Novel sources of witches’ broom resistance (causal agent Moniliophthora perniciosa) from natural populations of Theobroma cacao from the Brazilian Amazon

Albuquerque, Paulo; Silva, Stela D. V. M.; Luz, Edna Dora Martins Newman; Pires, J.L.; Vieira, Afrânio Márcio Corrêa; Demétrio, Clarice Garcia Borges; Pascholatti, Sérgio F.; Figueira, Antônio

doi:10.1007/s10681-009-0068-4

Cited by 23 publications

(15 citation statements)

References 23 publications

(39 reference statements)

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“…This fact evidenced the evolutionary process of the pathogen with the increase in the pathogen population of types, which were more aggressive to SCA clones. Similar results were obtained by Albuquerque et al (2010). Pires et al (1999), in a study of association between resistance sources to WB for the increment of the level and of the durability of the trait, noted that SCA, when crossed with other resistant parents, maintained low level of infection in areas where the pathogen evolution was present, while progenies which had only SCA as resistant parent presented high level of infection, proving the increased resistance durability due to the combination of different genes.…”

Section: Cs Benjamin Et Alsupporting

confidence: 90%

Cacao families and parents selected as resistant to natural infection of Moniliophthora perniciosa

Benjamin

Luz

Santos

et al. 2016

Crop Breed. Appl. Biotechnol.

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Section: Cs Benjamin Et Alsupporting

confidence: 90%

Cacao families and parents selected as resistant to natural infection of Moniliophthora perniciosa

Benjamin

Luz

Santos

et al. 2016

Crop Breed. Appl. Biotechnol.

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“…Using the TcBI‐1 ‐specific primers based on the identified Unigene accession, the fragment (∼750 bp) was amplified from cDNA obtained from a resistant cacao genotype (‘CAB 214’; Albuquerque et al ., ) treated with Ethrel (Litholdo et al ., ). The fragment was cloned and recombined to obtain the binary vector pK7WG2D ::TcBI‐1 , and introduced into ‘Micro‐Tom’ (MT) tomato.…”

Section: Resultsmentioning

confidence: 99%

Expression of the Theobroma cacao Bax‐inhibitor‐1 gene in tomato reduces infection by the hemibiotrophic pathogen Moniliophthora perniciosa

Scotton

Azevedo

Sestari

et al. 2016

Molecular Plant Pathology

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Programmed cell death (PCD) plays a key role in plant responses to pathogens, determining the success of infection depending on the pathogen lifestyle and on which participant of the interaction triggers cell death. The hemibiotrophic basidiomycete Moniliophthora perniciosa is the causal agent of witches' broom disease of Theobroma cacao L. (cacao), a serious constraint for production in South America and the Caribbean. It has been hypothesized that M. perniciosa pathogenesis involves PCD, initially as a plant defence mechanism, which is diverted by the fungus to induce necrosis during the dikaryotic phase of the mycelia. Here, we evaluated whether the expression of a cacao anti-apoptotic gene would affect the incidence and severity of M. perniciosa infection using the 'Micro-Tom' (MT) tomato as a model. The cacao Bax-inhibitor-1 (TcBI-1) gene, encoding a putative basal attenuator of PCD, was constitutively expressed in MT to evaluate function. Transformants expressing TcBI-1, when treated with tunicamycin, an inducer of endoplasmic reticulum stress, showed a decrease in cell peroxidation. When the same transformants were inoculated with the necrotrophic fungal pathogens Sclerotinia sclerotiorum, Sclerotium rolfsii and Botrytis cinerea, a significant reduction in infection severity was observed, confirming TcBI-1 function. After inoculation with M. perniciosa, TcBI-1 transformant lines showed a significant reduction in disease incidence compared with MT. The overexpression of TcBI-1 appears to affect the ability of germinating spores to penetrate susceptible tissues, restoring part of the non-host resistance in MT against the S-biotype of M. perniciosa.

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“…The introduction of witches' broom disease into cocoa producing areas of South America and Caribbean has devastated the chocolate industry [6]. The ultimate method of witches' broom control [7] will be through genetic resistance, therefore the resistance source "Scavina 6" is not doing very well in field conditions, leading to fungi resistant strains [8] [9]. Moniliophthora perniciosa is a hemibiotrophic pathogen, with contrasting mycelial morphology and behavior during the biotrophic and necrotrophic phases [5] Its biotrophic phase starts with the germination of a basidiospore, apoplast colonization with no specific infection structure [10].…”

Section: Introductionmentioning

confidence: 99%

Alpha-Tomatin against Witches’ Broom Disease

Gomes¹,

Duarte²,

Andrino³

et al. 2014

AJPS

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Several plants use secondary metabolites against insects and pathogens attack. Among such metabolites known glycoalkaloids are the most studied. Plants from Solanaceae Family are the most abundant on those alkaloids. Despite alfa-tomatine is a known glycoalkaloid, its specific action against Moniliophthora perniciosa, fungi responsible for witches broom disease in cocoa plantations was obtained in this work. Alfa-tomatine was infusion-extracted from Solanum lycopersicum leaves, and its action against the fungi growth was shown on the bioassays. In these tests, M. perniciosa growth was totally inhibited but other fungus tested, the inhibition was not totally, or did not present effect on growth, showing in this way, the specificity of alfa-tomatine to M. perniciosa. The advantages of work with a natural molecule for the fungi control is the stability of the molecule, its resistance to high temperature and pressure and for its use, the simple method of extraction used. The confirmation of the alfa-tomatine molecule was done by High Performance Liquid Chromatography and Mass Spectrometry in comparison to the Sigma standard.

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Novel sources of witches’ broom resistance (causal agent Moniliophthora perniciosa) from natural populations of Theobroma cacao from the Brazilian Amazon

Abstract: Witches' broom is a severe disease of Theobroma cacao L. (cacao), caused by the basidiomycete Moniliophthora perniciosa.

Cited by 23 publications

References 23 publications

Cacao families and parents selected as resistant to natural infection of Moniliophthora perniciosa

Cacao families and parents selected as resistant to natural infection of Moniliophthora perniciosa

Expression of the Theobroma cacao Bax‐inhibitor‐1 gene in tomato reduces infection by the hemibiotrophic pathogen Moniliophthora perniciosa

Alpha-Tomatin against Witches’ Broom Disease

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