Nonomuraea antri sp. nov., an actinomycete isolated from cave soil in Thailand

Abstract

A novel actinobacterium, designated strain NN258T, was isolated from a cave soil sample collected from a karst cave at Khao No-Khao Kaeo, Nakhon Sawan province, Thailand. The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Nonomuraea . Strain NN258T showed the highest 16S rRNA gene sequence similarity values to Nonomuraea candida HMC10T, Nonomuraea mesophila 6K102T, Nonomuraea rubra DSM 43768T, Nonomuraea diastatica KC712T and Nonomuraea helvata IFO 14681T. The strain formed an extensively branched substrate and aerial mycelia. The whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, with glucose, madurose, mannose and ribose as the whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphotidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, two unidentified phospholipids, three unidentified sugar-containing phosphoaminolipids, an unidentified glycolipid and two unidentified lipids. The predominant menaquinone was MK-9(H4), with minor amounts of MK-9(H0), MK-9(H2) and MK-9(H6). Major cellular fatty acids (>10%) were iso-C16 : 0 and 10-methyl-C17 : 0. The G+C content of the genomic DNA was 71.0 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain NN258T and the reference strains were 79.9–80.9 % and 26.1–27.0 %, respectively. On the basis of phenotypic, genotypic and phylogenetic data, strain NN258T represents a novel species of the genus Nonomuraea , for which the name Nonomuraea antri sp. nov. is proposed. The type strain is NN258T (=TBRC 11478T=NBRC 114269T).

Nonomuraea aurantiaca sp. nov., a novel cellulase-producing actinobacterium isolated from soil

“…The major phospholipids are diphosphatidylglycerol, phosphatidylethanolamine, hydroxylated phosphatidylethanolamine and ninhydrin- and sugar-positive phospholipids except for several described species that lack glucosamine-containing phospholipid (GluNu) [4, 11, 12]. At the time of writing, the genus Nonomuraea contains 65 species with validly published names (https://lpsn.dsmz.de/genus/nonomuraea), including the latest described species, Nonomuraea antri [13] and Nonomuraea terrae [14]. In the process of investigating novel actinobacteria from soil, strain NEAU-L178 T was isolated.…”

Nonomuraea montanisoli sp. nov., isolated from mountain forest soil

“…The G+C content of the genomic DNA is in the range of 64 to 73 mol%. The species of this genus have been isolated from a wide variety of natural habitats such as soils [4], coastal sediment [5], cave soils [6], rhizospheres [7] and plant tissues [8]. The genus consisted of 70 species and two subspecies, based on the LPSN (List of Prokaryotic names with Standing in Nomenclature, http://www.bacterio.cict.fr/index.html) website; last updated on 9 November 2020.…”