Nuclear changes in tongue epithelial cells following panoramic radiography

Silva, Ana Elisa da; Rados, Pantelis Varvaki; Lauxen, Isabel da Silva; Gedoz, Luhana; Villarinho, Eduardo Aydos; Fontanella, Vania Regina Camargo

doi:10.1016/j.mrgentox.2007.05.003

Cited by 34 publications

(43 citation statements)

References 15 publications

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“…Therefore, potential differences between the first and the second counts can be attributed to radiation. 17 The MN assay is a measure of DNA damage. 9,16,28 The MNC frequencies were not significantly different before and after X-ray exposure in our sample.…”

Section: Discussionmentioning

confidence: 99%

“…However, despite higher radiation doses in our investigation, research with similar methodology with dental radiographs showed similar results compared to our study (ie, no mutagenic characteristic was evidenced by the MN test). 6,11,13,14,[17][18][19][20][21] Differences in radiation dose, frequency of exposition, type of cells evaluated, and site of collected cells may influence the results of the MN test. Some authors investigated patients undergoing radiotherapy five to six times per week over the course of 5-7-weeks 16,29 and others observed the effects of frequent occupational exposition to low doses of X-ray in radiation workers, 2,30 whereas some pointed out the results of only a single dental radiograph exposition.…”

Section: Discussionmentioning

confidence: 99%

“…Some authors investigated patients undergoing radiotherapy five to six times per week over the course of 5-7-weeks 16,29 and others observed the effects of frequent occupational exposition to low doses of X-ray in radiation workers, 2,30 whereas some pointed out the results of only a single dental radiograph exposition. 6,13,[17][18][19][20][21] The literature shows that MN-MNC 31 and cellular death 17 increase with radiation dose. With regard to the different cells employed in the MN assay, radiotherapy is shown to be a potent clastogenic agent in circulating lymphocytes and BEC of head-and-neck cancer patients, 16 but lymphocytes are more sensitive in detecting chromosome aberrations caused by anticancer drugs than are BEC.…”

Section: Discussionmentioning

confidence: 99%

“…With regard to the different cells employed in the MN assay, radiotherapy is shown to be a potent clastogenic agent in circulating lymphocytes and BEC of head-and-neck cancer patients, 16 but lymphocytes are more sensitive in detecting chromosome aberrations caused by anticancer drugs than are BEC. 25,28 Additionally, in the cytogenetic studies of dental radiograph effects, different sites were elected from which to collect buccal cells: buccal cheek mucosa, 11,13,14,21 the lateral border of the tongue, 17,21 and keratinized mucosa of the upper dental arch. 12 One of these studies 21 showed that the lateral border of the tongue is more sensitive to cytotoxic insult than is the cheek buccal mucosa.…”

Section: Discussionmentioning

confidence: 99%

“…9 Micronuclei arise from acentric fragments or whole chromosomes that are not included in the main nuclei of the daughter cells and can be induced by agents that cause chromosome breakage or affect the spindle apparatus 10 and can be used as a biomarker in buccal cells to evaluate the mutagenic effect of many agents, including ionizing radiation. 6,[11][12][13][14][15][16][17][18][19][20][21] Previous studies conducted by our research group have demonstrated that the panoramic X-ray is able to induce damage in the oral mucosa cells of children. 13 In this regard, it would be interesting to know if, and to what extent, children submitted to orthodontic radiographic protocol are a more sensitive group as a result of the use of X-ray in oral mucosa cells, particularly because there are no previous reports.…”

Section: Introductionmentioning

confidence: 91%

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Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

Lorenzoni¹,

Fracalossi²,

Carlin³

et al. 2012

The Angle Orthodontist

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Objectives: To evaluate the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated buccal mucosa cells from children undergoing orthodontic radiographs. Materials and Methods: A total of 25 healthy children undergoing orthodontic therapy partook in a complete set of orthodontic radiographs (lateral cephalographic, posteroanterior cephalographic, panoramic, full periapical exam, and bitewing). The micronucleus test in the buccal exfoliated cells was applied. The paired-samples t-test and the Wilcoxon test were used to compare the frequencies of alterations before and after X-ray exposure. Results: We found no statistically significant differences (P . .05) between micronucleated buccal mucosa cells before and after exposure to radiation. However, radiation did cause other nuclear alterations closely related to cytotoxicity (P 5 .007). Conclusion: According to the micronucleus test, the complete set of radiographs requested in the orthodontic planning may not be a factor that induces chromosomal damage, but it is able to promote cytotoxicity. (Angle Orthod. 2012;82:585-590.)

