Nonomuraea rhodomycinica sp. nov., isolated from peat swamp forest soil

Abstract

The taxonomic position of an actinomycete, strain NR4-ASC07T, isolated from a soil sample collected from Sirindhorn peat swamp forest, Narathiwat Province, Thailand, was clarified using a polyphasic approach. On the basis of morphological and chemotaxonomic characteristics, it was classified among the members of the genus Nonomuraea. It produced tightly closed spiral spore chains on aerial mycelium as well as forming a pseudosporangium. Whole-cell hydrolysates contained meso-diaminopimelic acid, glucose, ribose, madurose and mannose. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, unknown ninhydrin-positive phosphoglycolipids and unknown glycolipid. Menaquiones were MK-9(H4), MK-9(H0), MK-9(H2), MK-10(H4) and MK-9(H6). Predominant cellular fatty acids were iso-C16 : 0, C17 : 0 10-methyl, C16 : 0, C17 : 1ω8c, C16 : 0 2-OH and iso-C15 : 0. The phylogenetic tree reconstructed on the basis of 16S rRNA gene sequences showed that the strain fell within the clade containing Nonomuraea muscovyensis FMN03T, Nonomuraea roseoviolacea subsp. roseoviolaceaNBRC 14098T and Nonomuraea roseoviolacea subsp. carminataNBRC 15903T. The DNA-DNA relatedness and phenotypic data supported that strain NR4-ASC07T was clearly distinguished from the closely related species and represents a novel species of the genus Nonomuraea for which the name Nonomuraea rhodomycinica sp. nov. is proposed. The type strain is NR4-ASC07T (=NBRC 112327T=TISTR 2465T).

Nonomuraea montanisoli sp. nov., isolated from mountain forest soil

“…The G+C content of the genomic DNA is in the range of 64 to 73 mol%. The species of this genus have been isolated from a wide variety of natural habitats such as soils [4], coastal sediment [5], cave soils [6], rhizospheres [7] and plant tissues [8]. The genus consisted of 70 species and two subspecies, based on the LPSN (List of Prokaryotic names with Standing in Nomenclature, http://www.bacterio.cict.fr/index.html) website; last updated on 9 November 2020.…”

Nonomuraea insulae sp. nov., isolated from forest soil

“…and have diphosphatidylglycerol, phosphatidylethanolamine and hydroxylated phosphatidylethanolamine as predominant phospholipids (Kämpfer 2012). Members of the genus Nonomuraea have been recently isolated from various habitats such as soil (Wang et al 2014;Sripreechasak et al 2017), rhizosphere (Shen et al 2016;Wang et al 2017), cave (Fang et al 2017), root (Rachniyom et al 2015;Li et al 2017), coastal sediment (Xi et al 2011), plant tissues (Li et al 2011;Niemhom et al 2017) and mangrove sediment (Suksard et al 2016). Additionally, it is known that some secondary metabolites such as maduramycin, pyralomicin, the glycopeptide antibiotic A40926 and brartemicin are produced by members of the genus Nonomuraea (Sungthong and Nakaew 2015;Dalmastri et al 2016).…”

Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov.

“…In addition, it is known that Nonomuraea rosea, Nonomuraea roseoviolacea, Nonomuraea rubra, Nonomuraea pusilla, Nonomuraea spiralis and Nonomuraea gerenzanensis produce deoxycephalomycin B, carminomicins, maduromycin, actinotiocin, pyralomicin and glycopeptide antibiotic A40926 [9][10][11][12][13][14][15]. Members of the genus have been isolated from diverse natural habitats such as rhizosphere [16,17], leaf [18], cave [19], limestone mine [20], root [5,21], coastal sediment [22], mangrove sediment [23] and mushroom compost [24], while many members were isolated from different types of soil including cave soil [25], forest soil [26,27], arid soil [28], acidic soil [29], rhizosphere soil [3,[30][31][32] and mangrove soil [33].…”