency of GC greater than UC greater than M phi C much greater than MC approximately equal to GCe. All colony types except UC, M phi C, and central GC increased in size and became mixed in differentiation by 12-14 days. For several weeks, however, erythropoiesis concentrated toward central areas, whereas granulopoiesis and thrombopoiesis concentrated along bone. Some mixed colonies showed an abrupt transition from erythrocytic, centrally, to granulocytic, subosteally. These results were interpreted as evidence that in x-irradiated marrow: (1) hematopoietic microenvironments (HMs) for stem-cell proliferation and commitment to differentiation, with the possible exception of HMs determining erythroid differentiation, occur in endosteal and periarterial regions; (2) a proliferative and/or chemotactic stimulus to erythroid progenitors exists in intermediate and central marrow regions; and (3) some subosteal regions may exclude erythropoiesis, or preferentially support nonerythroid differentiation. Elaborate associations occurred between macrophages and early UC, GC, and EC, but not MC hematopoietic cells. UC and GC often associated with osteoclasts. Reticular and other fibroblastic cells associated with the cells of all colony types.

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The Effects of Whole Body Irradiation on Serum Colony Stimulating Factor and in Vitro Colony‐Forming Cells in the Bone Marrow

Cited by 39 publications

References 10 publications

Local and Systemic Control of Granulocytic and Macrophage Progenitor Cell Regeneration After Irradiation

Local and Systemic Control of Granulocytic and Macrophage Progenitor Cell Regeneration After Irradiation

The Colony Stimulating Factor (Csf)

Studies on the organization and regeneration of bone marrow: Origin, growth, and differentiation of endocloned hematopoietic colonies

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