Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov.

Saygin, Hayrettin; Nouioui, Imen; Ay, Hilal; Güven, Kıymet; Çetin, Demet; Klenk, Hans‐Peter; Goodfellow, Michael; Şahi̇n, Nevzat

doi:10.1099/ijsem.0.003808

Cited by 418 publications

(4 citation statements)

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“…The 16S rRNA gene of strain 80 T was amplified by PCR using forward primer 27F (5′-AGAGTTTGATC(AC)TGGCTCAG-3′) and reverse primer 1492R(5′-ACGG(CT)TACCTTGTTACGACTT-3′) [14]. After sequencing, the nucleotide sequence similarities were compared using blast on the NCBI (National Center for Biotechnology Information, www.ncbi.nlm.nih.gov/) and EzBioCloud Databases (http://eztaxon-e.ezbiocloud.net) [15].…”

Section: Phylogeny and Genome Featuresmentioning

confidence: 99%

Radiobacillus kanasensis sp. nov., a halotolerant bacterium isolated from woodland soil

Zhang

Huang

Wang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-positive, aerobic, rod-shaped, non-motile, endospore-forming salt-tolerant bacterium strain (80T), was isolated from woodland soil collected near Kanas lake in the Altay region of Xinjiang, PR China. The strain grew at 15−45 °C, pH6.0–9.0 and with 0–14 % (w/v) NaCl. The complete genome size of the novel strain was 4 031 766 bp including a circle chromosome and a circle plasmid. The genomic DNA G+C content was 38.99 mol %. Phylogenetic analysis based on 16S rRNA gene sequence and genome showed that strain 80T has the highest similarity to Radiobacillus deserti TKL69T. However, the novel strain showed an average nucleotide identity value of 78.65 % (lower than 95 %) and a digital DNA–DNA hybridization value of 22.30 % with R. deserti TKL69T based on the genome sequences. The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, anteiso-C17:0 and C16 : 1 ω7c alcohol. The only respiratory quinone was MK-7. The cell wall peptidoglycan was meso-diaminopimelic acid. Diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid, one unidentified aminophospholipid and two unidentified glycolipids were identified as the major polar lipids. The phylogenetic, phenotypic and chemotaxonomic analyses showed that strain 80T represents a novel species of the genus Radiobacillus and the name Radiobacillus kanasensis sp. nov. is proposed. The type strain is 80T (=GDMCC 1.2844T=JCM 35077T).

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Section: Phylogeny and Genome Featuresmentioning

confidence: 99%

Radiobacillus kanasensis sp. nov., a halotolerant bacterium isolated from woodland soil

Zhang

Huang

Wang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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“…Differential characteristics of strain NN258 T and type strains of closely related species Strain: 1, NN258 T ; 2, Nonomuraea candida JCM 15928 T ; 3, Nonomuraea mesophila 6K102 T ; 4, Nonomuraea rubra JCM T ; 5, Nonomuraea diastatica KC712 T . All data were obtained in the present study except data for N. mesophila and N. diastatica, (data taken from Saygin et al [6]). +, Positive; -, negative.…”

Section: Morphology and Physiologymentioning

confidence: 99%

“…G+C content ranges from 64 to 73 mol%. Members of the genus are widely distributed and have been isolated from various natural habitats such as plant roots, terrestrial soils, mangrove sediments and cave soils [3,[6][7][8][9]. At the time of writing, the genus comprises 59 species with validly published names (https:// lpsn.…”

Section: Introductionmentioning

confidence: 99%

“…The genus description has been subjected to repeated emendations by Nakaew et al [3] and Cao et al [4]. Members of the genus are aerobic, Gram-stain-positive, non-motile actinomycetes, which form hooked, spiral or straight spore chains with smooth or rough surfaces on an extensively branched substrate and aerial mycelium [5, 6]. The cell wall contains meso -diaminopimelic acid with madurose as the diagnostic sugar in the whole-cell hydrolysate.…”

Section: Introductionmentioning

confidence: 99%

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Nonomuraea antri sp. nov., an actinomycete isolated from cave soil in Thailand

Lipun

Teo

Suksaard

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A novel actinobacterium, designated strain NN258T, was isolated from a cave soil sample collected from a karst cave at Khao No-Khao Kaeo, Nakhon Sawan province, Thailand. The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Nonomuraea . Strain NN258T showed the highest 16S rRNA gene sequence similarity values to Nonomuraea candida HMC10T, Nonomuraea mesophila 6K102T, Nonomuraea rubra DSM 43768T, Nonomuraea diastatica KC712T and Nonomuraea helvata IFO 14681T. The strain formed an extensively branched substrate and aerial mycelia. The whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, with glucose, madurose, mannose and ribose as the whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphotidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, two unidentified phospholipids, three unidentified sugar-containing phosphoaminolipids, an unidentified glycolipid and two unidentified lipids. The predominant menaquinone was MK-9(H4), with minor amounts of MK-9(H0), MK-9(H2) and MK-9(H6). Major cellular fatty acids (>10%) were iso-C16 : 0 and 10-methyl-C17 : 0. The G+C content of the genomic DNA was 71.0 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain NN258T and the reference strains were 79.9–80.9 % and 26.1–27.0 %, respectively. On the basis of phenotypic, genotypic and phylogenetic data, strain NN258T represents a novel species of the genus Nonomuraea , for which the name Nonomuraea antri sp. nov. is proposed. The type strain is NN258T (=TBRC 11478T=NBRC 114269T).

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