Evidence for the repair of potentially lethal damage in irradiated bone marrow

Thomas, Frank J.; Gould, Michael N.

doi:10.1007/bf01323930

Cited by 8 publications

(2 citation statements)

References 10 publications

(16 reference statements)

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“…1975 and was observed in nondividing confluent fibroblast cultures (Cox et al 1981) . The only rapidly proliferating normal cells that have been examined for ISR are mouse bone marrow spleen colony forming cells (Thomas and Gould 1982) . In the latter system, cell survival was slightly increased if marrow removal for transplantation was delayed from immediately following irradiation to 1-2 hours after exposure .…”

Section: Discussionmentioning

confidence: 99%

The Kinetics ofin SituRepair in Rat Mammary Cells

Mahler

Gould

Clifton

1983

International Journal of Radiation Biology and Related Studies

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The kinetics of in situ repair (ISR) was investigated in normal rat mammary epithelial cells exposed in vivo to 8 Gy 137 Cs gamma rays. ISR as measured by transplantation assay was completed by 4 hours after exposure. Although ISR in the three normal epithelial cell types thus far tested differs from repair of potentially lethal damage in tumour cells and cultured fibroblasts in that it affects the shoulder and not the slope of the survival curve, the current study shows that the time course of ISR resembles that for repair of potentially lethal damage in both tumours and fibroblasts.

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Section: Discussionmentioning

confidence: 99%

The Kinetics ofin SituRepair in Rat Mammary Cells

Mahler

Gould

Clifton

1983

International Journal of Radiation Biology and Related Studies

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“…1984a, b, Gould et al 1984 . In one case, however, inhibition of PLD repair was observed after post irradiation in situ holding of irradiated bone marrow (Thomas and Gould 1982) .…”

Section: Repair and Fixation Of Pld May Affect Survival Of Cells Irmentioning

confidence: 96%

Radiation-induced Potentially Lethal Damage: DNA Lesions Susceptible to Fixation

Iliakis

1988

International Journal of Radiation Biology

131

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The various postirradiation incubation conditions reported to uncover potentially lethal damage (PLD) induced by ionizing radiation are outlined and critically discussed. The process of damage fixation is the most characteristic determinant in distinguishing between PLD and other forms of damage (lethal or non-lethal). The results compiled indicate the induction of two forms of PLD (termed alpha- and beta-PLD). Evidence is presented that repair and fixation of alpha-PLD may underlie the variation in radiosensitivity observed through the cycle. Beta-PLD appears to be sensitive only to postirradiation treatment in anisotonic sale solutions. Results obtained at the DNA and chromosome level, under conditions allowing repair or causing fixation of PLD, are reviewed and combined together to devise a qualitative model that outlines a possible sequence of events from damage fixation at the DNA level, to damage fixation at the chromosome level and, ultimately, to cell death. It is suggested that damage uncovered at the cellular level as potentially lethal, comprises DNA dsb (single, pairs or groups) and that fixation is mediated by forces transmitted to the double helix through alteration (local or general) in chromatin conformation. Changes in chromatin conformation are caused either as a result of the cell's progression through the cycle or in response to a postirradiation treatment. The fixation process leads to the induction of chromosome aberrations. The validity of the concept of PLD in in vivo systems is shown, and the possible importance of PLD repair in radiation therapy is reviewed. The concept of PLD is compared to the concept of sublethal damage, and the possibility that similar molecular lesions underlie both types of damage is discussed.

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Bone Marrow

Nothdurft

1991

Medical Radiology

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Evidence for the repair of potentially lethal damage in irradiated bone marrow

Cited by 8 publications

References 10 publications

The Kinetics ofin SituRepair in Rat Mammary Cells

The Kinetics ofin SituRepair in Rat Mammary Cells

Radiation-induced Potentially Lethal Damage: DNA Lesions Susceptible to Fixation

Bone Marrow

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