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

See 3 more Smart Citations

Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

Lorenzoni¹,

Fracalossi²,

Carlin³

et al. 2012

The Angle Orthodontist

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Comparative evaluation of mutagenic effects of two cone‐beam computed tomography in oral mucosa cells

Ayres

Santos

Santana

et al. 2023

Diagnostic Cytopathology

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BackgroundThis pilot study aimed to evaluate the mutagenic effects in cells of the oral mucosa after exposure to two different cone beam computed tomography (CBCT).MethodsEighteen adults were submitted to the different CBCT (Carestream CS8100 3D and I‐CAT). The cells were collected immediately before the CBCT exposure and 10 days later, when the material was placed on a slide and stained using the Feulgen/Fast Green technique. Microscopic analysis counted micronuclei and other nuclear alterations, which are indicative of cytotoxicity such as pyknosis, karyolysis, karyorrhexis, and binucletion. 2000 cells were analyzed. The statistical analysis was performed with the Wilcoxon Signed‐Rank test to compare the frequency of cellular alterations, and the Mann–Whitney U test to compare different CBCTs, both with a significance level of 5%.ResultsThere was no statistically significant difference in the micronucleated cell count before and after the exposition to the ionizing radiation from I‐CAT (p = .298) and CS8100 3D (p = .203) A significate increase of pyknosis (p < .001), karyolysis (p < .001), karyorrhexis (p < .001), and binucletion (p < .001) were noted on I‐CAT CBCT. There was no statistically significant difference in cellular alterations in CS8100 3D CBCT.ConclusionDespite the increase in micronuclei after exposure, this study indicates that there is no evidence of genotoxicity. On the other hand, the I‐CAT CBCT produced cytotoxic effects.

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Is micronucleus assay a useful marker in gingiva, tongue, and palate for evaluating cytogenetic damage induced by chemical, physical, and biological agents in vivo? A systematic review with meta‐analysis

Pinto,

Takeshita,

Renno

et al. 2024

J of Applied Toxicology

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The present systematic review (SR) aims to evaluate manuscripts in order to help further elucidate the following question: is the micronucleus assay (MA) also a useful marker in gingiva, tongue, and palate for evaluating cytogenetic damage in vivo? A search was performed through the electronic databases PubMed/Medline, Scopus, and Web of Science, all studies published up to December 2023. The comparisons were defined as standardized mean difference (SMD), and 95% confidence intervals (CIs) were established. Full manuscripts from 34 studies were carefully selected and reviewed in this setting. Our results demonstrate that the MA may be a useful biomarker of gingival tissue damage in vivo, and this tissue could be a useful alternative to the buccal mucosa. The meta‐analysis analyzing the different sites regardless of the deleterious factor studied, the buccal mucosa (SMD = 0.69, 95% CI, − 0.49 to 1.88, p = 0.25) and gingiva (SMD = 0.31, 95% CI, − 0.11 to 0.72, p = 0.15), showed similar results and different outcome for the tongue (SMD = 1.19, 95% CI, 0.47 to 1.91, p = 0.001). In summary, our conclusion suggests that the MA can be a useful marker for detecting DNA damage in gingiva in vivo and that this tissue could be effective site for smearing.

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Nuclear changes in tongue epithelial cells following panoramic radiography

Cited by 34 publications

References 15 publications

Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

Comparative evaluation of mutagenic effects of two cone‐beam computed tomography in oral mucosa cells

Is micronucleus assay a useful marker in gingiva, tongue, and palate for evaluating cytogenetic damage induced by chemical, physical, and biological agents in vivo? A systematic review with meta‐analysis

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