Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

Lorenzoni, Diego Coelho; Fracalossi, Ana Carolina Cuzzuol; Carlin, Viviane; Ribeiro, Daniel Araki; Sant’Anna, Eduardo Franzotti

doi:10.2319/072311-468.1

Cited by 18 publications

(18 citation statements)

References 32 publications

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“…This included lateral and posteroanterior cephalographics, panoramic, full periapical exams and bitewing exams. The MN test indicated no increase in chromosomal damage, however, a significant increase in nuclear alterations related to cytotoxicity was observed similar to results observed in adult patients (Lorenzoni et al 2012). The study was repeated with CBCT and similar findings were observed (Lorenzoni et al 2013).…”

Section: Cell-based Studiessupporting

confidence: 81%

A comprehensive review of the literature on the biological effects from dental X-ray exposures

Chauhan

Wilkins

2019

International Journal of Radiation Biology

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Purpose: Routine dental X-rays are among the most common sources of ionizing radiation exposure for healthy individuals globally, with 300 examinations/1000 individuals/year as documented by the United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR) global survey of medical radiation usage and exposure. Furthermore, in the United States of America, an increased use of dental radiography is evident. However, with the shift from using film to digital image receptors, the dose of radiation per routine examination has decreased. Despite this, there remains public concern of dental X-rays. This continuing concern highlights the need to review the literature on biological and health effects related to exposure, from dental X-rays. Material and methods: This report presents studies investigating biological and health effects related to exposures using dental X-rays in patients and provides a critical evaluation. Relevant studies specific to dental X-rays are reviewed from 1970 and onward with the bulk data in this field resulting from epidemiological and biomonitoring studies. Results: While, most epidemiological studies suggest a possible correlation between head/neck related tumors and exposure to dental X-rays, evidence for causation is lacking. Biomonitoring studies suggest that exposure to low-level radiation such as that of dental radiography may not be a factor in inducing long-term chromosomal damage, but may result in localized cytotoxicity in the irradiated region of the mouth, with no long-term harm. Conclusions: In general, the total number of studies is low and the majority of the data has been generated from poorly designed experiments. This review will highlight shortcomings that could influence outcomes and provide a more balanced interpretation that could impact the public perception and the level of public concern on the health effects resulting from dental X-rays.

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Section: Cell-based Studiessupporting

confidence: 81%

A comprehensive review of the literature on the biological effects from dental X-ray exposures

Chauhan

Wilkins

2019

International Journal of Radiation Biology

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“…When frequencies of nuclear anomalies of condensed chromatin and karyorrhectic and pyknotic cells were compared in different healthy children populations, large variabilities were observed. While some of them (40,50), including our study, had much higher values, others (51) had lower values than the levels obtained in the study by Thomas et al (52). The causes of this variability may, in part, reflect genetic and non-specific exposure differences, or in part, technical factors and differences in the sample preparation and interpretation of the scoring criteria between laboratories.…”

Section: Resultscontrasting

confidence: 54%

“…The previously published studies that have investigated directly the effects of several radiographic and cephalographic techniques in dental practices (e.g. panoramic dental radiography, lateral cephalography, post anterior cephalography, and full-mouth X-ray) on the target tissue BEC collected ten days after X-ray exposure, which is the likely maximal rate of MN occurrence, have linked X-ray exposure to cytotoxicity but not genotoxicity determined as MN frequencies (39)(40)(41)(42). In all these studies, an X-ray dose was between 0.003 mSv and 0.02 mSv (1-4).…”

Section: Resultsmentioning

confidence: 99%

Micronuclei and other nuclear anomalies in buccal epithelial cells of children with chronic kidney disease

Aykanat

Demircigil

Buyan

et al. 2016

Archives of Industrial Hygiene and Toxicology

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The objective of this study was to reveal the likely genomic instability in children with chronic kidney disease (CKD) using micronucleus (MN) assay on buccal epithelial cells (BEC). We investigated the frequencies of micronuclei and other nuclear anomalies, such as nuclear buds, binucleated cells, condensed chromatin, and karyorrhectic and pyknotic cells in BEC. Children with CKD were grouped as follows: children in the pre-dialysis (PreD) stage (N=17), children on regular haemodialysis (HD) (N=14), and children who have undergone transplantation (Tx) (N=17). As a control group, twenty age-and gender-matched healthy children were selected. The MN frequency in BEC of all groups of children with CKD was significantly elevated (5-to 7-fold) as compared to the control group (p<0.001). In contrast, the frequencies of nuclear buds were not significantly higher in the study groups compared to the control group. The frequencies of binucleated cells and condensed chromatin cells were significantly higher in all subgroups of children with CKD relative to the control group (p<0.001). Our results show that the BEC of pediatric PreD, HD, and Tx patients with CKD display increased cytogenetic, cytokinetic, and cytotoxic effects. They also point to the sensitivity and usefulness of the BEC MN assay in the assessment of genetic susceptibility of patients with CKD.

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“…In all these studies comparisons were made of MN within subjects before and shortly after (generally within a few days) of X-ray exposure. It is interesting that although negative for MN, the studies of Ribeiro et al [45, 47], Angelieri et al [46, 49] and Lorenzoni et al [48] showed increases in the cytotoxic endpoints of karrhyorexis, pyknosis, and karyolysis after X-ray exposure, suggesting a substantial degree of induced cell death among buccal mucosa. This may explain the reduced MN frequency that we observed, presumably a much longer time after bone X-ray exposure than in these earlier studies.…”

Section: Discussionmentioning

confidence: 99%

Buccal mucosa micronuclei counts in relation to exposure to low dose-rate radiation from the Chornobyl nuclear accident and other medical and occupational radiation exposures

Bazyka¹,

Finch²,

Ilienko³

et al. 2017

Environ Health

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BackgroundIonizing radiation is a well-known carcinogen. Chromosome aberrations, and in particular micronuclei represent an early biological predictor of cancer risk. There are well-documented associations of micronuclei with ionizing radiation dose in some radiation-exposed groups, although not all. That associations are not seen in all radiation-exposed groups may be because cells with micronuclei will not generally pass through mitosis, so that radiation-induced micronuclei decay, generally within a few years after exposure.MethodsBuccal samples from a group of 111 male workers in Ukraine exposed to ionizing radiation during the cleanup activities at the Chornobyl nuclear power plant were studied. Samples were taken between 12 and 18 years after their last radiation exposure from the Chornobyl cleanup. The frequency of binucleated micronuclei was analyzed in relation to estimated bone marrow dose from the cleanup activities along with a number of environmental/occupational risk factors using Poisson regression adjusted for overdispersion.ResultsAmong the 105 persons without a previous cancer diagnosis, the mean Chornobyl-related dose was 59.5 mSv (range 0–748.4 mSv). There was a borderline significant increase in micronuclei frequency among those reporting work as an industrial radiographer compared with all others, with a relative risk of 6.19 (95% CI 0.90, 31.08, 2-sided p = 0.0729), although this was based on a single person. There was a borderline significant positive radiation dose response for micronuclei frequency with increase in micronuclei per 1000 scored cells per Gy of 3.03 (95% CI -0.78, 7.65, 2-sided p = 0.1170), and a borderline significant reduction of excess relative MN prevalence with increasing time since last exposure (p = 0.0949). There was a significant (p = 0.0388) reduction in MN prevalence associated with bone X-ray exposure, but no significant trend (p = 0.3845) of MN prevalence with numbers of bone X-ray procedures.ConclusionsThere are indications of increasing trends of micronuclei prevalence with Chornobyl-cleanup-associated dose, and indications of reduction in radiation-associated excess prevalence of micronuclei with time after exposure. There are also indications of substantially increased micronuclei associated with work as an industrial radiographer. This analysis adds to the understanding of the long-term effects of low-dose radiation exposures on relevant cellular structures and methods appropriate for long-term radiation biodosimetry.Electronic supplementary materialThe online version of this article (doi:10.1186/s12940-017-0273-x) contains supplementary material, which is available to authorized users.

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Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

Cited by 18 publications

References 32 publications

A comprehensive review of the literature on the biological effects from dental X-ray exposures

A comprehensive review of the literature on the biological effects from dental X-ray exposures

Micronuclei and other nuclear anomalies in buccal epithelial cells of children with chronic kidney disease

Buccal mucosa micronuclei counts in relation to exposure to low dose-rate radiation from the Chornobyl nuclear accident and other medical and occupational radiation exposures

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